Art. -Nr.: HY-115604

Pitstop 2

35 Cited Publications

Kundenbewertung

Based on 35 publication(s) in Google Scholar

Pitstop 2 is a clathrin inhibitor which inhibits clathrin-mediated endocytosis (CME) by associating with the terminal domain of clathrin. Pitstop 2 has the potential for anti-cancer research.

Nur für Forschungszwecke. Wir verkaufen nicht an Patienten.

Reinheit: 98.42%
CAS. Nr.: 1419320-73-2
Formel: C₂₀H₁₃BrN₂O₃S₂
Molecular Weight:473.36

Speicherung:

4°C, protect from light

* In solvent : -80°C, 2 years; -20°C, 1 year (protect from light)

Pitstop 2 Chemical Structure

CAS. Nr.: 1419320-73-2

Größe

Preis

Verfügbarkeit

Menge

Amount: USD 0.00

* Bitte wählen Sie Menge, bevor Sie Artikel hinzufügen.

Top Publications Citing

Use of MCE Products

Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biologische Aktivität
- Beschreibung
- In Vitro
Chemical Information
Publications (35)
Lösungsmittel & Löslichkeit
- In Vitro
- In Vivo
Reinheit & Dokumentation
Verweise
Help & FAQs
Classifications
Bioz

Biologische Aktivität

Beschreibung

Pitstop 2 is a clathrin inhibitor which inhibits clathrin-mediated endocytosis (CME) by associating with the terminal domain of clathrin. Pitstop 2 has the potential for anti-cancer research^[1]^[2].

In Vitro

Pitstop 2 (20-40 μM; 30 min) inhibits the endocytosis of transferrin in J774A.1 macrophages and does not compromise cell viability^[1].
Pitstop 2 (20-40 μM; 30 min) does not affect the internalization of cholera toxin B in J774A.1 macrophages^[1].
Pitstop 2 (0.001-100 μM; 6 h) inhibits the mitotic spindle and impairs mitotic progression in HeLa cells^[2].
Pitstop 2 (1-30 μM; 24 h) induces apoptosis and inhibits cell growth in dividing cancer cells^[2].
Pitstop 2 (1-30 μM; 48 h) does not affect the growth and viability of non-tumourigenic NIH3T3 fibroblasts^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Viability Assay^[2]

Cell Line:

HeLa cells
Concentration:

1, 3, 10, 30 μM
Incubation Time:

24 hours
Result:

Reduced the total number of viable HeLa cells markedly in a dose-dependent manner.

Chemical Information

CAS. Nr. 1419320-73-2
Appearance Solid
Molecular Weight 473.36
Formel C₂₀H₁₃BrN₂O₃S₂
Color Yellow to brown
SMILES

O=S(C1=C2C=CC=CC2=CC=C1)(NC(S/C3=C\C4=CC=C(Br)C=C4)=NC3=O)=O
Versand

Room temperature in continental US; may vary elsewhere.
Speicherung

4°C, protect from light

* In solvent : -80°C, 2 years; -20°C, 1 year (protect from light)

Publications (35)

All Flow CytometryRT-PCRCell Imaging/StainingIFWB

Sort by:

Journal Impact Factor

Cell

Efficient amyloid-β degradation in Alzheimer's disease using SPYTACs. [Abstract]

2026 Apr 2;189(7):1923-1941.e26. PMID: 41785850
Mol Cancer

Tumour-infiltrating microplastics disrupt the JAK-STAT-microbiota axis to promote immunotherapy resistance in colorectal cancer. [Abstract]

2026 Feb 10;25(1):73. PMID: 41664174
Immunity

Oxidized DNA fragments exit mitochondria via mPTP- and VDAC-dependent channels to activate NLRP3 inflammasome and interferon signaling. [Abstract]

2022 Aug 9;55(8):1370-1385.e8. PMID: 35835107
Adv Mater

Conformationally Variable Peptides Trap and Detoxify Ox-LDL in Plaques for Attenuating Atherosclerosis in Multiple Species. [Abstract]

2026 Jun;38(33):e21108. PMID: 42116761
Nat Immunol

Type A cholesterol-dependent cytolysins translocate to the trans-Golgi network for NLRP3 inflammasome activation. [Abstract]

2025 Oct;26(10):1673-1685. PMID: 40913097

Pitstop 2 purchased from MedChemExpress. Usage Cited in: Nat Immunol. 2025 Oct;26(10):1673-1685. [Abstract]

Representative immunofluorescence images, quantification of rPFO colocalization with the TGN and TGN dispersion in HeLa cells stably expressing scFv-sfGFP pre-treated with pitstop 2 (20 µM) for 1 h before addition of 0.90 nM 10xGCN4-rPFO. Scale bar, 5 μm.

