(+)-Totarol
Based on 1 Customer Validation
(+)-Totarol is a diterpenoid membrane-disrupting agent and membrane structure regulator found in plants of the genus Podocarpus spp. (+)-Totarol inserts into phospholipid model membranes, disrupts phospholipid packing, and impairs the functional integrity of membranes. (+)-Totarol exhibits antibacterial activity against a variety of bacterial species and β-lactam-resistant strains, and its action is associated with changes in membrane physical properties. (+)-Totarol can be used in studies related to bacterial infections.
For research use only. We do not sell to patients.
- Purity: 98.46%
- CAS No.: 511-15-9
- Formula: C20H30O
- Molecular Weight:286.45
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Storage:
4°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
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Biological Activity
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Cell Line
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Type | Value | Description | References |
|---|---|---|---|---|
| CHO | IC50 |
170.46 μM
Compound: 3
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Cytotoxicity against CHO cells after 48 hrs by MTT assay
Cytotoxicity against CHO cells after 48 hrs by MTT assay
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[PMID: 22196513] |
| MDCK | CC50 |
21 μM
Compound: 24
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Cytotoxicity against MDCK cells assessed as reduction of cell viability
Cytotoxicity against MDCK cells assessed as reduction of cell viability
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[PMID: 25815159] |
| MDCK | EC50 |
3.5 μM
Compound: 24
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Antiviral activity against oseltamivir/amantadine-resistant Influenza A virus (A/Puerto Rico/8/1934(H1N1)) infected in MDCK cells assessed as protection against virus-induced cytocidal effect after 48 hrs by CellTiter-Glo assay
Antiviral activity against oseltamivir/amantadine-resistant Influenza A virus (A/Puerto Rico/8/1934(H1N1)) infected in MDCK cells assessed as protection against virus-induced cytocidal effect after 48 hrs by CellTiter-Glo assay
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[PMID: 25815159] |
(+)-Totarol (0.5-35 mol%) dose-dependently modulates the phase transitions of DEPE vesicles, eliminating the Lα→HII transition at concentrations above 2 mol% and inducing complex low-temperature phase behavior between 5-35 mol%[2].
(+)-Totarol (5-30 mol%) decreases DMPC bilayer structural order below the main phase transition temperature and increases order above the transition temperature, completely abolishing the transition-associated anisotropy change at 30 mol%[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 511-15-9
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Appearance Solid
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Molecular Weight 286.45
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Formula C20H30O
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Color White to off-white
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SMILES
OC1=CC=C2[C@@]3(C)CCCC(C)(C)[C@]3([H])CCC2=C1C(C)C
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Structure Classification
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Initial Source
Apis mellifera
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
4°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
Solvent & Solubility
DMSO : 200 mg/mL (698.20 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL. * In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
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Data Sheet (274 KB)
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SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Portuguese - PT (393 KB)
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Handling Instructions (2659 KB)
References
[1]. Bernabeu A, et al. A MAS-NMR study of the location of (+)-totarol, a diterpenoid bioactive molecule, in phospholipid model membranes. Chem Phys Lipids. 2002;119(1-2):33-39. [Content Brief]
[2]. Micol V, et al. Effects of (+)-totarol, a diterpenoid antibacterial agent, on phospholipid model membranes. Biochim Biophys Acta. 2001 Apr 2;1511(2):281-90. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 3.4910 mL | 17.4551 mL | 34.9101 mL | 87.2753 mL |
| 5 mM | 0.6982 mL | 3.4910 mL | 6.9820 mL | 17.4551 mL | |
| 10 mM | 0.3491 mL | 1.7455 mL | 3.4910 mL | 8.7275 mL | |
| 15 mM | 0.2327 mL | 1.1637 mL | 2.3273 mL | 5.8184 mL | |
| 20 mM | 0.1746 mL | 0.8728 mL | 1.7455 mL | 4.3638 mL | |
| 25 mM | 0.1396 mL | 0.6982 mL | 1.3964 mL | 3.4910 mL | |
| 30 mM | 0.1164 mL | 0.5818 mL | 1.1637 mL | 2.9092 mL | |
| 40 mM | 0.0873 mL | 0.4364 mL | 0.8728 mL | 2.1819 mL | |
| 50 mM | 0.0698 mL | 0.3491 mL | 0.6982 mL | 1.7455 mL | |
| 60 mM | 0.0582 mL | 0.2909 mL | 0.5818 mL | 1.4546 mL | |
| 80 mM | 0.0436 mL | 0.2182 mL | 0.4364 mL | 1.0909 mL | |
| 100 mM | 0.0349 mL | 0.1746 mL | 0.3491 mL | 0.8728 mL |
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