2. Apoptosis
  3. Apoptosis
  4. Pitstop 2

Pitstop 2 is a clathrin inhibitor which inhibits clathrin-mediated endocytosis (CME) by associating with the terminal domain of clathrin. Pitstop 2 has the potential for anti-cancer research.

For research use only. We do not sell to patients.

CAS No. : 1419320-73-2

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Customer Review

Based on 30 publication(s) in Google Scholar

Pitstop 2 Related Antibodies

Top Publications Citing Use of Products

30 Publications Citing Use of MCE Pitstop 2

Flow Cytometry
RT-PCR
Cell Imaging/Staining
IF
WB

    Pitstop 2 purchased from MedChemExpress. Usage Cited in: Nat Immunol. 2025 Oct;26(10):1673-1685.  [Abstract]

    Representative immunofluorescence images, quantification of rPFO colocalization with the TGN and TGN dispersion in HeLa cells stably expressing scFv-sfGFP pre-treated with pitstop 2 (20 µM) for 1 h before addition of 0.90 nM 10xGCN4-rPFO. Scale bar, 5 μm.

    Pitstop 2 purchased from MedChemExpress. Usage Cited in: Nat Immunol. 2025 Oct;26(10):1673-1685.  [Abstract]

    Immunoblots of HEK293T cells stably expressing NLRP3, ASC, and caspase-1 (Casp1) pre-treated with 20 µM pitstop 2 for 1 h before addition of 0.90 nM rPFO for 40 min.

    Pitstop 2 purchased from MedChemExpress. Usage Cited in: Adv Funct Mater. 2025 Aug 3.

    Treatment with CytoD (1 μM, 6 h), MβCD (100 μM, 6 h), amiloride (50 μM, 6 h), or Pitstop 2 (50 μM, 6 h) significantly attenuated the intracellular green fluorescence of FITC/M-BSA@PSiNPs (A-B) and FITC/M-BSA (1C) in RAW 264.7 macrophages.

    Pitstop 2 purchased from MedChemExpress. Usage Cited in: Proc Natl Acad Sci U S A. 2025 Mar 25;122(12):e2502064122.  [Abstract]

    WNV entry into MITD1-expressing cells, assessed by RT-qPCR; PitStop2 at 50 µM for 2 h was used as control.

    Pitstop 2 purchased from MedChemExpress. Usage Cited in: Commun Biol. 2024 Dec 31;7(1):1719.  [Abstract]

    BT-474 and SKBR3 cells were assayed for Tmab internalization in presence of Pitstop2 (30 μM and 15 μM; 0-50 min).

    Pitstop 2 purchased from MedChemExpress. Usage Cited in: Nat Commun. 2023 Mar 15;14(1):1437.  [Abstract]

    Relative cellular uptake of Rhodamine-labeled nanogels with different stiffness after inhibited by Pitstop 2 (1 μM, 5 μM, 10 μM, 20 μM; 0.5 h) at different dosage in RAW 264.7 cells.

    • Biological Activity

    • Purity & Documentation

    • References

    • Customer Review

    Description

    Pitstop 2 is a clathrin inhibitor which inhibits clathrin-mediated endocytosis (CME) by associating with the terminal domain of clathrin. Pitstop 2 has the potential for anti-cancer research[1][2].

    In Vitro

    Pitstop 2 (20-40 μM; 30 min) inhibits the endocytosis of transferrin in J774A.1 macrophages and does not compromise cell viability[1].
    Pitstop 2 (20-40 μM; 30 min) does not affect the internalization of cholera toxin B in J774A.1 macrophages[1].
    Pitstop 2 (0.001-100 μM; 6 h) inhibits the mitotic spindle and impairs mitotic progression in HeLa cells[2].
    Pitstop 2 (1-30 μM; 24 h) induces apoptosis and inhibits cell growth in dividing cancer cells[2].
    Pitstop 2 (1-30 μM; 48 h) does not affect the growth and viability of non-tumourigenic NIH3T3 fibroblasts[2].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    Pitstop 2 Related Antibodies

