IL-6 Protein, Mouse

IL-6 Protein is a multifunctional cytokine secreted by both immune and non-immune cells, and belongs to the interleukin family. IL-6 Protein plays a key role in immune responses, inflammatory reactions and other processes. IL-6 Protein, Mouse is a recombinant IL-6 protein expressed by E. coli without a tag^[1][2][3].

IL-6 is a multifunctional cytokine secreted by immune cells (such as T cells, B cells, and macrophages) and non-immune cells (such as fibroblasts, endothelial cells, and hepatocytes). IL-6 transmits signals by binding to its receptor. As a cytokine rapidly and transiently produced in response to infection and tissue damage, IL-6 participates in host defense by stimulating acute-phase responses, hematopoiesis, and immune reactions. Its dysregulation can trigger chronic inflammation and autoimmune diseases. In the initial stage of inflammation, IL-6 is transported via blood to the liver to induce the synthesis of multiple acute-phase proteins. It also participates in regulating serum iron and zinc levels and platelet release. In immune responses, IL-6 promotes Th17 differentiation, inhibits Treg differentiation, and affects immune tolerance^[1][2][3].

IL-6 Protein (Mouse; 25 ng/mL; 24 h) can promote the production of inflammatory factors MCP-1, MMP-3, CCL5, CXCL12 and the expression of hypertrophic markers COL10A1 and RUNX2 in primary mouse chondrocytes. The mechanism involves the activation of the JAK2/STAT3 pathway^[3].

IL-6 Protein (Mouse; 0.3 μg/kg; intra-articular injection; single dose) can cause spinal hyperexcitability in rats^[3].

1.The ED₅₀ is <0.02 ng/mL as measured by murine 7TD1 cells, corresponding to a specific activity of >5 × 10⁷ units/mg.
2. Measured by its binding ability in a functional ELISA. When Recombinant Mouse IL-6 Protein is immobilized at 5 μg/mL (100 µL/well) can bind Biotinylated Recombinant Mouse IL-6 R alpha (HY-P72538). The ED₅₀ for this effect is 20-156 ng/mL.
3. Measured by its binding ability in a functional ELISA. Immobilized Mouse IL-6 at 5 μg/mL (100 μL/well) on the plate can bind Mouse IL-6R alpha (HY-P72538). The ED₅₀ for this effect is 20-156 ng/mL.

• 
  Immobilized Mouse IL-6, His Tag at 5 μg/mL (100 μL/well) can bind Biotinylated Mouse IL-6 R alpha that produces 50% of the optimal binding response is found to be approximately 117.5 ng/mL.

Mouse

E. coli

Tag Free

P08505 (F25-T211)

16193  [NCBI]

Full Length of Mature Protein

FPTSQVRRGDFTEDTTPNRPVYTTSQVGGLITHVLWEIVEMRKELCNGNSDCMNNDDALAENNLKLPEIQRNDGCYQTGYNQEICLLKISSGLLEYHSYLEYMKNNLKDNKKDKARVLQRDTETLIHIFNQEVKDLHKIVLPTPISNALLTDKLESQKEWLRTKTIQFILKSLEEFLKVTLRSTRQT

Approximately 22 kDa, based on SDS-PAGE under reducing conditions.

• 
  ≥ 95%, as determined by reducing SDS-PAGE.

Lyophilized powder

1.Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.
2.Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4, 8% trehalose.
3.Lyophilized from a 0.22 μm filtered solution of 50 mM Tris-HCl, 200 mM NaCl, 500 mM arginine, pH 8.0.
Note: For SPR assay, please replace the buffer. Primary amine components (e.g., Tris, imidazole) can affect protein-coupled chips.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.

• [1]. Frorath B, et al. Production of recombinant rat interleukin-6 in Escherichia coli using a novel highly efficient expression vector pGEX-3T. Biotechniques. 1992 Apr;12(4):558-63.  [Content Brief]

  [2]. Tanaka T, et al. IL-6 in inflammation, immunity, and disease. Cold Spring Harb Perspect Biol. 2014 Sep 4;6(10):a016295.  [Content Brief]

  [3]. Mima Z, et al. Blockade of JAK2 retards cartilage degeneration and IL-6-induced pain amplification in osteoarthritis. Int Immunopharmacol. 2022 Dec;113(Pt A):109340.  [Content Brief]