Propylbenzilylcholine mustard 

Propylbenzilylcholine mustard (PrBCM) is an irreversible, covalently binding selective antagonist targeting rat cardiac muscarinic receptors, with a preference for muscarinic receptor subtypes that show low affinity for agonists. Propylbenzilylcholine mustard inactivates such receptors in a dose- and time-dependent manner in vitro, and its receptor selectivity remains stable across different ionic strengths and in GTP-containing buffer systems. Propylbenzilylcholine mustard can be applied to basic pharmacological studies on the structure and function of muscarinic receptors, especially focusing on the heterogeneity of cardiac muscarinic receptors and related cardiovascular pharmacological research^[1].

Propylbenzilylcholine mustard (3-10 nM; 0 to 40 min at 25 °C) inactivates rat heart muscarinic receptors with first-order kinetics at 3 nM, while at 10 nM, the inactivation rate slows over time, revealing reactivity with distinct receptor subclasses^[1].
Propylbenzilylcholine mustard (1-10 nM; 5 min at 25 °C) selectively inactivates low agonist affinity rat heart muscarinic receptors, leaving up to 90% of high agonist affinity [³H]-oxo-M-labeled receptors intact even after 50% inactivation of total [³H]-NMS-labeled receptors^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

375.89

C₂₁H₂₆ClNO₃

36167-80-3

O=C(C(C1=CC=CC=C1)(C2=CC=CC=C2)O)OCCN(CCC)CCCl

Room temperature in continental US; may vary elsewhere.

Please store the product under the recommended conditions in the Certificate of Analysis.

• [1]. Christophe J, et al. Propylbenzilylcholine mustard is selective for rat heart muscarinic receptors having a low affinity for agonists. Br J Pharmacol. 1986;88(1):63-70.  [Content Brief]