    Cell Viability Assay[2]

    Cell Line: HeLa cells
    Concentration: 1, 3, 10, 30 μM
    Incubation Time: 24 hours
    Result: Reduced the total number of viable HeLa cells markedly in a dose-dependent manner.
    Molecular Weight

    473.36

    Formula

    C20H13BrN2O3S2
    CAS No.
    Appearance

    Solid

    Color

    Yellow to brown

    SMILES

    O=S(C1=C2C=CC=CC2=CC=C1)(NC(S/C3=C\C4=CC=C(Br)C=C4)=NC3=O)=O
    Shipping

    Room temperature in continental US; may vary elsewhere.
    Storage

    4°C, protect from light

    *In solvent : -80°C, 2 years; -20°C, 1 year (protect from light)

    Solvent & Solubility
    In Vitro: 

    DMSO : 50 mg/mL (105.63 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 2.1126 mL 10.5628 mL 21.1256 mL
    5 mM 0.4225 mL 2.1126 mL 4.2251 mL
    View the Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year (protect from light). When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

    • Molarity Calculator

    • Dilution Calculator

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

    Mass
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    Volume
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    Molecular Weight *

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    This equation is commonly abbreviated as: C1V1 = C2V2

    Concentration (start)

    C1

    ×
    Volume (start)

    V1

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    C2

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    V2

    In Vivo:

    Select the appropriate dissolution method based on your experimental animal and administration route.

    For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
    To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
    The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

    • Protocol 1

      Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

      Solubility: ≥ 2.5 mg/mL (5.28 mM); Clear solution

      This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

      Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

      Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
    In Vivo Dissolution Calculator
    Please enter the basic information of animal experiments:

    Dosage

    mg/kg

    Animal weight
    (per animal)

    g

    Dosing volume
    (per animal)

    μL

    Number of animals

    Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
    Please enter your animal formula composition:
    %
    DMSO +
    % 
    +
    %
    Tween-80 +
    %
    Saline
    Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
    The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
    Calculation results:
    Working solution concentration: mg/mL
    Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).

    *In solvent : -80°C, 2 years; -20°C, 1 year (protect from light)

    The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
    Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
     If the continuous dosing period exceeds half a month, please choose this protocol carefully.
    Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
    Purity & Documentation

    Purity: 98.42%

    SDS (394 KB)

    COA (259 KB) LCMS (98 KB)

    Handling Instructions (2659 KB)
    References

    Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year (protect from light). When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

    Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
    DMSO 1 mM 2.1126 mL 10.5628 mL 21.1256 mL 52.8139 mL
    5 mM 0.4225 mL 2.1126 mL 4.2251 mL 10.5628 mL
    10 mM 0.2113 mL 1.0563 mL 2.1126 mL 5.2814 mL
    15 mM 0.1408 mL 0.7042 mL 1.4084 mL 3.5209 mL
    20 mM 0.1056 mL 0.5281 mL 1.0563 mL 2.6407 mL
    25 mM 0.0845 mL 0.4225 mL 0.8450 mL 2.1126 mL
    30 mM 0.0704 mL 0.3521 mL 0.7042 mL 1.7605 mL
    40 mM 0.0528 mL 0.2641 mL 0.5281 mL 1.3203 mL
    50 mM 0.0423 mL 0.2113 mL 0.4225 mL 1.0563 mL
    60 mM 0.0352 mL 0.1760 mL 0.3521 mL 0.8802 mL
    80 mM 0.0264 mL 0.1320 mL 0.2641 mL 0.6602 mL
    100 mM 0.0211 mL 0.1056 mL 0.2113 mL 0.5281 mL
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    Pitstop 2 Related Classifications

    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    Pitstop 21419320-73-2Pitstop2Pitstop-2Apoptosisclathrinclathrin-mediatedendocytosisCMEcancerInhibitorinhibitorinhibit

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