Fz7-21
Based on 3 publication(s) in Google Scholar
Fz7-21 (Ac-LPSDDLEFWCHVMY-NH2) is a potent peptide antagonist of FZD7 receptors , selectively binds to FZD7 CRD subclass and alters the conformation of the CRD and the architecture of its lipid-binding groove. The EC50 values are 58 and 34 nM for human and mouse FZD7 CRD, respectively. Fz7-21 impairs the function of FZD7 in Wnt–β-catenin signalling and stem cell function in intestinal organoids.
For research use only. We do not sell to patients.
- Purity: 99.31%
- CAS No.: 2247635-23-8
- Formula: C83H114N18O23S2
- Molecular Weight:1796.05
-
Storage:
Sealed storage, away from moisture and light.
Powder -80°C, 2 years , -20°C, 1 year* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)
-
Biological Activity
-
Chemical Information
-
Solvent & Solubility
- Purity & Documentation
- References
-
Help & FAQs
Publications Citing Use of MedChemExpress (MCE) Fz7-21
More
Customer Validation & Images
-
IF
-
IF
-
IF
-
WB
Biological Activity
EC50: 58 nM (human FZD7 CRD), 34 nM (mouse FZD7 CRD)[1]
Fz7-21 (Ac-LPSDDLEFWCHVMY-NH2) (0-100μM; 6 h; HEK293-TB cells) impairs Wnt signaling with IC50 value of 100?nM[1].
Fz7-21 (Ac-LPSDDLEFWCHVMY-NH2) (1?μM) blocks WNT3A-mediated stabilization of β-catenin in mouse L cells with IC50 value of 50?nM[1].
Fz7-21 (Ac-LPSDDLEFWCHVMY-NH2) (200 μM; 48 h; LGR5–GFP+ stem cells) disrupts LGR5+ stem cell function[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
-
CAS No. 2247635-23-8
-
Appearance Solid
-
Molecular Weight 1796.05
-
Formula C83H114N18O23S2
-
Color White to off-white
-
Synonyms
Ac-LPSDDLEFWCHVMY-NH2
-
Sequence Shortening
Ac-LPSDDLEFWCHVMY-NH2
-
Shipping
Room temperature in continental US; may vary elsewhere.
-
Storage
Sealed storage, away from moisture and light
Powder -80°C 2 years -20°C 1 year * In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)
Publications (3)
-
Journal Impact Factor
-
Most Recent
-
Stem Cell Res Ther
Regulation of self-renewal and senescence in primitive mesenchymal stem cells by Wnt and TGFβ signaling. [Abstract]2023 Oct 26;14(1):305. PMID: 37880755 -
Cell Mol Life Sci
L-glutamate requires β-catenin signalling through Frizzled7 to stimulate porcine intestinal stem cell expansion. [Abstract]2022 Sep 19;79(10):523. PMID: 36121491 -
Cell Prolif
WNT16b promotes the proliferation and self-renewal of human limbal epithelial stem/progenitor cells via activating the calcium/calcineurin A/NFATC2 pathway. [Abstract]2023 Oct;56(10):e13460. PMID: 36974338
Fz7-21 purchased from MedChemExpress. Usage Cited in: Cell Prolif. 2023 Oct;56(10):e13460. [Abstract]
Fz7-21 (200 nM; 14 days) downregulates the expression of the stemness biomarker ΔNp63α in hLESCs.
Fz7-21 purchased from MedChemExpress. Usage Cited in: Cell Prolif. 2023 Oct;56(10):e13460. [Abstract]
Fz7-21 (200 nM; 14 days) inhibits the release of Ca2+ induced by WNT16b in hLESCs.
Fz7-21 purchased from MedChemExpress. Usage Cited in: Cell Prolif. 2023 Oct;56(10):e13460. [Abstract]
Fz7-21 (200 nM; 14 days) suppresses hLESC proliferation induced by WNT16b in hLESCs.
Fz7-21 purchased from MedChemExpress. Usage Cited in: Cell Prolif. 2023 Oct;56(10):e13460. [Abstract]
Fz7-21 (200 nM; 14 days) decreases the expression of Calcineurin A and NFATC2 in hLESCs.
Solvent & Solubility
DMSO : 100 mg/mL (55.68 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Purity & Documentation
-
Data Sheet (271 KB)
-
SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Portuguese - PT (252 KB)
-
Handling Instructions (2659 KB)
References
[1]. Nile AH, et, al. A selective peptide inhibitor of Frizzled 7 receptors disrupts intestinal stem cells. Nat Chem Biol. 2018 Jun;14(6):582-590. [Content Brief]
[2]. Larasati Y, et, al. Unlocking the Wnt pathway: Therapeutic potential of selective targeting FZD7 in cancer. Drug Discov Today. 2022 Mar;27(3):777-792. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 0.5568 mL | 2.7839 mL | 5.5678 mL | 13.9194 mL |
| 5 mM | 0.1114 mL | 0.5568 mL | 1.1136 mL | 2.7839 mL | |
| 10 mM | 0.0557 mL | 0.2784 mL | 0.5568 mL | 1.3919 mL | |
| 15 mM | 0.0371 mL | 0.1856 mL | 0.3712 mL | 0.9280 mL | |
| 20 mM | 0.0278 mL | 0.1392 mL | 0.2784 mL | 0.6960 mL | |
| 25 mM | 0.0223 mL | 0.1114 mL | 0.2227 mL | 0.5568 mL | |
| 30 mM | 0.0186 mL | 0.0928 mL | 0.1856 mL | 0.4640 mL | |
| 40 mM | 0.0139 mL | 0.0696 mL | 0.1392 mL | 0.3480 mL | |
| 50 mM | 0.0111 mL | 0.0557 mL | 0.1114 mL | 0.2784 mL |
Powered by Bioz