2. Metabolic Enzyme/Protease Others Autophagy Immunology/Inflammation NF-κB
  3. Endogenous Metabolite Drug Derivative Autophagy Mitochondrial Metabolism Reactive Oxygen Species (ROS)
  4. Dimethyl 2-oxoglutarate

Dimethyl 2-oxoglutarate  (Synonyms: Dimethyl α-ketoglutarate)

Cat. No.: HY-44134 Purity: 97.50%
COA
SDS
Handling Instructions Technical Support

Dimethyl 2-oxoglutarate (Dimethyl α-ketoglutarate) is a cell-permeable derivative of 2-oxoglutarate and tricarboxylic acid cycle metabolite with antioxidant properties. Dimethyl 2-oxoglutarate inhibits Autophagy. Dimethyl 2-oxoglutarate prevents mitochondrial damage and reduces ROS production. Dimethyl 2-oxoglutarate alleviates Carbon tetrachloride (HY-Y0298)-induced liver fibrosis. Dimethyl-2-oxoglutaric acid can be used in the research of diseases such as Alzheimer's disease, diabetes, and cardiomyopathy.

For research use only. We do not sell to patients.

CAS No. : 13192-04-6

Size Price Stock Quantity
Liquid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
ready for reconstitution
 In-stock
Solution
10 mM * 1 mL in DMSO In-stock
Liquid
5 g In-stock
10 g In-stock
50 g   Get quote  

* Please select Quantity before adding items.

This product is a controlled substance and not for sale in your territory.

Customer Review

Based on 1 publication(s) in Google Scholar

Other Forms of Dimethyl 2-oxoglutarate:

Dimethyl 2-oxoglutarate Related Antibodies

Top Publications Citing Use of Products

1 Publications Citing Use of MCE Dimethyl 2-oxoglutarate

WB

    Dimethyl 2-oxoglutarate purchased from MedChemExpress. Usage Cited in: Commun Biol. 2025 Oct 20;8(1):1485.  [Abstract]

    Succinylation levels of ectopically expressed ME2 in HEK293T cells were measured. Cells were treated with 3-NPA (3 mM), DES (20 mM), or glycine (100 mM) for 24 h.

    View All Endogenous Metabolite Isoform Specific Products:

    View All Isoforms
    Human Endogenous Metabolite Microbial Metabolite

    • Biological Activity

    • Purity & Documentation

    • References

    • Customer Review

    Description

    Dimethyl 2-oxoglutarate (Dimethyl α-ketoglutarate) is a cell-permeable derivative of 2-oxoglutarate and tricarboxylic acid cycle metabolite with antioxidant properties. Dimethyl 2-oxoglutarate inhibits Autophagy. Dimethyl 2-oxoglutarate prevents mitochondrial damage and reduces ROS production. Dimethyl 2-oxoglutarate alleviates Carbon tetrachloride (HY-Y0298)-induced liver fibrosis. Dimethyl-2-oxoglutaric acid can be used in the research of diseases such as Alzheimer's disease, diabetes, and cardiomyopathy[1][2][3][4].

    IC50 & Target

    Microbial Metabolite

     

    In Vitro

    Dimethyl 2-oxoglutarate (1 mM; 16 h) significantly increases GLUT1 mRNA expression in diabetic muscle progenitor cells (D-MPs) and decreased glycolytic activity in non-diabetic muscle progenitor cells (ND-MPs)[1].
    Dimethyl 2-oxoglutarate replenishes cytoplasmic AcCoA levels in cultured human cells through the action of mitochondrial IDH2 or cytoplasmic IDH1[2].
    Dimethyl 2-oxoglutarate (4-16 mM; 12 h) reduces CCl4-induced liver fibrosis through inhibition of autophagy in hepatic stellate cells[3].
    Dimethyl 2-oxoglutarate (5 mM; 48 h) prevents cell death and mitochondrial damage induced by DRB18 (HY-145963) in SH-SY5Y cells[4].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    Dimethyl 2-oxoglutarate Related Antibodies
    In Vivo

    Dimethyl 2-oxoglutarate (i.p.; daily) can reduce maladaptive autophagy in the mouse thoracic aortic constriction (TAC) model, improve TAC-induced pathological remodeling, ventricular hypertrophy, and fibrosis, and reduce contractile dysfunction[2].
    Dimethyl 2-oxoglutarate (200 mg/kg; i.p; twice a week; 9 weeks) reduces CCl4-induced liver fibrosis in rats[3].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    Animal Model: Rat liver fibrosis model[3]
    Dosage: 200 mg/kg
    Administration: Intraperitoneal injection (i.p.); twice a week; 9 weeks
    Result: Decreased collagen deposition.
    Showed a 44% down-regulation of Sirius Red-stained fibrotic areas.
    Showed a slight but not significant improvement in liver function.
    Molecular Weight

    174.15

    Formula

    C7H10O5
    CAS No.
    Appearance

    Liquid (Density: 1.175±0.06 g/cm3)

    Color

    Colorless to light yellow

    SMILES

    O=C(OC)C(CCC(OC)=O)=O
    Initial Source
    Shipping

    Room temperature in continental US; may vary elsewhere.
    Storage
    Pure form -20°C 3 years
    4°C 2 years
    In solvent -80°C 6 months
    -20°C 1 month
    Solvent & Solubility
    In Vitro: 

