Dimethyl 2-oxoglutarate
Based on 6 publication(s) in Google Scholar
Dimethyl 2-oxoglutarate (Dimethyl α-ketoglutarate) is a cell-permeable derivative of 2-oxoglutarate and tricarboxylic acid cycle metabolite with antioxidant properties. Dimethyl 2-oxoglutarate inhibits Autophagy. Dimethyl 2-oxoglutarate prevents mitochondrial damage and reduces ROS production. Dimethyl 2-oxoglutarate alleviates Carbon tetrachloride (HY-Y0298)-induced liver fibrosis. Dimethyl-2-oxoglutaric acid can be used in the research of diseases such as Alzheimer's disease, diabetes, and cardiomyopathy.
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- Purity : 97.50%
- CAS No.: 13192-04-6
- 화학식: C7H10O5
- 분자량:174.15
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보관:Pure form -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month
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Biological Activity
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Chemical Information
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용액&용해도
- Protocol
- 순도&문서
- References
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Publications Citing Use of MedChemExpress (MCE) Dimethyl 2-oxoglutarate
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All Endogenous Metabolite Isoforms
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Biological Activity
제품 설명
IC50 & Target
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Microbial Metabolite |
In Vitro
Dimethyl 2-oxoglutarate (1 mM; 16 h) significantly increases GLUT1 mRNA expression in diabetic muscle progenitor cells (D-MPs) and decreased glycolytic activity in non-diabetic muscle progenitor cells (ND-MPs)[1].
Dimethyl 2-oxoglutarate replenishes cytoplasmic AcCoA levels in cultured human cells through the action of mitochondrial IDH2 or cytoplasmic IDH1[2].
Dimethyl 2-oxoglutarate (4-16 mM; 12 h) reduces CCl4-induced liver fibrosis through inhibition of autophagy in hepatic stellate cells[3].
Dimethyl 2-oxoglutarate (5 mM; 48 h) prevents cell death and mitochondrial damage induced by DRB18 (HY-145963) in SH-SY5Y cells[4].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
In Vivo
Dimethyl 2-oxoglutarate (200 mg/kg; i.p; twice a week; 9 weeks) reduces CCl4-induced liver fibrosis in rats[3].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:Rat liver fibrosis model[3]
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Dosage:200 mg/kg
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Administration:Intraperitoneal injection (i.p.); twice a week; 9 weeks
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Result:Decreased collagen deposition.
Showed a 44% down-regulation of Sirius Red-stained fibrotic areas.
Showed a slight but not significant improvement in liver function.
- Fibrosis/Collagen Morphometry
- Collagen: Sirius Red Staining
- ROS/oxidative-stress fluorescent staining
- Connective Tissue: Masson's Trichrome/Collagen Trichrome Staining
- Autophagy
- Lysosome and acidic-vesicle live-cell staining
- Macroautophagy Solutions
- Research Protocol for Metabolic Diseases
- Liver Histomorphometry
- Alzheimer’s Disease Modeling
Chemical Information
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CAS No. 13192-04-6
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Appearance Liquid (Density: 1.175±0.06 g/cm3)
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분자량 174.15
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화학식 C7H10O5
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Color Colorless to light yellow
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SMILES
O=C(OC)C(CCC(OC)=O)=O
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Synonyms
Dimethyl α-ketoglutarate
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Initial Source
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선적
Room temperature in continental US; may vary elsewhere.
