2. Metabolic Enzyme/Protease
  3. Tyrosinase Glucosidase
  4. Kushenol A

Kushenol A  (Synonyms: Leachianone E)

Cat. No.: HY-N2278 Purity: 99.84%
COA Handling Instructions

Molarity Calculator

Kushenol A (Leachianone E) is isolated from the root of Sophora flavescent. Kushenol A is a non-competitive tyrosinase inhibitor to block the conversion of L-tyrosine to L-DOPA, shows IC50 and Kivalues of 1.1 μM and 0.4 μM, respectively. Kushenol A is a flavonoid antioxidant, has inhibitory effects on alpha-glucosidase (IC50: 45 μM; Ki: 6.8 μM) and β-amylase. Kushenol A is confirmed as potential inhibitors of enzymes targeted by cosmetics for skin whitening and aging.

For research use only. We do not sell to patients.

Kushenol A Chemical Structure

Kushenol A Chemical Structure

CAS No. : 99217-63-7

Size Price Stock Quantity
Solid + Solvent
10 mM * 1 mL in DMSO
ready for reconstitution
 USD 660 In-stock
Solution
10 mM * 1 mL in DMSO USD 660 In-stock
Solid
5 mg USD 600 In-stock
10 mg USD 960 In-stock
50 mg   Get quote  
100 mg   Get quote  

* Please select Quantity before adding items.

This product is a controlled substance and not for sale in your territory.

Customer Review

Based on 1 publication(s) in Google Scholar

Kushenol A Related Antibodies

Top Publications Citing Use of Products

1 Publications Citing Use of MCE Kushenol A

  • Biological Activity

  • Purity & Documentation

  • References

  • Customer Review

Description

Kushenol A (Leachianone E) is isolated from the root of Sophora flavescent. Kushenol A is a non-competitive tyrosinase inhibitor to block the conversion of L-tyrosine to L-DOPA, shows IC50 and Kivalues of 1.1 μM and 0.4 μM, respectively[1]. Kushenol A is a flavonoid antioxidant, has inhibitory effects on alpha-glucosidase (IC50: 45 μM; Ki: 6.8 μM) and β-amylase[2]. Kushenol A is confirmed as potential inhibitors of enzymes targeted by cosmetics for skin whitening and aging[1].

IC50 & Target

IC50: 1.1 μM (tyrosinase); 45 μM (alpha-glucosidase)[1][2]
In Vitro

Kushenol A (25 μM) exhibits a highly potent inhibitory activity on ABTS+ radical scavenging with an IC50 value of 9.7 ± 0.1 μM, and exhibits inhibits scavenging activity as a percentage 93.7% at 25 μM[1].
Kushenol A (0-60 μg/ml; 24 hours) shows considerable cytotoxic effects against NSCLC cells, exhibits IC50 values of 5.3 μg/ml and 20.5 μg/ml for A549 and NCI‐H226 cells, respectively. It shows an IC50 value of 57.2 μg/ml for BEAS-2B cells[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Kushenol A Related Antibodies

Cell Viability Assay[3]

Cell Line: NSCLC cell lines, A549 and NCI-H226
Normal human lung epithelial: BEAS-2B
Concentration: 0-60 μg/ml
Incubation Time: 24 hours
Result: Exhibited considerable cytotoxic effects against NSCLC cells.
Molecular Weight

408.49

Formula

C25H28O5
CAS No.
Appearance

Solid

Color

White to off-white

SMILES

O=C1C[C@@H](C2=CC=CC=C2O)OC3=C(C[C@H](C(C)=C)C/C=C(C)\C)C(O)=CC(O)=C13
Structure Classification
Initial Source
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

4°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Solvent & Solubility
In Vitro: 

DMSO : 100 mg/mL (244.80 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.4480 mL 12.2402 mL 24.4804 mL
5 mM 0.4896 mL 2.4480 mL 4.8961 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
Purity & Documentation

Purity: 99.84%

COA (252 KB) HNMR (237 KB) RP-HPLC (245 KB) MS (918 KB)

Handling Instructions (2659 KB)
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 2.4480 mL 12.2402 mL 24.4804 mL 61.2010 mL
5 mM 0.4896 mL 2.4480 mL 4.8961 mL 12.2402 mL
10 mM 0.2448 mL 1.2240 mL 2.4480 mL 6.1201 mL
15 mM 0.1632 mL 0.8160 mL 1.6320 mL 4.0801 mL
20 mM 0.1224 mL 0.6120 mL 1.2240 mL 3.0601 mL
25 mM 0.0979 mL 0.4896 mL 0.9792 mL 2.4480 mL
30 mM 0.0816 mL 0.4080 mL 0.8160 mL 2.0400 mL
40 mM 0.0612 mL 0.3060 mL 0.6120 mL 1.5300 mL
50 mM 0.0490 mL 0.2448 mL 0.4896 mL 1.2240 mL
60 mM 0.0408 mL 0.2040 mL 0.4080 mL 1.0200 mL
80 mM 0.0306 mL 0.1530 mL 0.3060 mL 0.7650 mL
100 mM 0.0245 mL 0.1224 mL 0.2448 mL 0.6120 mL
  • No file chosen (Maximum size is: 1024 Kb)
  • If you have published this work, please enter the PubMed ID.
  • Your name will appear on the site.

Kushenol A Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Kushenol A99217-63-7Leachianone ETyrosinaseGlucosidasetyrosinasealpha-glucosidaseskinwhiteningNSCLCA549NCI-H226NormalhumanlungepithelialBEAS-2BInhibitorinhibitorinhibit

Your Recently Viewed Products:

Inquiry Online

Your information is safe with us. * Required Fields.

Product Name

  

Salutation

Applicant Name *

 

Email Address *

Phone Number *

 

Organization Name *

Department *

 

Requested quantity *

Country or Region *

      

Remarks

Bulk Inquiry

Inquiry Information

Product Name:
Kushenol A
Cat. No.:
HY-N2278
Quantity:
MCE Japan Authorized Agent:

Customer Review

Thank You for Your Feedback!

Request for HNMR Report

Please fill out this form to request the QC report. We will send it to your Email address shortly.

Your information is safe with us. * Required Fields.

Product Name

  

Lot #

Applicant Name *

 

Email Address *

Phone Number

 

Department *

Organization Name *

 

Country or Region *

Remarks

Request for HNMR Report

We have received your request and will respond to you as soon as possible.