    DMSO : 100 mg/mL (574.22 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

    H2O : 100 mg/mL (574.22 mM; Need ultrasonic)

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 5.7422 mL 28.7109 mL 57.4218 mL
    5 mM 1.1484 mL 5.7422 mL 11.4844 mL
    View the Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    * Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

    • Molarity Calculator

    • Dilution Calculator

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

    Mass
    =
    Concentration
    ×
    Volume
    ×
    Molecular Weight *

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    This equation is commonly abbreviated as: C1V1 = C2V2

    Concentration (start)

    C1

    ×
    Volume (start)

    V1

    =
    Concentration (final)

    C2

    ×
    Volume (final)

    V2

    In Vivo:

    Select the appropriate dissolution method based on your experimental animal and administration route.

    For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
    To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
    The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

    • Protocol 1

      Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

      Solubility: ≥ 2.5 mg/mL (14.36 mM); Clear solution

      This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

      Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

      Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
    • Protocol 2

      Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

      Solubility: ≥ 2.5 mg/mL (14.36 mM); Clear solution

      This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

      Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

      Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.

    For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
    The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

    • Protocol 1

      Add each solvent one by one:  PBS

      Solubility: 100 mg/mL (574.22 mM); Clear solution; Need ultrasonic

    • Protocol 2

      Add each solvent one by one:  0.5% CMC/saline water

      Solubility: ≥ 50 mg/mL (287.11 mM); Clear solution

    In Vivo Dissolution Calculator
    Please enter the basic information of animal experiments:

    Dosage

    mg/kg

    Animal weight
    (per animal)

    g

    Dosing volume
    (per animal)

    μL

    Number of animals

    Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
    Calculation results:
    Working solution concentration: mg/mL
    This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
    The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).
    Purity & Documentation

    Purity: 97.50%

    SDS (396 KB)

    COA (244 KB) HNMR (232 KB) MS (71 KB) GC (162 KB)

    Handling Instructions (2659 KB)
    References

    Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
    DMSO / H2O 1 mM 5.7422 mL 28.7109 mL 57.4218 mL 143.5544 mL
    5 mM 1.1484 mL 5.7422 mL 11.4844 mL 28.7109 mL
    10 mM 0.5742 mL 2.8711 mL 5.7422 mL 14.3554 mL
    15 mM 0.3828 mL 1.9141 mL 3.8281 mL 9.5703 mL
    20 mM 0.2871 mL 1.4355 mL 2.8711 mL 7.1777 mL
    25 mM 0.2297 mL 1.1484 mL 2.2969 mL 5.7422 mL
    30 mM 0.1914 mL 0.9570 mL 1.9141 mL 4.7851 mL
    40 mM 0.1436 mL 0.7178 mL 1.4355 mL 3.5889 mL
    50 mM 0.1148 mL 0.5742 mL 1.1484 mL 2.8711 mL
    60 mM 0.0957 mL 0.4785 mL 0.9570 mL 2.3926 mL
    80 mM 0.0718 mL 0.3589 mL 0.7178 mL 1.7944 mL
    100 mM 0.0574 mL 0.2871 mL 0.5742 mL 1.4355 mL

    * Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

    • No file chosen (Maximum size is: 1024 Kb)
    • If you have published this work, please enter the PubMed ID.
    • Your name will appear on the site.

    Dimethyl 2-oxoglutarate Related Classifications

    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    Dimethyl 2-oxoglutarate13192-04-6Dimethyl α-ketoglutarateEndogenous MetaboliteDrug DerivativeAutophagyMitochondrial MetabolismReactive Oxygen Species (ROS)Drug derivativeAlzheimer's diseasediabetescardiomyopathyInhibitorinhibitorinhibit

    Your Recently Viewed Products:

    Inquiry Online

    Your information is safe with us. * Required Fields.

    Product Name

    		  

    Requested Quantity *

    Applicant Name *

    		 

    Salutation

    Email Address *

    		 

    Phone Number *

    Department

    		 

    Organization Name *

    City

    State

    Country or Region *

    		      

    Remarks

    Bulk Inquiry

    Inquiry Information

    Product Name:
    Dimethyl 2-oxoglutarate
    Cat. No.:
    HY-44134
    Quantity:
    MCE Japan Authorized Agent:

    Customer Review

    Thank You for Your Feedback!

    Request for HNMR Report

    Please fill out this form to request the QC report. We will send it to your Email address shortly.

    Your information is safe with us. * Required Fields.

    Product Name

    		  

    Lot #

    Applicant Name *

    		 

    Email Address *

    Phone Number

    		 

    Department *

    Organization Name *

    		 

    Country or Region *

    Remarks

    SAFETY DATA SHEET (SDS)

    English - EN (396 KB) Français - FR (396 KB) Deutsch - DE (396 KB) Norwegian - NO (396 KB) Español - ES (396 KB) Swedish - SV (396 KB) Italian - IT (396 KB) Korean - KR (396 KB) Portuguese - PT (396 KB)

    Request for HNMR Report

    We have received your request and will respond to you as soon as possible.