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THBS1 Protein, Mouse (His)

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Based on 2 publication(s) in Google Scholar

The THBS1 protein is a member of the thrombospondin family and plays a key role in cell-matrix interactions, angiogenesis, and tissue remodeling.THBS1 is known for its multi-receptor binding, including integrins and CD36, regulating signaling pathways related to cell adhesion, migration and survival.THBS1 Protein, Mouse (His) is the recombinant mouse-derived THBS1 protein, expressed by E.coli , with N-6*His labeled tag.

For research use only. We do not sell to patients.
  • Species: Mouse
  • Source: E. coli
  • Storage:
    Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
  • Experimental Validation Results for THBS1 Protein, Mouse (His)
  • Experimental Validation Results for THBS1 Protein, Mouse (His)
  • THBS1 Protein, Mouse (His) Product Image
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To place orders, for customer services and technical support, please contact:

609-228-6898
[email protected] [email protected]
Find Your Local Distributor Your local distributor: Hycultec GmbHHycultec
  • Biological Activity
  • Technical Parameters
  • Product Properties
  • Documentation
  • Help & FAQs
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Biological Activity

Description

The THBS1 protein is a member of the thrombospondin family and plays a key role in cell-matrix interactions, angiogenesis, and tissue remodeling.THBS1 is known for its multi-receptor binding, including integrins and CD36, regulating signaling pathways related to cell adhesion, migration and survival.THBS1 Protein, Mouse (His) is the recombinant mouse-derived THBS1 protein, expressed by E.coli , with N-6*His labeled tag.

Background

The THBS1 protein belongs to the thrombospondin family, a group of extracellular matrix proteins involved in various biological processes. THBS1 plays a crucial role in cell-matrix interactions, tissue remodeling, and angiogenesis. It is known for its ability to bind to multiple receptors, including integrins and CD36, and modulate signaling pathways involved in cell adhesion, migration, and survival. THBS1 is also implicated in the regulation of inflammation and immune responses. However, it should be noted that the THBS1 protein lacks conserved residue(s) required for the propagation of feature annotation, which may impact its function and interactions. Further research is needed to fully understand the significance of this observation and its implications for THBS1's role in physiological and pathological processes.

Verified Bioactivity

Measured by its binding ability in a functional ELISA. Immobilized Mouse THBS1 at 2 μg/mL can bind Biotinylated Mouse Decorin (HY-P70021). The ED50 for this effect is <0.4 μg/mL.

Technical Parameters

  • Species Mouse
  • Source E. coli
  • Tag N-6*His
  • Accession

    Q80YQ1 (N19-K350)

  • Gene ID
    21825
  • Molecular Construction
    • N-term
    • 6*His
    • THBS1 (N19-K350)
      Accession # Q80YQ1
    • C-term
  • Protein Length

    Partial

  • Synonyms

    THBS1; THBS; Thrombospondin 1; Thrombospondin-1p180; TSP1; Thrombospondin-1; TSP; Glycoprotein G; THBS-1; Thrombospondin-P50; TSP-1; THBS1 Protein

  • AA Sequence

    NRIPESGGDNGVFDIFELIGGARRGPGRRLVKGQDLSSPAFRIENANLIPAVPDDKFQDLLDAVWADKGFIFLASLRQMKKTRGTLLAVERKDNTGQIFSVVSNGKAGTLDLSLSLPGKQQVVSVEEALLATGQWKSITLFVQEDRAQLYIDCDKMESAELDVPIQSIFTRDLASVARLRVAKGDVNDNFQGVLQNVRFVFGTTPEDILRNKGCSSSATNVLLTLDNNVVNGSSPAIRTNYIGHKTKDLQAICGLSCDELSSMVLELKGLRTIVTTLQDSIRKVTEENRELVSELKRPPLCFHNGVQYKNNEEWTVDSCTECHCQNSVTICK

  • Predicted Molecular Mass

    40.6 kDa

  • Molecular Weight

    Approximately 37 kDa, based on SDS-PAGE under reducing conditions.

  • Purity

    ≥ 90%, as determined by reducing SDS-PAGE.

Product Properties

Appearance

Lyophilized powder

Formulation

Lyophilized from a 0.22 μm filtered solution of 10 mM Tris-HCl, 1 mM EDTA, 6% Trehalose, pH 8.0.
Note: For SPR assay, please replace the buffer. Primary amine components (e.g., Tris, imidazole) can affect protein-coupled chips.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.

Storage & Stability

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Calculators

Reconstitution Calculator

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

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Dilution Calculator

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

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The Specific Activity Calculator Equation
  • Specific Activity (Unit/mg)
  • Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) Specific Activity (Unit/mg)
Unit/mg
= 106 ÷
Biological Activity (ED50) Biological Activity (ED50)
106 ÷
ng/mL
MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     ≥ 90%, as determined by reducing SDS-PAGE.

  • 1/16

    MedChemExpress Validation
     Measured by its binding ability in a functional ELISA. When Mouse THBS1 is coated at 2 μg/mL (100 μL/well) can bind Mouse Decorin. The ED50 for this effect is 0.3512 μg/mL.

  • THBS1 Protein, Mouse (His) Product Image
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  • Experimental Validation Results for THBS1 Protein, Mouse (His)
  • Experimental Validation Results for THBS1 Protein, Mouse (His)
  • THBS1 Protein, Mouse (His) Product Image