1245-13-2
Chemical Structure
Bicinchoninic acid
- CAS No.: 1245-13-2
- Formula:C20H12N2O4
- Molecular Weight:344.33
IUPAC Name: [2,2'-biquinoline]-4,4'-dicarboxylic acid
InChIKey: AFYNADDZULBEJA-UHFFFAOYSA-N
SMILES: OC(C1=C(C=CC=C2)C2=NC(C3=NC(C=CC=C4)=C4C(C(O)=O)=C3)=C1)=O
Biological Activity: Bicinchoninic acid is a chelating agent targeting CuI. Bicinchoninic acid forms a water-soluble complex with CuI at a 2:1 stoichiometric ratio, which not only inhibits its oxidation and disproportionation reactions, but also mediates the CuI-catalyzed azide-alkyne cycloaddition click chemistry reaction to achieve bioconjugation of fluorophores or biotin with functionalized proteins. Bicinchoninic acid chelates Cu+ from proteins that reduce Cu2+, forming a purple compound detectable by colorimetry, and thus is used to determine the total protein concentration in solution. Bicinchoninic acid is a potential small-molecule candidate for AHAS inhibitors[1][2][3][4].
| Cat. No. | Product Name | Purity | Description | Pricing | |||||||||||||||||||
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Bicinchoninic acid | 99.81% | Bicinchoninic acid is a chelating agent targeting CuI. Bicinchoninic acid forms a water-soluble complex with CuI at a 2:1 stoichiometric ratio, which not only inhibits its oxidation and disproportionation reactions, but also mediates the CuI-catalyzed azide-alkyne cycloaddition click chemistry reaction to achieve bioconjugation of fluorophores or biotin with functionalized proteins. Bicinchoninic acid chelates Cu+ from proteins that reduce Cu2+, forming a purple compound detectable by colorimetry, and thus is used to determine the total protein concentration in solution. Bicinchoninic acid is a potential small-molecule candidate for AHAS inhibitors. | ||||||||||||||||||||
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References
- [1]. Christen EH, et al. Evaluation of bicinchoninic acid as a ligand for copper(I)-catalyzed azide-alkyne bioconjugations. Organic & biomolecular chemistry. 2012 Sep 07;10(33):6629-32.
- [2]. Bublitz T A, et al. Optimization of the bicinchoninic acid assay for quantifying carbohydrates of soil extracellular polymeric substances. Plant and Soil, 2024, 498: 699‑709.
- [3]. Wang JG, et al. Identification of some novel AHAS inhibitors via molecular docking and virtual screening approach. Bioorg Med Chem. 2007 Jan 1;15(1):374-80. [Content Brief]
- [4]. Noble J E, et al. Chapter 8 Quantitation of Protein. In: Guide to Protein Purification, 2nd Edition. Methods in Enzymology, 2009, 463:73‑95.
