2. Stem Cell/Wnt
  3. Wnt
  4. Fz7-21

Fz7-21  (Synonyms: Ac-LPSDDLEFWCHVMY-NH2)

Cat. No.: HY-P1454 Purity: 98.44%
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Fz7-21 (Ac-LPSDDLEFWCHVMY-NH2) is a potent peptide antagonist of FZD7 receptors , selectively binds to FZD7 CRD subclass and alters the conformation of the CRD and the architecture of its lipid-binding groove. The EC50 values are 58 and 34 nM for human and mouse FZD7 CRD, respectively. Fz7-21 impairs the function of FZD7 in Wnt–β-catenin signalling and stem cell function in intestinal organoids.

For research use only. We do not sell to patients.

Custom Peptide Synthesis

Fz7-21 Chemical Structure

Fz7-21 Chemical Structure

CAS No. : 2247635-23-8

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5 mg USD 290 In-stock
10 mg USD 460 In-stock
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Based on 7 publication(s) in Google Scholar

Other Forms of Fz7-21:

Fz7-21 Related Antibodies

Top Publications Citing Use of Products

    Fz7-21 purchased from MedChemExpress. Usage Cited in: Cell Prolif. 2023 Mar 27;e13460.  [Abstract]

    Fz7-21 (200 nM; 14 days) inhibits the release of Ca2+ induced by WNT16b in hLESCs.

    Fz7-21 purchased from MedChemExpress. Usage Cited in: Cell Prolif. 2023 Mar 27;e13460.  [Abstract]

    Fz7-21 (200 nM; 14 days) suppresses hLESC proliferation induced by WNT16b in hLESCs.

    Fz7-21 purchased from MedChemExpress. Usage Cited in: Cell Prolif. 2023 Mar 27;e13460.  [Abstract]

    Fz7-21 (200 nM; 14 days) decreases the expression of Calcineurin A and NFATC2 in hLESCs.

    Fz7-21 purchased from MedChemExpress. Usage Cited in: Cell Prolif. 2023 Mar 27;e13460.  [Abstract]

    Fz7-21 (200 nM; 14 days) downregulates the expression of the stemness biomarker ΔNp63α in hLESCs.

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    Description

    Fz7-21 (Ac-LPSDDLEFWCHVMY-NH2) is a potent peptide antagonist of FZD7 receptors , selectively binds to FZD7 CRD subclass and alters the conformation of the CRD and the architecture of its lipid-binding groove. The EC50 values are 58 and 34 nM for human and mouse FZD7 CRD, respectively. Fz7-21 impairs the function of FZD7 in Wnt–β-catenin signalling and stem cell function in intestinal organoids[1][2].

    IC50 & Target

    EC50: 58 nM (human FZD7 CRD), 34 nM (mouse FZD7 CRD)[1]
    In Vitro

    Fz7-21 (Ac-LPSDDLEFWCHVMY-NH2) (0-100μM; 6 h; HEK293-TB cells) impairs Wnt signaling with IC50 value of 100 nM[1].
    Fz7-21 (Ac-LPSDDLEFWCHVMY-NH2) (1 µM) blocks WNT3A-mediated stabilization of β-catenin in mouse L cells with IC50 value of 50 nM[1].
    Fz7-21 (Ac-LPSDDLEFWCHVMY-NH2) (200 µM; 48 h; LGR5–GFP+ stem cells) disrupts LGR5+ stem cell function[1].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    Fz7-21 Related Antibodies
    Molecular Weight

    1796.05

    Formula

    C83H114N18O23S2
    CAS No.
    Appearance

    Solid

    Color

    White to off-white

    Sequence Shortening

    Ac-LPSDDLEFWCHVMY-NH2
    Shipping

    Room temperature in continental US; may vary elsewhere.
    Storage

    Sealed storage, away from moisture and light

    Powder -80°C 2 years
    -20°C 1 year

    *In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

    Solvent & Solubility
    In Vitro: 

    DMSO : 100 mg/mL (55.68 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 0.5568 mL 2.7839 mL 5.5678 mL
    5 mM 0.1114 mL 0.5568 mL 1.1136 mL
    View the Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

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    Purity & Documentation
    References

    Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
    DMSO 1 mM 0.5568 mL 2.7839 mL 5.5678 mL 13.9194 mL
    5 mM 0.1114 mL 0.5568 mL 1.1136 mL 2.7839 mL
    10 mM 0.0557 mL 0.2784 mL 0.5568 mL 1.3919 mL
    15 mM 0.0371 mL 0.1856 mL 0.3712 mL 0.9280 mL
    20 mM 0.0278 mL 0.1392 mL 0.2784 mL 0.6960 mL
    25 mM 0.0223 mL 0.1114 mL 0.2227 mL 0.5568 mL
    30 mM 0.0186 mL 0.0928 mL 0.1856 mL 0.4640 mL
    40 mM 0.0139 mL 0.0696 mL 0.1392 mL 0.3480 mL
    50 mM 0.0111 mL 0.0557 mL 0.1114 mL 0.2784 mL
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    Fz7-21 Related Classifications

    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    Fz7-212247635-23-8Ac-LPSDDLEFWCHVMY-NH2WntFZD7conformationlipid-binding grooveFZD7 CRDWnt–β-cateninintestinal organoidsInhibitorinhibitorinhibit

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