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보관
Pure form -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Publications (6)
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Journal Impact Factor
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Most Recent
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Sci Bull
Hepatic IFRD1 suppresses metabolic dysfunction-associated fatty liver disease via GLUD1/α-KG axis. [Abstract]2026 May 15;71(9):2283-2299. PMID: 41997855 -
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Cell Rep
4-octyl itaconate inhibits cytokine-mediated inflammation via alkylation of TYK2 and JAK1. [Abstract]2026 Mar 30;45(4):117179. PMID: 41915469 -
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Commun Biol
SIRT5-mediated desuccinylation of MTHFD2 enhances chemoresistance in breast cancer cells by reducing therapy-induced senescence. [Abstract]2025 Oct 20;8(1):1485. PMID: 41116031
Dimethyl 2-oxoglutarate purchased from MedChemExpress. Usage Cited in: Commun Biol. 2025 Oct 20;8(1):1485. [Abstract]
Succinylation levels of ectopically expressed ME2 in HEK293T cells were measured. Cells were treated with 3-NPA (3 mM), DES (20 mM), or glycine (100 mM) for 24 h.
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용액&용해도
In Vitro:
DMSO : 100 mg/mL (574.22 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
H2O : 100 mg/mL (574.22 mM; Need ultrasonic)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
In Vivo:
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (14.36 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (14.36 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
For the following dissolution methods, please prepare the working solution directly:
It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: PBS
Solubility: 100 mg/mL (574.22 mM); Clear solution; Need ultrasonic
Add each solvent one by one: 50% PEG300 50% PBS
Solubility: ≥ 50 mg/mL (287.11 mM); Clear solution
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Working solution concentration: 0.22 mg/mL
This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
순도&문서
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Data Sheet (278 KB)
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SDS (396 KB)
- English - EN (396 KB)
- Français - FR (396 KB)
- Deutsch - DE (396 KB)
- Norwegian - NO (396 KB)
- Español - ES (396 KB)
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Handling Instructions (2659 KB)
References
[1]. Faulkner A, et al. Dimethyl-2-oxoglutarate improves redox balance and mitochondrial function in muscle pericytes of individuals with diabetes mellitus. Diabetologia. 2020 Oct;63(10):2205-2217. [Content Brief]
[2]. Mariño G, et al. Dimethyl α-ketoglutarate inhibits maladaptive autophagy in pressure overload-induced cardiomyopathy. Autophagy. 2014 May;10(5):930-2. [Content Brief]
[3]. Zhao J, et al. Dimethyl α-ketoglutarate reduces CCl4-induced liver fibrosis through inhibition of autophagy in hepatic stellate cells. Biochem Biophys Res Commun. 2016 Dec 2;481(1-2):90-96. [Content Brief]
[4]. Chauhan A, et al. Dimethyl-2-oxoglutarate but not antioxidants prevents glucose hypometabolism induced neural cell death: implications in the pathogenesis and therapy of Alzheimer's disease. Biochem Biophys Rep. 2025 Jul 12;43:102150. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO / H2O | 1 mM | 5.7422 mL | 28.7109 mL | 57.4218 mL | 143.5544 mL |
| 5 mM | 1.1484 mL | 5.7422 mL | 11.4844 mL | 28.7109 mL | |
| 10 mM | 0.5742 mL | 2.8711 mL | 5.7422 mL | 14.3554 mL | |
| 15 mM | 0.3828 mL | 1.9141 mL | 3.8281 mL | 9.5703 mL | |
| 20 mM | 0.2871 mL | 1.4355 mL | 2.8711 mL | 7.1777 mL | |
| 25 mM | 0.2297 mL | 1.1484 mL | 2.2969 mL | 5.7422 mL | |
| 30 mM | 0.1914 mL | 0.9570 mL | 1.9141 mL | 4.7851 mL | |
| 40 mM | 0.1436 mL | 0.7178 mL | 1.4355 mL | 3.5889 mL | |
| 50 mM | 0.1148 mL | 0.5742 mL | 1.1484 mL | 2.8711 mL | |
| 60 mM | 0.0957 mL | 0.4785 mL | 0.9570 mL | 2.3926 mL | |
| 80 mM | 0.0718 mL | 0.3589 mL | 0.7178 mL | 1.7944 mL | |
| 100 mM | 0.0574 mL | 0.2871 mL | 0.5742 mL | 1.4355 mL |
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Dimethyl 2-oxoglutarate Related Classifications
HY-44134 Related Classifications
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