MDH1-IN-2
Based on 1 publication(s) in Google Scholar
MDH1-IN-2 (Compound 7c) is a selective MDH1 inhibitor, with IC50 values of 2.27 and 27.47 μM for MDH1 and MDH2, respectively. MDH1-IN-2 can reduce the generation of ROS by inhibiting the conversion of 2-Ketoglutaric acid (HY-W013636) to α-Hydroxyglutaric acid (HY-113038B) mediated by MDH1, thereby suppressing 2-Ketoglutaric acid (HY-W013636)-induced ferroptosis.
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- Purity: 98.70%
- CAS No.: 2143463-35-6
- 화학식: C25H33NO5
- 분자량:427.53
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보관:Powder -20°C, 3 years ; In solvent -80°C, 6 months , -20°C, 1 month
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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용액&용해도
- 순도&문서
- References
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Help & FAQs
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Immunology/Inflammation Compound Library
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Ferroptosis Compound Library
HY-L051
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Highly Selective Inhibitors Library
HY-L158
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HY-L162
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Publications Citing Use of MedChemExpress (MCE) MDH1-IN-2
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Customer Validation & Images
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Flow Cytometry
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Bio/Physico-chemical Assay
Biological Activity
MDH1-IN-2 (10 μM; pre-treatment) reduces intracellular ROS production and α-Hydroxyglutaric acid (HY-113038B) production in the supernatant induced by Dimethyl 2-oxoglutarate (HY-44134) in OCI-LY1 and OCI-LY10 cells[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 2143463-35-6
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Appearance Solid
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분자량 427.53
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화학식 C25H33NO5
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Color White to light brown
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SMILES
O=C(OC)C1=CC=C(OC)C(NC(COC2=CC=C(C(C)(C)CC(C)(C)C)C=C2)=O)=C1
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선적
Room temperature in continental US; may vary elsewhere.
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보관
Powder -20°C 3 years In solvent -80°C 6 months -20°C 1 month
Publications (1)
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Journal Impact Factor
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Most Recent
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Cell Death Discov
α-KG inhibits tumor growth of diffuse large B-cell lymphoma by inducing ROS and TP53-mediated ferroptosis. [Abstract]2023 Jun 12;9(1):182. PMID: 37308557
MDH1-IN-2 purchased from MedChemExpress. Usage Cited in: Cell Death Discov. 2023 Jun 12;9(1):182. [Abstract]
OCI-LY1 and OCI-LY10 cells were treated with 10 μM MDH1-IN-2 pre-treatment followed by 15 mM DM-αKG. DCFDA assay was applied to detect intracellular ROS levels, represented by flow cytometry plots.
MDH1-IN-2 purchased from MedChemExpress. Usage Cited in: Cell Death Discov. 2023 Jun 12;9(1):182. [Abstract]
OCI-LY1 and OCI-LY10 cells were treated with 10 μM MDH1-IN-2 pre-treatment followed by 15 mM DM-αKG. Supernatant 2-HG levels of drug-treated cells were detected by 2-HG assay.
용액&용해도
DMSO : 25 mg/mL (58.48 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: 1 mg/mL (2.34 mM); Suspended solution; Need ultrasonic
This protocol yields a suspended solution of 1 mg/mL. Suspended solution can be used for oral and intraperitoneal injection.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (10.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: 1 mg/mL (2.34 mM); Suspended solution; Need ultrasonic
This protocol yields a suspended solution of 1 mg/mL. Suspended solution can be used for oral and intraperitoneal injection.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (10.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
순도&문서
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Data Sheet (271 KB)
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SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
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Handling Instructions (2659 KB)
References
[1]. Naik R, et al. Methyl 3-(3-(4-(2,4,4-Trimethylpentan-2-yl)phenoxy)-propanamido)benzoate as a Novel and Dual Malate Dehydrogenase (MDH) 1/2 Inhibitor Targeting Cancer Metabolism. J Med Chem. 2017;60(20):8631-8646. [Content Brief]
[2]. Cai Y, et al. α-KG inhibits tumor growth of diffuse large B-cell lymphoma by inducing ROS and TP53-mediated ferroptosis. Cell Death Discov. 2023 Jun 12;9(1):182. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 2.3390 mL | 11.6951 mL | 23.3902 mL | 58.4754 mL |
| 5 mM | 0.4678 mL | 2.3390 mL | 4.6780 mL | 11.6951 mL | |
| 10 mM | 0.2339 mL | 1.1695 mL | 2.3390 mL | 5.8475 mL | |
| 15 mM | 0.1559 mL | 0.7797 mL | 1.5593 mL | 3.8984 mL | |
| 20 mM | 0.1170 mL | 0.5848 mL | 1.1695 mL | 2.9238 mL | |
| 25 mM | 0.0936 mL | 0.4678 mL | 0.9356 mL | 2.3390 mL | |
| 30 mM | 0.0780 mL | 0.3898 mL | 0.7797 mL | 1.9492 mL | |
| 40 mM | 0.0585 mL | 0.2924 mL | 0.5848 mL | 1.4619 mL | |
| 50 mM | 0.0468 mL | 0.2339 mL | 0.4678 mL | 1.1695 mL |
MDH1-IN-2 Related Classifications
HY-147791 Related Classifications
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