LNP Lipid-8
Based on 1 Customer Validation
LNP Lipid-8 (11-A-M) is an ionizable single-tail multi-head lipid that can be used as a lipid nanoparticle (LNP) to deliver siRNA to T cells without targeting ligands. LNP Lipid-8 is more selective for T cells than other cell types such as hepatocytes. LNP Lipid-8 selectively delivers siRNA/sgRNA to T cells (especially CD8+ T cells) through endogenous lipid transport pathways, and can enter cells and release RNA through endocytosis to achieve gene silencing. LNP Lipid-8 loaded with GFP siRNA (siGFP) significantly led to GFP gene silencing in mouse models. LNP Lipid-8 showed good efficacy and safety in both cells and animals, without obvious liver targeting and toxicity. LNP Lipid-8 can be used for RNA delivery research in the fields of tumor immunotherapy and T cell-mediated autoimmune diseases.
For research use only. We do not sell to patients.
- Purity: 95.0%
- CAS No.: 2408140-60-1
- Formula: C42H71NO7
- Molecular Weight:702.02
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Storage:Pure form -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
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Metabolism/Protease Compound Library
HY-L012
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Anti-Cancer Compound Library
HY-L025
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Lipid Compound Library
HY-L043
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Bioactive Compound Library Max
HY-L181
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MCE Bioactive Compound Library
HY-L001V
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Bioactive Compound Library
HY-L001
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High-Throughput Bioactive Compound Library
HY-L205
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Liposome Library
HY-L214
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Ionizable Lipid Compound Library
HY-L251
Biological Activity
LNP Lipid-8 can encapsulate approximately 75% of siRNA/sgRNA in vitro, and transmission electron microscopy shows a spherical structure[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
LNP Lipid-8 (0.5 mg/kg; intravenous injection; single dose; 3 days) loaded with siGFP, achieves T cell gene silencing in a GFP transgenic C57BL/6 mouse model, with the lowest effective dose of 0.5 mg/kg and no obvious liver toxicity[1].
LNP Lipid-8 (2.0 mg/kg; intravenous injection; single dose; 5 days) loaded with siGFP, delivered sgRNA in a Cas9-GFP transgenic mouse model, inducing GFP gene editing in CD8+ T cells, with editing efficiency significantly higher than that in CD4+ T cells[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 2408140-60-1
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Appearance Liquid (Density: 1.034±0.06 g/cm3)
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Molecular Weight 702.02
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Formula C42H71NO7
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Color Colorless to light yellow
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SMILES
CCN(CC)CCCOC(OCC(COC(CCCCCCC/C=C\C/C=C\CCCCC)=O)COC(C[C@@]12CC3C[C@H](C[C@@H](C3)C2)C1)=O)=O
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Pure form -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Solvent & Solubility
DMSO : ≥ 100 mg/mL (142.45 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
* "≥" means soluble, but saturation unknown.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (3.56 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: 2.5 mg/mL (3.56 mM); Suspended solution; Need ultrasonic
This protocol yields a suspended solution of 2.5 mg/mL. Suspended solution can be used for oral and intraperitoneal injection.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
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Data Sheet (268 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 1.4245 mL | 7.1223 mL | 14.2446 mL | 35.6115 mL |
| 5 mM | 0.2849 mL | 1.4245 mL | 2.8489 mL | 7.1223 mL | |
| 10 mM | 0.1424 mL | 0.7122 mL | 1.4245 mL | 3.5612 mL | |
| 15 mM | 0.0950 mL | 0.4748 mL | 0.9496 mL | 2.3741 mL | |
| 20 mM | 0.0712 mL | 0.3561 mL | 0.7122 mL | 1.7806 mL | |
| 25 mM | 0.0570 mL | 0.2849 mL | 0.5698 mL | 1.4245 mL | |
| 30 mM | 0.0475 mL | 0.2374 mL | 0.4748 mL | 1.1871 mL | |
| 40 mM | 0.0356 mL | 0.1781 mL | 0.3561 mL | 0.8903 mL | |
| 50 mM | 0.0285 mL | 0.1424 mL | 0.2849 mL | 0.7122 mL | |
| 60 mM | 0.0237 mL | 0.1187 mL | 0.2374 mL | 0.5935 mL | |
| 80 mM | 0.0178 mL | 0.0890 mL | 0.1781 mL | 0.4451 mL | |
| 100 mM | 0.0142 mL | 0.0712 mL | 0.1424 mL | 0.3561 mL |
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