2. Apoptosis
  3. Apoptosis
  4. Pitstop 2

Pitstop 2 

Cat. No.: HY-115604 Purity: 98.38%
COA Handling Instructions

Pitstop 2 is a clathrin inhibitor which inhibits clathrin-mediated endocytosis (CME) by associating with the terminal domain of clathrin. Pitstop 2 has the potential for anti-cancer research.

For research use only. We do not sell to patients.

Pitstop 2 Chemical Structure

Pitstop 2 Chemical Structure

CAS No. : 1419320-73-2

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Customer Review

Based on 7 publication(s) in Google Scholar

Other Forms of Pitstop 2:

Pitstop 2 Related Antibodies

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  • Biological Activity

  • Purity & Documentation

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Description

Pitstop 2 is a clathrin inhibitor which inhibits clathrin-mediated endocytosis (CME) by associating with the terminal domain of clathrin. Pitstop 2 has the potential for anti-cancer research[1][2].

In Vitro

Pitstop 2 (20-40 μM; 30 min) inhibits the endocytosis of transferrin in J774A.1 macrophages and does not compromise cell viability[1].
Pitstop 2 (20-40 μM; 30 min) does not affect the internalization of cholera toxin B in J774A.1 macrophages[1].
Pitstop 2 (0.001-100 μM; 6 h) inhibits the mitotic spindle and impairs mitotic progression in HeLa cells[2].
Pitstop 2 (1-30 μM; 24 h) induces apoptosis and inhibits cell growth in dividing cancer cells[2].
Pitstop 2 (1-30 μM; 48 h) does not affect the growth and viability of non-tumourigenic NIH3T3 fibroblasts[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Pitstop 2 Related Antibodies

Cell Viability Assay[2]

Cell Line: HeLa cells
Concentration: 1, 3, 10, 30 μM
Incubation Time: 24 hours
Result: Reduced the total number of viable HeLa cells markedly in a dose-dependent manner.
Molecular Weight

473.36

Appearance

Solid

Formula

C20H13BrN2O3S2
CAS No.
SMILES

O=S(C1=C2C=CC=CC2=CC=C1)(NC(S/C3=C\C4=CC=C(Br)C=C4)=NC3=O)=O.[(Z)]
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

4°C, protect from light

*In solvent : -80°C, 2 years; -20°C, 1 year (protect from light)

Solvent & Solubility
In Vitro: 

DMSO : 62.5 mg/mL (132.03 mM; Need ultrasonic)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.1126 mL 10.5628 mL 21.1256 mL
5 mM 0.4225 mL 2.1126 mL 4.2251 mL
10 mM 0.2113 mL 1.0563 mL 2.1126 mL
*Please refer to the solubility information to select the appropriate solvent.
In Vivo:
  • 1.

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

    Solubility: ≥ 2.5 mg/mL (5.28 mM); Clear solution

*All of the co-solvents are available by MedChemExpress (MCE).
Purity & Documentation
References
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Pitstop 2 Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • Molarity Calculator

  • Dilution Calculator

The molarity calculator equation

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass   Concentration   Volume   Molecular Weight *
= × ×

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

Keywords:

Pitstop 21419320-73-2Pitstop2Pitstop-2Apoptosisclathrinclathrin-mediatedendocytosisCMEcancerInhibitorinhibitorinhibit

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