Cat. No.: HY-44134

Dimethyl 2-oxoglutarate

(Synonyms: Dimethyl α-ketoglutarate)

3 Cited Publications

Customer Review

Based on 3 publication(s) in Google Scholar

Dimethyl 2-oxoglutarate (Dimethyl α-ketoglutarate) is a cell-permeable derivative of 2-oxoglutarate and tricarboxylic acid cycle metabolite with antioxidant properties. Dimethyl 2-oxoglutarate inhibits Autophagy. Dimethyl 2-oxoglutarate prevents mitochondrial damage and reduces ROS production. Dimethyl 2-oxoglutarate alleviates Carbon tetrachloride (HY-Y0298)-induced liver fibrosis. Dimethyl-2-oxoglutaric acid can be used in the research of diseases such as Alzheimer's disease, diabetes, and cardiomyopathy.

For research use only. We do not sell to patients.

Purity: 97.50%
CAS No.: 13192-04-6
Formula: C₇H₁₀O₅
Molecular Weight:174.15

Storage:

Pure form -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month

Dimethyl 2-oxoglutarate Chemical Structure

CAS No.: 13192-04-6

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Use of MCE Products

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Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biological Activity
- Description
- IC₅₀ & Target
- In Vitro
- In Vivo
Chemical Information
Publications (3)
Solvent & Solubility
- In Vitro
- In Vivo
Purity & Documentation
References
Help & FAQs
Classifications
Bioz

Biological Activity

Description

IC₅₀ & Target

Microbial Metabolite

All Endogenous Metabolite Isoforms :

In Vitro

Dimethyl 2-oxoglutarate (1 mM; 16 h) significantly increases GLUT1 mRNA expression in diabetic muscle progenitor cells (D-MPs) and decreased glycolytic activity in non-diabetic muscle progenitor cells (ND-MPs)^[1].
Dimethyl 2-oxoglutarate replenishes cytoplasmic AcCoA levels in cultured human cells through the action of mitochondrial IDH2 or cytoplasmic IDH1^[2].
Dimethyl 2-oxoglutarate (4-16 mM; 12 h) reduces CCl₄-induced liver fibrosis through inhibition of autophagy in hepatic stellate cells^[3].
Dimethyl 2-oxoglutarate (5 mM; 48 h) prevents cell death and mitochondrial damage induced by DRB18 (HY-145963) in SH-SY5Y cells^[4].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

In Vivo

Dimethyl 2-oxoglutarate (i.p.; daily) can reduce maladaptive autophagy in the mouse thoracic aortic constriction (TAC) model, improve TAC-induced pathological remodeling, ventricular hypertrophy, and fibrosis, and reduce contractile dysfunction^[2].
Dimethyl 2-oxoglutarate (200 mg/kg; i.p; twice a week; 9 weeks) reduces CCl₄-induced liver fibrosis in rats^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:

Rat liver fibrosis model^[3]
Dosage:

200 mg/kg
Administration:

Intraperitoneal injection (i.p.); twice a week; 9 weeks
Result:

Decreased collagen deposition.
Showed a 44% down-regulation of Sirius Red-stained fibrotic areas.
Showed a slight but not significant improvement in liver function.

Chemical Information

CAS No. 13192-04-6
Appearance Liquid (Density: 1.175±0.06 g/cm³)
Molecular Weight 174.15
Formula C₇H₁₀O₅
Color Colorless to light yellow
SMILES

O=C(OC)C(CCC(OC)=O)=O
Synonyms

Dimethyl α-ketoglutarate
Initial Source
- Endogenous metabolite
Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Pure form	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

Publications (3)

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Sci Bull

Hepatic IFRD1 suppresses metabolic dysfunction-associated fatty liver disease via GLUD1/α-KG axis. [Abstract]

2026 May 15;71(9):2283-2299. PMID: 41997855
Cell Rep

4-octyl itaconate inhibits cytokine-mediated inflammation via alkylation of TYK2 and JAK1. [Abstract]

2026 Mar 30;45(4):117179. PMID: 41915469
Commun Biol

SIRT5-mediated desuccinylation of MTHFD2 enhances chemoresistance in breast cancer cells by reducing therapy-induced senescence. [Abstract]

2025 Oct 20;8(1):1485. PMID: 41116031

Dimethyl 2-oxoglutarate purchased from MedChemExpress. Usage Cited in: Commun Biol. 2025 Oct 20;8(1):1485. [Abstract]

Succinylation levels of ectopically expressed ME2 in HEK293T cells were measured. Cells were treated with 3-NPA (3 mM), DES (20 mM), or glycine (100 mM) for 24 h.

