2. Stem Cell/Wnt
  3. Wnt
  4. Fz7-21 TFA

Fz7-21 TFA  (Synonyms: Ac-LPSDDLEFWCHVMY-NH2 TFA)

Cat. No.: HY-P1454A Purity: 99.85%
COA Handling Instructions

Fz7-21 (Ac-LPSDDLEFWCHVMY-NH2) TFA is a potent peptide antagonist of FZD7 receptors , selectively binds to FZD7 CRD subclass and alters the conformation of the CRD and the architecture of its lipid-binding groove. The EC50 values are 58 and 34 nM for human and mouse FZD7 CRD, respectively. Fz7-21 TFA impairs the function of FZD7 in Wnt-β-catenin signalling and stem cell function in intestinal organoids.

For research use only. We do not sell to patients.

Custom Peptide Synthesis

Fz7-21 TFA Chemical Structure

Fz7-21 TFA Chemical Structure

Size Price Stock Quantity
5 mg USD 290 In-stock
Estimated Time of Arrival: December 31
10 mg USD 460 In-stock
Estimated Time of Arrival: December 31
50 mg USD 1350 In-stock
Estimated Time of Arrival: December 31
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Customer Review

Based on 1 publication(s) in Google Scholar

Other Forms of Fz7-21 TFA:

Top Publications Citing Use of Products

1 Publications Citing Use of MCE Fz7-21 TFA

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  • References

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Description

Fz7-21 (Ac-LPSDDLEFWCHVMY-NH2) TFA is a potent peptide antagonist of FZD7 receptors , selectively binds to FZD7 CRD subclass and alters the conformation of the CRD and the architecture of its lipid-binding groove. The EC50 values are 58 and 34 nM for human and mouse FZD7 CRD, respectively. Fz7-21 TFA impairs the function of FZD7 in Wnt-β-catenin signalling and stem cell function in intestinal organoids[1][2].

IC50 & Target

EC50: 58 nM (human FZD7 CRD), 34 nM (mouse FZD7 CRD)[1]
In Vitro

Fz7-21 (Ac-LPSDDLEFWCHVMY-NH2) TFA (0-100 μM; 6 h; HEK293-TB cells) impairs Wnt signaling with IC50 value of 100 nM[1].
Fz7-21 (Ac-LPSDDLEFWCHVMY-NH2) TFA (1 µM) blocks WNT3A-mediated stabilization of β-catenin in mouse L cells with IC50 value of 50 nM[1].
Fz7-21 (Ac-LPSDDLEFWCHVMY-NH2) TFA (200 µM; 48 h; LGR5–GFP+ stem cells) disrupts LGR5+ stem cell function[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.
Molecular Weight

1910.07

Appearance

Solid

Formula

C85H115N18F3O25S2
Sequence Shortening

Ac-LPSDDLEFWCHVMY-NH2
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

Sealed storage, away from moisture

Powder -80°C 2 years
-20°C 1 year

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
Solvent & Solubility
In Vitro: 

DMSO : 33.33 mg/mL (17.45 mM; Need ultrasonic)

H2O : < 0.1 mg/mL (insoluble)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 0.5235 mL 2.6177 mL 5.2354 mL
5 mM 0.1047 mL 0.5235 mL 1.0471 mL
10 mM 0.0524 mL 0.2618 mL 0.5235 mL
*Please refer to the solubility information to select the appropriate solvent.
In Vivo:
  • 1.

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in saline)

    Solubility: 2.5 mg/mL (1.31 mM); Suspended solution; Need ultrasonic

  • 2.

    Add each solvent one by one:  10% DMSO    90% corn oil

    Solubility: ≥ 2.5 mg/mL (1.31 mM); Clear solution

*All of the co-solvents are available by MCE.
Purity & Documentation
References
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Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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Keywords:

Fz7-21Ac-LPSDDLEFWCHVMY-NH2WntFZD7conformationlipid-binding grooveFZD7 CRDWnt–β-cateninintestinal organoidsInhibitorinhibitorinhibit

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