Referencia número: HY-136538

LQZ-7I

Name: LQZ-7I
Brand: MedChemExpress
SKU: HY-136538
Price: 60.00 USD
Availability: InStock
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LQZ-7I is a survivin-targeting inhibitor. LQZ-7I inhibits survivin dimerization. LQZ-7I orally effectively inhibits xenograft tumor growth and induces survivin loss in tumors.

Para uso exclusivo en investigación. No vendemos a pacientes.

Pureza : 99.78%
No. CAS: 195822-23-2
Fòrmula: C₂₀H₁₄F₂N₄
Peso molecular:348.35

Almacenamiento:

Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 2 years , -20°C, 1 year

LQZ-7I Chemical Structure

No. CAS: 195822-23-2

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Top Publications Citing

Use of MCE Products

Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Actividad biológica
- Descripciòn
- IC₅₀ & Target
- Cellular Effect
- In Vitro
- In Vivo
Chemical Information
Solvente y solubilidad
- In Vitro
- In Vivo
Pureza y Documentación
Referencias
Help & FAQs
Classifications
Bioz

Actividad biológica

Descripciòn

LQZ-7I is a survivin-targeting inhibitor. LQZ-7I inhibits survivin dimerization. LQZ-7I orally effectively inhibits xenograft tumor growth and induces survivin loss in tumors^[1].

IC₅₀ & Target

Survivin^[1]

Cellular Effect

Cell Line	Type	Value	Description	References
LNCaP C4-2	IC₅₀	2 μM Compound: LQZ-7I; 7I	Cytotoxicity against human C4-2 cells transfected with pcDNA3.1 assessed as reduction in cell viability measured after 72 hrs by methylene blue staining based assay Cytotoxicity against human C4-2 cells transfected with pcDNA3.1 assessed as reduction in cell viability measured after 72 hrs by methylene blue staining based assay	[PMID: 32421328]
LNCaP C4-2	IC₅₀	3.1 μM Compound: LQZ-7I; 7I	Cytotoxicity against human C4-2 cells assessed as reduction in cell viability measured after 72 hrs by methylene blue staining based assay Cytotoxicity against human C4-2 cells assessed as reduction in cell viability measured after 72 hrs by methylene blue staining based assay	[PMID: 32421328]
LNCaP C4-2	IC₅₀	3.1 μM Compound: 9; LQZ-7I	Cytotoxicity against human LNCaP C4-2 cells assessed as cell growth inhibition incubated for 24 hrs by MTT assay Cytotoxicity against human LNCaP C4-2 cells assessed as cell growth inhibition incubated for 24 hrs by MTT assay	[PMID: 38092421]
LNCaP C4-2	IC₅₀	7 μM Compound: LQZ-7I; 7I	Cytotoxicity against human C4-2 cells transfected with HA-tagged survivin assessed as reduction in cell viability measured after 72 hrs by methylene blue staining based assay Cytotoxicity against human C4-2 cells transfected with HA-tagged survivin assessed as reduction in cell viability measured after 72 hrs by methylene blue staining based assay	[PMID: 32421328]
PC-3	IC₅₀	4.8 μM Compound: LQZ-7I; 7I	Cytotoxicity against human PC3 cells assessed as reduction in cell viability measured after 72 hrs by methylene blue staining based assay Cytotoxicity against human PC3 cells assessed as reduction in cell viability measured after 72 hrs by methylene blue staining based assay	[PMID: 32421328]
PC-3	IC₅₀	4.8 μM Compound: 9; LQZ-7I	Cytotoxicity against human PC-3 cells assessed as reduction in cell viability incubated for 24 hrs by MTT assay Cytotoxicity against human PC-3 cells assessed as reduction in cell viability incubated for 24 hrs by MTT assay	[PMID: 38092421]

In Vitro

LQZ-7I has improved cytotoxicity with IC₅₀s of 3.1 μM against C4-2 cells and 4.8 μM against PC-3 cells compared with the parent compound LQZ-7^[1].
? LQZ-7I (10 μM; 0-6 hours) treatment reduces the expression of survivin. However, LQZ-7I does not reduce the expression of XIAP, CIAP1, and CIAP2. LQZ-7I may be selective to its intended target survivin^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Western Blot Analysis^[1]

Cell Line:

PC-3 or C4-2 cells
Concentration:

10 µM
Incubation Time:

0-6 hours
Result:

Reduced the expression of survivin.

