(Rac)-ZLc-002
Based on 1 Customer Validation
(Rac)-ZLc-002, an inhibitor of nNOS interaction with nitric oxide synthase 1 adaptor protein (NOS1AP), suppresses inflammatory nociception and chemotherapy-induced neuropathic pain and synergizes with Paclitaxel (HY-B0015) to reduce tumor cell viability.
For research use only. We do not sell to patients.
- Purity: 98.96%
- Formula: C10H17NO5
- Molecular Weight:231.25
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Storage:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
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Biological Activity
nNOS/NOS1AP interaction[1]
(Rac)-ZLc-002 (10 μM) reduces co-immunoprecipitation of NOS1AP with nNOS immunoprecipitated from primary cultured cortical neurons. (Rac)-ZLc-002 fails to disrupt nNOS–NOS1AP protein–protein interactions in the AlphaScreen in vitro binding assay. It shows no activity in a cell-free assay[1].
(Rac)-ZLc-002 (10 μM) reduces co-immunoprecipitation of full-length NOS1AP but not of PSD95-PDZ2 from HEK293T cells co-expressing full-length nNOS[1].
(Rac)-ZLc-002 (0-50 μM; 72 h) synergizes with Paclitaxel (HY-B0015) to reduce tumor cell viability[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:4T1 cells
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Concentration:0-50 µM
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Incubation Time:72 h
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Result:Treatment alone had no effect on the viability. Showed synergistic effect with Paclitaxel (HY-B0015).
(Rac)-ZLc-002 (4 and 10 mg/kg; i.p.; once) reduces formalin-evoked nociceptive behavior and Fos-like immunoreactivity in the spinal dorsal horn in rats[1].
(Rac)-ZLc-002 (40 mg/kg/day; i.v.; 7 days) attenuates chronic mild stress (CMS)‐induced anxiogenic behavior in ICR mice[2].
(Rac)-ZLc-002 (10 μM; 1 μl; 30 min after Corticosterone, HY-B1618) delivering into the hippocampus for 7 days reverses the behavioural effects of glucocorticoids in mice[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:Adult C57BL/6J male mice, paclitaxel model of neuropathic pain[1]
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Dosage:10 mg/kg
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Administration:IP, once or daily for 8 days
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Result:Increased postinjection mechanical paw withdrawal thresholds, mechanical paw withdrawal thresholds differed across postinjection times, and the interaction between drug treatment and injection time was significant. Elevated mechanical paw withdrawal thresholds relative to vehicle treatment from 30 min. Decreased postinjection cold responsiveness, cold responsiveness did not differ reliably across postinjection times, and the interaction between drug treatment and injection time was not significant. Once daily dosing increased mechanical paw withdrawal thresholds relative to the vehicle-treated group across the observation interval.
Chemical Information
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Appearance Solid
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Molecular Weight 231.25
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Formula C10H17NO5
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Color Off-white to light yellow
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SMILES
O=C(OC)CC(NC(C(OC)=O)C(C)C)=O
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Solvent & Solubility
DMSO : 100 mg/mL (432.43 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Purity & Documentation
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Data Sheet (274 KB)
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SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
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Handling Instructions (2659 KB)
References
[1]. Lee WH, Carey LM, Li LL, et al. ZLc002, a putative small-molecule inhibitor of nNOS interaction with NOS1AP, suppresses inflammatory nociception and chemotherapy-induced neuropathic pain and synergizes with paclitaxel to reduce tumor cell viability. Mol Pain. 2018;14:1744806918801224. [Content Brief]
[2]. Zhu LJ, Shi HJ, Chang L, et al. nNOS-CAPON blockers produce anxiolytic effects by promoting synaptogenesis in chronic stress-induced animal models of anxiety. Br J Pharmacol. 2020;177(16):3674-3690. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 4.3243 mL | 21.6216 mL | 43.2432 mL | 108.1081 mL |
| 5 mM | 0.8649 mL | 4.3243 mL | 8.6486 mL | 21.6216 mL | |
| 10 mM | 0.4324 mL | 2.1622 mL | 4.3243 mL | 10.8108 mL | |
| 15 mM | 0.2883 mL | 1.4414 mL | 2.8829 mL | 7.2072 mL | |
| 20 mM | 0.2162 mL | 1.0811 mL | 2.1622 mL | 5.4054 mL | |
| 25 mM | 0.1730 mL | 0.8649 mL | 1.7297 mL | 4.3243 mL | |
| 30 mM | 0.1441 mL | 0.7207 mL | 1.4414 mL | 3.6036 mL | |
| 40 mM | 0.1081 mL | 0.5405 mL | 1.0811 mL | 2.7027 mL | |
| 50 mM | 0.0865 mL | 0.4324 mL | 0.8649 mL | 2.1622 mL | |
| 60 mM | 0.0721 mL | 0.3604 mL | 0.7207 mL | 1.8018 mL | |
| 80 mM | 0.0541 mL | 0.2703 mL | 0.5405 mL | 1.3514 mL | |
| 100 mM | 0.0432 mL | 0.2162 mL | 0.4324 mL | 1.0811 mL |
(Rac)-ZLc-002 Related Classifications
HY-124996 Related Classifications
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