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BCA  (Synonyms: Disodium bicinchoninate)

Cat. No.: HY-15908 Purity: 99.76%
COA Handling Instructions

BCA (Disodium bicinchoninate) is the disodium salt of 2,2'-Biquinoline-4,4'-dicarboxylic acid, which can be used for the analysis and determination of copper and protein.

For research use only. We do not sell to patients.

BCA Chemical Structure

BCA Chemical Structure

CAS No. : 979-88-4

Size Price Stock Quantity
Free Sample (0.1 - 0.5 mg)   Apply Now  
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
ready for reconstitution
USD 28 In-stock
Solution
10 mM * 1 mL in DMSO USD 28 In-stock
Solid
500 mg USD 25 In-stock
1 g USD 40 In-stock
5 g USD 130 In-stock
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50 g   Get quote  

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This product is a controlled substance and not for sale in your territory.

Customer Review

Based on 4 publication(s) in Google Scholar

Top Publications Citing Use of Products
  • Biological Activity

  • Purity & Documentation

  • References

  • Customer Review

Description

BCA (Disodium bicinchoninate) is the disodium salt of 2,2'-Biquinoline-4,4'-dicarboxylic acid, which can be used for the analysis and determination of copper and protein[1][2][3].

Molecular Weight

388.28

Formula

C20H10N2Na2O4

CAS No.
Appearance

Solid

Color

White to light yellow

SMILES

O=C(O[Na])C1=CC(C2=NC3=C(C=CC=C3)C(C(O[Na])=O)=C2)=NC4=C1C=CC=C4

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, protect from light, stored under nitrogen

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen)

Solvent & Solubility
In Vitro: 

H2O : 100 mg/mL (257.55 mM; Need ultrasonic)

DMSO : 2 mg/mL (5.15 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.5755 mL 12.8773 mL 25.7546 mL
5 mM 0.5151 mL 2.5755 mL 5.1509 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo:

For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  PBS

    Solubility: 25 mg/mL (64.39 mM); Clear solution; Need ultrasonic

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).
Purity & Documentation

Purity: 99.76%

References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO / H2O 1 mM 2.5755 mL 12.8773 mL 25.7546 mL 64.3865 mL
5 mM 0.5151 mL 2.5755 mL 5.1509 mL 12.8773 mL
H2O 10 mM 0.2575 mL 1.2877 mL 2.5755 mL 6.4387 mL
15 mM 0.1717 mL 0.8585 mL 1.7170 mL 4.2924 mL
20 mM 0.1288 mL 0.6439 mL 1.2877 mL 3.2193 mL
25 mM 0.1030 mL 0.5151 mL 1.0302 mL 2.5755 mL
30 mM 0.0858 mL 0.4292 mL 0.8585 mL 2.1462 mL
40 mM 0.0644 mL 0.3219 mL 0.6439 mL 1.6097 mL
50 mM 0.0515 mL 0.2575 mL 0.5151 mL 1.2877 mL
60 mM 0.0429 mL 0.2146 mL 0.4292 mL 1.0731 mL
80 mM 0.0322 mL 0.1610 mL 0.3219 mL 0.8048 mL
100 mM 0.0258 mL 0.1288 mL 0.2575 mL 0.6439 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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BCA
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HY-15908
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