Pitstop 2 purchased from MedChemExpress. Usage Cited in: Nat Immunol. 2025 Oct;26(10):1673-1685. [Abstract]

Immunoblots of HEK293T cells stably expressing NLRP3, ASC, and caspase-1 (Casp1) pre-treated with 20 µM pitstop 2 for 1 h before addition of 0.90 nM rPFO for 40 min.
Bone Res

Pak4-mediated crosstalk between necroptotic macrophages and tendon stem/progenitor cells contributes to traumatic heterotopic ossification formation. [Abstract]

2025 Oct 20;13(1):88. PMID: 41111094
Adv Funct Mater

2025 Aug 3.

Pitstop 2 purchased from MedChemExpress. Usage Cited in: Adv Funct Mater. 2025 Aug 3.

Typical confocal images of RAW 264.7 cells incubated with 100 μg/mL FITC/M-BSA@PSiNPs for 6 h, in the absence or presence of 50 μmol/L Pitstop 2, 100 μmol/L Methyl-β-cyclodextrin (MβCD), 50 μmol/L amiloride, or 1 μmol/L CytoD (scale bar = 40 μm).
Adv Funct Mater

2024 May 1.
Nat Commun

Clustering of NLRP3 induced by membrane or protein scaffolds promotes inflammasome assembly. [Abstract]

2025 May 27;16(1):4887. PMID: 40425567
Nat Commun

Mechano-boosting nanomedicine antitumour efficacy by blocking the reticuloendothelial system with stiff nanogels. [Abstract]

2023 Mar 15;14(1):1437. PMID: 36918575

Pitstop 2 purchased from MedChemExpress. Usage Cited in: Nat Commun. 2023 Mar 15;14(1):1437. [Abstract]

Relative cellular uptake of Rhodamine-labeled nanogels with different stiffness after inhibited by Pitstop 2 (1 μM, 5 μM, 10 μM, 20 μM; 0.5 h) at different dosage in RAW 264.7 cells.
ACS Nano

Uncovering the Importance of Ligand Mobility on Cellular Uptake of Nanoparticles: Insights from Experimental, Computational, and Theoretical Investigations. [Abstract]

2024 Feb 27;18(8):6463-6476. PMID: 38346263
Nat Chem Biol

Adhesion GPCR-induced ectocytosis mediates intercellular GPCR signal propagation. [Abstract]

2026 Feb 13. PMID: 41688711
J Nanobiotechnology

Size control of lipid nanoparticles via simulation-based design of a microfluidic chip and its effect on mRNA delivery in vitro and in vivo. [Abstract]

2025 Nov 23;23(1):797. PMID: 41276828
Adv Sci (Weinh)

Plac1+ Tumor Cell-Treg Interplay Supports Tumorigenesis and Progression of Head and Neck Cancer. [Abstract]

2025 May;12(17):e2417312. PMID: 40056047
Carbohydr Polym

Innate immune receptors co-recognition of polysaccharides initiates multi-pathway synergistic immune response. [Abstract]

2023 Apr 1:305:120533. PMID: 36737186
J Pharm Anal

Effect of cholesterol on distribution, cell uptake, and protein corona of lipid microspheres at sites of cardiovascular inflammatory injury. [Abstract]

2025 Jul;15(7):101182. PMID: 40735031
Proc Natl Acad Sci U S A

MITD1 is a brain-specific interferon-inducible factor that inhibits flavivirus replication. [Abstract]

2025 Mar 25;122(12):e2502064122. PMID: 40112111

Pitstop 2 purchased from MedChemExpress. Usage Cited in: Proc Natl Acad Sci U S A. 2025 Mar 25;122(12):e2502064122. [Abstract]

WNV entry into MITD1-expressing cells, assessed by RT-qPCR; PitStop2 at 50 µM for 2 h was used as control.
Int J Biol Macromol