Solvent & Solubility

In Vitro:

DMSO : 100 mg/mL (574.22 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

H₂O : 100 mg/mL (574.22 mM; Need ultrasonic)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

5.7422 mL

28.7109 mL

57.4218 mL

5 mM

1.1484 mL

5.7422 mL

11.4844 mL

10 mM

0.5742 mL

2.8711 mL

5.7422 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Molarity Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Concentration Concentration

Volume Volume

Molecular Weight Molecular Weight

Dilution Calculator

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

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Volume _(start) Volume _(start)

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In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

Stock Solution Working Solution

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline

Solubility: ≥ 2.5 mg/mL (14.36 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)

Solubility: ≥ 2.5 mg/mL (14.36 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil

Solubility: ≥ 2.5 mg/mL (14.36 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

Working Solution

For the following dissolution methods, please prepare the working solution directly:

It is recommended to prepare fresh solutions and use them promptly within a short period of time.

The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: PBS

Solubility: 100 mg/mL (574.22 mM); Clear solution; Need ultrasonic

Protocol 2

Add each solvent one by one: 0.5% CMC/saline water

Solubility: ≥ 50 mg/mL (287.11 mM); Clear solution

Protocol 3

Add each solvent one by one: 50% PEG300 50% PBS

Solubility: ≥ 50 mg/mL (287.11 mM); Clear solution

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage Dosage
mg/kg
Animal Weight (Per Animal) Animal Weight (Per Animal)
g
Dosing Volume (Per Animal) Dosing Volume (Per Animal)
μL
Number of Animals Number of Animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Working solution concentration: 0.22 mg/mL

This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact

MedChemExpress (MCE) .

Purity & Documentation

Select Batch:

Purity: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (276 KB)
SDS (396 KB)
- English - EN (396 KB)
- Français - FR (396 KB)
- Deutsch - DE (396 KB)
- Norwegian - NO (396 KB)
- Español - ES (396 KB)
- Swedish - SV (396 KB)
- Italian - IT (396 KB)
- Korean - KR (396 KB)
- Portuguese - PT (396 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

References

[1]. Faulkner A, et al. Dimethyl-2-oxoglutarate improves redox balance and mitochondrial function in muscle pericytes of individuals with diabetes mellitus. Diabetologia. 2020 Oct;63(10):2205-2217. [Content Brief]

[2]. Mariño G, et al. Dimethyl α-ketoglutarate inhibits maladaptive autophagy in pressure overload-induced cardiomyopathy. Autophagy. 2014 May;10(5):930-2. [Content Brief]

[3]. Zhao J, et al. Dimethyl α-ketoglutarate reduces CCl4-induced liver fibrosis through inhibition of autophagy in hepatic stellate cells. Biochem Biophys Res Commun. 2016 Dec 2;481(1-2):90-96. [Content Brief]

[4]. Chauhan A, et al. Dimethyl-2-oxoglutarate but not antioxidants prevents glucose hypometabolism induced neural cell death: implications in the pathogenesis and therapy of Alzheimer's disease. Biochem Biophys Rep. 2025 Jul 12;43:102150. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
DMSO / H₂O	1 mM	5.7422 mL	28.7109 mL	57.4218 mL	143.5544 mL
	5 mM	1.1484 mL	5.7422 mL	11.4844 mL	28.7109 mL
	10 mM	0.5742 mL	2.8711 mL	5.7422 mL	14.3554 mL
	15 mM	0.3828 mL	1.9141 mL	3.8281 mL	9.5703 mL
	20 mM	0.2871 mL	1.4355 mL	2.8711 mL	7.1777 mL
	25 mM	0.2297 mL	1.1484 mL	2.2969 mL	5.7422 mL
	30 mM	0.1914 mL	0.9570 mL	1.9141 mL	4.7851 mL
	40 mM	0.1436 mL	0.7178 mL	1.4355 mL	3.5889 mL
	50 mM	0.1148 mL	0.5742 mL	1.1484 mL	2.8711 mL
	60 mM	0.0957 mL	0.4785 mL	0.9570 mL	2.3926 mL
	80 mM	0.0718 mL	0.3589 mL	0.7178 mL	1.7944 mL
	100 mM	0.0574 mL	0.2871 mL	0.5742 mL	1.4355 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Keywords

Dimethyl 2-oxoglutarate

3 Cited Publications

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Dimethyl 2-oxoglutarate

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Publications Citing Use of MedChemExpress (MCE) Dimethyl 2-oxoglutarate

All Endogenous Metabolite Isoforms

Biological Activity

Chemical Information

Publications (3)

Solvent & Solubility

Purity & Documentation

References

Help & FAQs

Complete Stock Solution Preparation Table

Dimethyl 2-oxoglutarate Related Classifications

HY-44134 Related Classifications