Fz7-21 TFA
Based on 3 publication(s) in Google Scholar
Fz7-21 (Ac-LPSDDLEFWCHVMY-NH2) TFA is a potent peptide antagonist of FZD7 receptors , selectively binds to FZD7 CRD subclass and alters the conformation of the CRD and the architecture of its lipid-binding groove. The EC50 values are 58 and 34 nM for human and mouse FZD7 CRD, respectively. Fz7-21 TFA impairs the function of FZD7 in Wnt-β-catenin signalling and stem cell function in intestinal organoids.
연구목적의 판매만을 진행합니다. 환자를 대상으로 한 판매는 하지 않습니다.
- Purity: 98.12%
- 화학식: C85H115N18F3O25S2
- 분자량:1910.07
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보관:
Sealed storage, away from moisture.
Powder -80°C, 2 years , -20°C, 1 year* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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용액&용해도
- 순도&문서
- References
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Help & FAQs
Publications Citing Use of MedChemExpress (MCE) Fz7-21 TFA
More
Customer Validation & Images
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IF
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IF
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WB
Biological Activity
EC50: 58 nM (human FZD7 CRD), 34 nM (mouse FZD7 CRD)[1]
Fz7-21 (Ac-LPSDDLEFWCHVMY-NH2) TFA (0-100 μM; 6 h; HEK293-TB cells) impairs Wnt signaling with IC50 value of 100 nM[1].
Fz7-21 (Ac-LPSDDLEFWCHVMY-NH2) TFA (1 μM) blocks WNT3A-mediated stabilization of β-catenin in mouse L cells with IC50 value of 50 nM[1].
Fz7-21 (Ac-LPSDDLEFWCHVMY-NH2) TFA (200 μM; 48 h) disrupts LGR5+ stem cell function[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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Appearance Solid
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분자량 1910.07
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화학식 C85H115N18F3O25S2
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Color White to off-white
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Synonyms
Ac-LPSDDLEFWCHVMY-NH2 TFA
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Sequence Shortening
Ac-LPSDDLEFWCHVMY-NH2
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선적
Room temperature in continental US; may vary elsewhere.
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보관
Sealed storage, away from moisture
Powder -80°C 2 years -20°C 1 year * In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
Publications (3)
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Journal Impact Factor
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Most Recent
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Stem Cell Res Ther
Regulation of self-renewal and senescence in primitive mesenchymal stem cells by Wnt and TGFβ signaling. [Abstract]2023 Oct 26;14(1):305. PMID: 37880755 -
Cell Mol Life Sci
L-glutamate requires β-catenin signalling through Frizzled7 to stimulate porcine intestinal stem cell expansion. [Abstract]2022 Sep 19;79(10):523. PMID: 36121491 -
Cell Prolif
WNT16b promotes the proliferation and self-renewal of human limbal epithelial stem/progenitor cells via activating the calcium/calcineurin A/NFATC2 pathway. [Abstract]2023 Oct;56(10):e13460. PMID: 36974338
Fz7-21 TFA purchased from MedChemExpress. Usage Cited in: Cell Prolif. 2023 Oct;56(10):e13460. [Abstract]
Fz7-21 (200 nM; 14 days) downregulates the expression of the stemness biomarker ΔNp63α in hLESCs.
Fz7-21 TFA purchased from MedChemExpress. Usage Cited in: Cell Prolif. 2023 Oct;56(10):e13460. [Abstract]
Fz7-21 (200 nM; 14 days) inhibits the release of Ca2+ induced by WNT16b in hLESCs.
Fz7-21 TFA purchased from MedChemExpress. Usage Cited in: Cell Prolif. 2023 Oct;56(10):e13460. [Abstract]
Fz7-21 (200 nM; 14 days) suppresses hLESC proliferation induced by WNT16b in hLESCs.
Fz7-21 TFA purchased from MedChemExpress. Usage Cited in: Cell Prolif. 2023 Oct;56(10):e13460. [Abstract]
Fz7-21 (200 nM; 14 days) decreases the expression of Calcineurin A and NFATC2 in hLESCs.
용액&용해도
DMSO : 33.33 mg/mL (17.45 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
H2O : < 0.1 mg/mL (insoluble)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: 2.5 mg/mL (1.31 mM); Suspended solution; Need ultrasonic
This protocol yields a suspended solution of 2.5 mg/mL. Suspended solution can be used for oral and intraperitoneal injection.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL. * In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
순도&문서
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Data Sheet (271 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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Handling Instructions (2659 KB)
References
[1]. Nile AH, et, al. A selective peptide inhibitor of Frizzled 7 receptors disrupts intestinal stem cells. Nat Chem Biol. 2018 Jun;14(6):582-590. [Content Brief]
[2]. Larasati Y, et, al. Unlocking the Wnt pathway: Therapeutic potential of selective targeting FZD7 in cancer. Drug Discov Today. 2022 Mar;27(3):777-792. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 0.5235 mL | 2.6177 mL | 5.2354 mL | 13.0885 mL |
| 5 mM | 0.1047 mL | 0.5235 mL | 1.0471 mL | 2.6177 mL | |
| 10 mM | 0.0524 mL | 0.2618 mL | 0.5235 mL | 1.3089 mL | |
| 15 mM | 0.0349 mL | 0.1745 mL | 0.3490 mL | 0.8726 mL |
Fz7-21 TFA Related Classifications
HY-P1454A Related Classifications
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