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  4. M1219

M1219 is a GSH/ATP dual near-infrared activated fluorescent probe that enables independent real-time monitoring of dynamic changes in intracellular GSH and ATP without spectral crosstalk (GSH: Ex=640 nm, Em=740~800 nm; ATP: Ex=594 nm/610 nm, Em=650~700 nm). M1219 not only visualizes the metabolic regulatory mechanism of TNBC under single/dual-target inhibition of SLC7A11/GLUT1 and accurately evaluates its in vivo efficacy, but also achieves precise localization of the TNBC tumor invasion boundary. M1219 can be used for the research of triple-negative breast cancer.

For research use only. We do not sell to patients.

M1219

M1219 Chemical Structure

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Description

M1219 is a GSH/ATP dual near-infrared activated fluorescent probe that enables independent real-time monitoring of dynamic changes in intracellular GSH and ATP without spectral crosstalk (GSH: Ex=640 nm, Em=740~800 nm; ATP: Ex=594 nm/610 nm, Em=650~700 nm). M1219 not only visualizes the metabolic regulatory mechanism of TNBC under single/dual-target inhibition of SLC7A11/GLUT1 and accurately evaluates its in vivo efficacy, but also achieves precise localization of the TNBC tumor invasion boundary. M1219 can be used for the research of triple-negative breast cancer[1].

In Vitro

M1219 (0-24 h) is a bifunctional near-infrared fluorescent probe that can specifically, stably and independently detect GSH and ATP in cell-free buffer systems. Its detection range matches physiological concentrations, with an EC50 value of 6.711 mM for GSH and 4.108 mM for ATP[1].
M1219 (20 μM; 15 min) enables specific and independent detection of endogenous GSH and ATP in live 4T1 cells, thus allowing visualization of the dynamic changes of these molecules under the action of redox and energy metabolism regulators[1].
M1219 (15-60 min) enables high-contrast fluorescence imaging in breast cancer specimens for precise identification of tumor boundaries, thereby accurately distinguishing cancerous tissues from adjacent tissues[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

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In Vivo

M1219 (0.5 mmol in 200 μL; 3 administrations; intravenous injection) enables real-time dual-channel near-infrared visualization of dynamic changes in glutathione (GSH) and adenosine triphosphate (ATP) in an orthotopic triple-negative breast cancer (TNBC) mouse model, and the fluorescence signal intensity shows a significant negative correlation with therapeutic efficacy[1].
M1219 (Intratumoral injection) enables precise near-infrared fluorescence-guided resection of subcutaneous triple-negative breast cancer (TNBC) tumors in mice, achieving a negative surgical margin of <0.1 mm through high-contrast differentiation between tumor and normal tissues[1].
M1219 (1 mmol in 100 μL; intravenous injection; observation for 24 h after administration) exhibits low in vivo toxicity and favorable biosafety in healthy BALB/c mice[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: BALB/c (female)[1]
Dosage: 0.5 mmol in 200 μL
Administration: tail vein injection; on days 0, 10, and 18 post-treatment initiation
Result: Showed similar dual-channel (GSH at 815 nm, ATP at 650 nm) fluorescence intensities in tumor regions across all treatment groups on day 0.
Reached GSH channel fluorescence intensity 0.091-fold of the PBS control group, and ATP channel fluorescence intensity 0.090-fold of the control group in tumors by day 18.
Confirmed ex vivo GSH and ATP channel fluorescence intensities of 0.1067-fold and 0.093-fold of the control group, respectively.
Demonstrated a significant negative correlation between fluorescence signal intensity and tumor growth inhibition and therapeutic efficacy.
Molecular Weight

1256.04

Formula

C76H83ClN8O5S
SMILES

CCN(C1=CC2=C(C3(C4=C(C5=C(CC4)C=C(C=C5)N(C)C)O2)C6=C(C(N3CCN7CCN(C(CC[N+]8=C(C(C)(C9=C8C=CC=C9)C)/C=C/C%10=C(/C(CCC%10)=C/C=C%11N(C%12=C(C/%11(C)C)C=CC=C%12)C)SC%13=CC=C(C=C%13)[N+]([O-])=O)=O)CC7)=O)C=CC=C6)C=C1)CC.[Cl-]
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Room temperature in continental US; may vary elsewhere.
Storage

Please store the product under the recommended conditions in the Certificate of Analysis.
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Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

M1219M 1219M-1219Fluorescent Dyeoxidative stressATPTNBCmouse modelsGSH4T1 cellstriple-negative breast cancerbreast cancermitochondriaInhibitorinhibitorinhibit

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