Bicinchoninic acid
Based on 8 publication(s) in Google Scholar
Bicinchoninic acid is a chelating agent targeting CuI. Bicinchoninic acid forms a water-soluble complex with CuI at a 2:1 stoichiometric ratio, which not only inhibits its oxidation and disproportionation reactions, but also mediates the CuI-catalyzed azide-alkyne cycloaddition click chemistry reaction to achieve bioconjugation of fluorophores or biotin with functionalized proteins. Bicinchoninic acid chelates Cu+ from proteins that reduce Cu2+, forming a purple compound detectable by colorimetry, and thus is used to determine the total protein concentration in solution. Bicinchoninic acid is a potential small-molecule candidate for AHAS inhibitors.
For research use only. We do not sell to patients.
- Purity: 99.81%
- CAS No.: 1245-13-2
- Formula: C20H12N2O4
- Molecular Weight:344.33
-
Storage:
4°C, protect from light, stored under nitrogen
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
-
Biological Activity
-
Chemical Information
-
Solvent & Solubility
- Purity & Documentation
- References
-
Help & FAQs
Publications Citing Use of MedChemExpress (MCE) Bicinchoninic acid
More- Adv Funct Mater. 2024 Mar 14.
- Cardiovasc Diabetol. 2019 Feb 2;18(1):15. [Abstract]
- Mater Today Bio. 2025 Feb 20:31:101598. [Abstract]
- Nano Today. 2025 Jun.
- Nano Today. 2025 Jun.
- Biologics. 2024 May 23:18:129-142. [Abstract]
- J Cancer. 2021 Feb 22;12(8):2243-2257. [Abstract]
- J Oncol. 2022 Aug 11;2022:5824617. [Abstract]
Biological Activity
Bicinchoninic acid (BCA) acts as a Cu (I) chelating stabilizer in CuI-catalyzed azide-alkyne cycloaddition reactions[1].
Bicinchoninic acid (BCA) enables quantitative analysis of carbohydrates in three types of soil extracellular polymeric substance (EPS) extracts[2].
Bicinchoninic acid (Compound 8) is a potential small-molecule candidate inhibitor of AHAS identified via virtual screening. It exhibits only 1.4 % inhibitory effect on rapeseed root growth at a concentration of 100 μg/mL, and shows no inhibitory activity at 10 μg/mL[3].
Bicinchoninic acid (BCA) is a core reagent in commonly used BCA protein quantification methods. Under alkaline conditions, protein complexes with Cu2+ and is reduced to Cu+. Bicinchoninic acid then binds with Cu+ to form a purple-blue complex. The absorbance of this complex is directly proportional to the protein concentration[4].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
-
CAS No. 1245-13-2
-
Appearance Solid
-
Molecular Weight 344.33
-
Formula C20H12N2O4
-
Color Off-white to gray
-
SMILES
OC(C1=C(C=CC=C2)C2=NC(C3=NC(C=CC=C4)=C4C(C(O)=O)=C3)=C1)=O
-
Shipping
Room temperature in continental US; may vary elsewhere.
-
Storage
4°C, protect from light, stored under nitrogen
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen)
Publications (8)
-
Journal Impact Factor
-
Most Recent
-
-
Cardiovasc Diabetol
SGLT2 inhibition with empagliflozin attenuates myocardial oxidative stress and fibrosis in diabetic mice heart. [Abstract]2019 Feb 2;18(1):15. PMID: 30710997 -
Mater Today Bio
A metal-polyphenol network-based iron supplement with improved stability and reduced gastrointestinal toxicity for iron deficiency anemia therapy. [Abstract]2025 Feb 20:31:101598. PMID: 40070867 -
-
-
Biologics
LncRNA GAS5 Modulates the Progression of Glioma Through Repressing miR-135b-5p and Upregulating APC. [Abstract]2024 May 23:18:129-142. PMID: 38817552 -
J Cancer
MMP9 and IGFBP1 Regulate Tumor Immune and Drive Tumor Progression in Clear Cell Renal Cell Carcinoma. [Abstract]2021 Feb 22;12(8):2243-2257. PMID: 33758602 -
J Oncol
Metformin Exhibits an Attractive Antineoplastic Effect on Human Endometrial Cancer by Regulating the Hippo Signaling Pathway. [Abstract]2022 Aug 11;2022:5824617. PMID: 36226249
Solvent & Solubility
DMSO : 2 mg/mL (5.81 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Purity & Documentation
-
Data Sheet (278 KB)
-
SDS (396 KB)
- English - EN (396 KB)
- Français - FR (396 KB)
- Deutsch - DE (396 KB)
- Norwegian - NO (396 KB)
- Español - ES (396 KB)
- Swedish - SV (396 KB)
- Italian - IT (396 KB)
- Korean - KR (396 KB)
- Portuguese - PT (396 KB)
-
Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 2.9042 mL | 14.5210 mL | 29.0419 mL | 72.6048 mL |
| 5 mM | 0.5808 mL | 2.9042 mL | 5.8084 mL | 14.5210 mL |
Bicinchoninic acid Related Classifications
HY-W004599 Related Classifications
Powered by Bioz