Growth inhibition of pancreatic cancer by targeted delivery of gemcitabine via fucoidan-coated pH-sensitive liposomes. [Abstract]

2024 Aug 5;277(Pt 3):134517. PMID: 39111497
Cell Mol Gastroenterol Hepatol

OGT-enriched Hepatocyte-derived Extracellular Vesicles Promote Capillarization of Liver Sinusoidal Endothelial Cells in Metabolic Dysfunction-associated Steatotic Liver Disease. [Abstract]

2026 Jan 7;20(5):101721. PMID: 41513003
Environ Health

2025 Nov 6.
Small Sci

2025 Dec 15.
Bioeng Transl Med

Nanoalbumin-prodrug conjugates prepared via a thiolation-and-conjugation method improve cancer chemotherapy and immune checkpoint blockade therapy by promoting CD8+ T-cell infiltration. [Abstract]

2022 Jul 30;8(1):e10377. PMID: 36684090
Commun Biol

MMP1 and PRSS23 induce PAR2 biased agonism in painful oral cancers. [Abstract]

2026 May 11. PMID: 42115777
Commun Biol

Proteolysis targeting chimera (PROTAC)-driven antibody internalization of oncogenic cell surface receptors. [Abstract]

2024 Dec 31;7(1):1719. PMID: 39741170

Pitstop 2 purchased from MedChemExpress. Usage Cited in: Commun Biol. 2024 Dec 31;7(1):1719. [Abstract]

BT-474 and SKBR3 cells were assayed for Tmab internalization in presence of Pitstop2 (30 μM and 15 μM; 0-50 min).
Biol Res

DYRK1-mediated phosphorylation of endocytic components is required for extracellular lumen expansion in ascidian notochord. [Abstract]

2023 Mar 11;56(1):10. PMID: 36899423
Aquaculture

2026 May 4;623:744110.
Antiviral Res

Salinomycin inhibits orthopoxvirus infection in vitro and in vivo by blocking endosomal acidification. [Abstract]

2026 May:249:106403. PMID: 41941992
J Biol Chem

G6PD facilitates axon regeneration via clathrin-mediated endocytosis. [Abstract]

2026 Apr;302(4):111345. PMID: 41791708
J Virol

SFTSV utilizes AXL/GAS6 for entry via PI3K-PLC-dependent macropinocytosis activated by AXL-kinase. [Abstract]

2025 Aug 25:e0022125. PMID: 40853130
J Cell Sci

The Scribble-SGEF-Dlg1 complex regulates E-cadherin and ZO-1 stability, turnover and transcription in epithelial cells. [Abstract]

2024 Oct 1;137(19):jcs262181. PMID: 39350674
Traffic

AP2S1 regulates APP degradation through late endosome-lysosome fusion in cells and APP/PS1 mice. [Abstract]

2023 Jan;24(1):20-33. PMID: 36412210
Icahn School of Medicine at Mount Sinai

2026.
bioRxiv

2026 Mar 12.
Biomed Pharmacother

Drug target proteome profiling identifies HES1-driven mitotic catastrophe in ovarian serous carcinoma. [Abstract]

2025 Dec:193:118716. PMID: 41202420
Research Square Preprint

2021 Oct.

Lösungsmittel & Löslichkeit

In Vitro:

DMSO : 50 mg/mL (105.63 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

2.1126 mL

10.5628 mL

21.1256 mL

5 mM

0.4225 mL

2.1126 mL

4.2251 mL

10 mM

0.2113 mL

1.0563 mL

2.1126 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year (protect from light). When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Molaritätsrechner

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Konzentration Konzentration

Volumen Volumen

Molecular Weight Molecular Weight

Verdünnungsrechner

Konzentration _{(Stammlösung)} × Volumen _{(Stammlösung)} = Konzentration _{(Ziellösung)} × Volumen _{(Ziellösung)}

Konzentration _{(Stammlösung)} Konzentration _{(Stammlösung)}

Volumen _{(Stammlösung)} Volumen _{(Stammlösung)}

Konzentration _{(Ziellösung)} Konzentration _{(Ziellösung)}

Volumen _{(Ziellösung)} Volumen _{(Ziellösung)}

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

Stock Solution Working Solution

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline

Solubility: ≥ 2.5 mg/mL (5.28 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage Dosage
mg/kg
Animal Weight (Per Animal) Animal Weight (Per Animal)
g
Dosing Volume (Per Animal) Dosing Volume (Per Animal)
μL
Number of Animals Number of Animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