In Vivo

LQZ-7I (100 mg/kg; oral gavage every other day for a total of ten treatments) significantly suppresses tumor growth without any notable adverse effect on the mice^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:

6-week old male NSG mice^[1]
Dosage:

100 mg/kg; 200 µL vehicle (90% corn oil/10% DMSO)
Administration:

Oral gavage every other day for a total of ten treatments
Result:

Significantly suppressed tumor growth without any notable adverse effect on the mice as indicated by lacking changes in body weight and in wet weight of major organs at the end of the study.

Related Protocols :

Chemical Information

No. CAS 195822-23-2
Appearance Solid
Peso molecular 348.35
Fòrmula C₂₀H₁₄F₂N₄
Color Light yellow to green yellow
SMILES

FC1=CC=C(NC2=NC3=CC=CC=C3N=C2NC4=CC=C(F)C=C4)C=C1
Envío

Room temperature in continental US; may vary elsewhere.

Almacenamiento

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	2 years
	-20°C	1 year

Solvente y solubilidad

In Vitro:

DMSO : 125 mg/mL (358.83 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

2.8707 mL

14.3534 mL

28.7068 mL

5 mM

0.5741 mL

2.8707 mL

5.7414 mL

10 mM

0.2871 mL

1.4353 mL

2.8707 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Calculadora de molaridad

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Concentration Concentration

Volume Volume

Molecular Weight Molecular Weight

Calculadora de dilución

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

Concentration _(start) Concentration _(start)

Volume _(start) Volume _(start)

Concentration _(final) Concentration _(final)

Volume _(final) Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

Stock Solution Working Solution

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 90% Corn Oil

Solubility: ≥ 2.08 mg/mL (5.97 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage Dosage
mg/kg
Animal Weight (Per Animal) Animal Weight (Per Animal)
g
Dosing Volume (Per Animal) Dosing Volume (Per Animal)
μL
Number of Animals Number of Animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

%
DMSO +

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
%
+
+
%
Tween-80 + +
%
Saline +

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Working solution concentration: 0.22 mg/mL

Pureza y Documentación

Select Batch:

Pureza: 99.98%

Assay: 99.98%

ee.: 99.98%

Ficha de datos (276 KB)
SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Instrucciones de manejo (2659 KB)

Referencias

[1]. Robert Peery,et al. Synthesis and Identification of a Novel Lead Targeting Survivin Dimerization for Proteasome-Dependent Degradation. J Med Chem. 2020 Jun 9. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
DMSO	1 mM	2.8707 mL	14.3534 mL	28.7068 mL	71.7669 mL
	5 mM	0.5741 mL	2.8707 mL	5.7414 mL	14.3534 mL
	10 mM	0.2871 mL	1.4353 mL	2.8707 mL	7.1767 mL
	15 mM	0.1914 mL	0.9569 mL	1.9138 mL	4.7845 mL
	20 mM	0.1435 mL	0.7177 mL	1.4353 mL	3.5883 mL
	25 mM	0.1148 mL	0.5741 mL	1.1483 mL	2.8707 mL
	30 mM	0.0957 mL	0.4784 mL	0.9569 mL	2.3922 mL
	40 mM	0.0718 mL	0.3588 mL	0.7177 mL	1.7942 mL
	50 mM	0.0574 mL	0.2871 mL	0.5741 mL	1.4353 mL
	60 mM	0.0478 mL	0.2392 mL	0.4784 mL	1.1961 mL
	80 mM	0.0359 mL	0.1794 mL	0.3588 mL	0.8971 mL
	100 mM	0.0287 mL	0.1435 mL	0.2871 mL	0.7177 mL

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LQZ-7I