nNOS-IN-2 trihydrochloride
nNOS-IN-2 trihydrochloride is a blood-brain barrier-permeable nitric oxide synthase (nNOS) inhibitor. nNOS-IN-2 trihydrochloride exhibits Ki values of 1.7 nM and 2.3 nM against human and rat nNOS, respectively, and shows high selectivity for human eNOS and iNOS. nNOS-IN-2 trihydrochloride also effectively reduces PD-L1 expression in human melanoma cells under both basal conditions and IFN-γ exposure. nNOS-IN-2 trihydrochloride can be used for the study of melanoma and related signaling pathways.
For research use only. We do not sell to patients.
- CAS No.: 3124168-27-7
- Formula: C27H31Cl3N4O
- Molecular Weight:533.92
-
Storage:
Please store the product under the recommended conditions in the Certificate of Analysis.
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
-
Biological Activity
-
Chemical Information
- Purity & Documentation
- References
-
Help & FAQs
Biological Activity
|
nNOS 1.7 nM (Ki, human nNOS) |
nNOS 2.3 nM (Ki, rat nNOS) |
nNOS-IN-2 (compound 9) trihydrochloride induces cytotoxicity in human melanoma A375 and SK-MEL-28 cells, with EC50 values of 2.94 μM and 2.08 μM, respectively, and exhibits lower toxicity toward human primary fibroblasts than the lead compound HH044[1].
nNOS-IN-2 trihydrochloride (5 μM) reduces the basal PD-L1 expression level in human melanoma A375 cells to 70% of that in the control group, and effectively inhibits IFN-γ-induced PD-L1 upregulation at the concentration of 5 μM[1].
nNOS-IN-2 trihydrochloride reduces glutamate-induced NO accumulation in HT22 cells at a concentration of 0.5 μM[1].
nNOS-IN-2 trihydrochloride (200 μM; 17 h) exhibits moderate blood-brain barrier permeability, with an effective permeability of 6.91×10-6 cm/s at a concentration of 200 μM[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
-
Cell Line:human melanom A375
-
Concentration:5 μM
-
Incubation Time:with 250 μg/mL IFN-γ
-
Result:Reduced basal PD-L1 expression levels in human melanoma A375 cells to 70% of the control group.
Effectively inhibited IFN-γ-induced PD-L1 upregulation at a concentration of 5 μM.
Chemical Information
-
CAS No. 3124168-27-7
-
Molecular Weight 533.92
-
Formula C27H31Cl3N4O
-
SMILES
N=C(C1=CC=CO1)NC2=CC=CC(CNCCC3=CC=C(C4=CC=CC=C4)C=C3CN)=C2.Cl.Cl.Cl
-
Shipping
Room temperature in continental US; may vary elsewhere.
-
Storage
Please store the product under the recommended conditions in the Certificate of Analysis.
Purity & Documentation
References
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
nNOS-IN-2 trihydrochloride Related Classifications
HY-181545A Related Classifications
Powered by Bioz