%
DMSO +

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
%
+
+
%
Tween-80 + +
%
Saline +

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Working solution concentration: 0.22 mg/mL

Reinheit & Dokumentation

Select Batch:

Reinheit: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (281 KB)
SDS (394 KB)
- English - EN (394 KB)
- Français - FR (394 KB)
- Deutsch - DE (394 KB)
- Norwegian - NO (394 KB)
- Español - ES (394 KB)
- Swedish - SV (394 KB)
- Italian - IT (394 KB)
- Korean - KR (394 KB)
- Portuguese - PT (394 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

Verweise

[1]. Kumar GA, et, al. Leishmania donovani Internalizes into Host Cells via Caveolin-mediated Endocytosis. Sci Rep. 2019 Sep 2; 9(1): 12636. [Content Brief]

[2]. Smith CM, et, al. Inhibition of clathrin by pitstop 2 activates the spindle assembly checkpoint and induces cell death in dividing HeLa cancer cells. Mol Cancer. 2013 Jan 17; 12:4. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
DMSO	1 mM	2.1126 mL	10.5628 mL	21.1256 mL	52.8139 mL
	5 mM	0.4225 mL	2.1126 mL	4.2251 mL	10.5628 mL
	10 mM	0.2113 mL	1.0563 mL	2.1126 mL	5.2814 mL
	15 mM	0.1408 mL	0.7042 mL	1.4084 mL	3.5209 mL
	20 mM	0.1056 mL	0.5281 mL	1.0563 mL	2.6407 mL
	25 mM	0.0845 mL	0.4225 mL	0.8450 mL	2.1126 mL
	30 mM	0.0704 mL	0.3521 mL	0.7042 mL	1.7605 mL
	40 mM	0.0528 mL	0.2641 mL	0.5281 mL	1.3203 mL
	50 mM	0.0423 mL	0.2113 mL	0.4225 mL	1.0563 mL
	60 mM	0.0352 mL	0.1760 mL	0.3521 mL	0.8802 mL
	80 mM	0.0264 mL	0.1320 mL	0.2641 mL	0.6602 mL
	100 mM	0.0211 mL	0.1056 mL	0.2113 mL	0.5281 mL

Keywords

Pitstop 2

35 Cited Publications

100 mg Angebot einholen

Subscribe to the

MedChemExpress

Newsletter

Produktname:
Art. -Nr.:
Menge:
MCE Japan Authorized Agent:

Produktname:
CAS. Nr.:
Species:
Reinheit:
Menge:

Pitstop 2

Covalent Screening Library

Targeted Diversity Library

Cell Death Library

Extracellular Vesicles (EVs) Compound Library

Bioactive Compound Library Max

Covalent Screening Library Plus

Bioactive Compound Library

Non-Alcoholic Fatty Liver Disease (NAFLD) Compound Library

High-Throughput Bioactive Compound Library

Apoptosis Compound Library

Anti-Cancer Compound Library

Publications Citing Use of MedChemExpress (MCE) Pitstop 2

Biologische Aktivität

Chemical Information

Publications (35)

Lösungsmittel & Löslichkeit

Reinheit & Dokumentation

Verweise

Help & FAQs

Complete Stock Solution Preparation Table

Pitstop 2

Covalent Screening Library

Targeted Diversity Library

Cell Death Library

Extracellular Vesicles (EVs) Compound Library

Bioactive Compound Library Max

Covalent Screening Library Plus

Bioactive Compound Library

Non-Alcoholic Fatty Liver Disease (NAFLD) Compound Library

High-Throughput Bioactive Compound Library

Apoptosis Compound Library

Anti-Cancer Compound Library

Publications Citing Use of MedChemExpress (MCE) Pitstop 2

Biologische Aktivität

Chemical Information

Publications (35)

Lösungsmittel & Löslichkeit

Reinheit & Dokumentation

Verweise

Help & FAQs

Complete Stock Solution Preparation Table

Pitstop 2 Related Classifications

HY-115604 Related Classifications