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IL-6 Protein, Rat (HEK293)

4 Cited Publications
Customer Review

Based on 4 publication(s) in Google Scholar

IL-6 Protein, Rat (HEK293) is a HEK293 cell derived multifunctional cytokine that can regulate various immune and inflammatory responses.

For research use only. We do not sell to patients.
  • Species: Rat
  • Source: HEK293
  • Storage:
    Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
  • Experimental Validation Results for IL-6 Protein, Rat (HEK293)
  • Experimental Validation Results for IL-6 Protein, Rat (HEK293)
  • Experimental Validation Results for IL-6 Protein, Rat (HEK293)
  • Experimental Validation Results for IL-6 Protein, Rat (HEK293)
  • Experimental Validation Results for IL-6 Protein, Rat (HEK293)
  • Experimental Validation Results for IL-6 Protein, Rat (HEK293)
  • IL-6 Protein, Rat (HEK293) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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To place orders, for customer services and technical support, please contact:

609-228-6898
[email protected] [email protected]
Find Your Local Distributor Your local distributor: Hycultec GmbHHycultec
  • Biological Activity
  • Technical Parameters
  • Product Properties
  • Documentation
  • References
  • Help & FAQs
Top Publications Citing

Use of MCE Products

Biological Activity

Description

IL-6 Protein, Rat (HEK293) is a HEK293 cell derived multifunctional cytokine that can regulate various immune and inflammatory responses.

Background

Interleukin-6 (IL-6) is one of the most important mediators of the acute phase reaction in liver[1]. Interleukin 6 (IL-6), promptly and transiently produced in response to infections and tissue injuries, contributes to host defense through the stimulation of acute phase responses, hematopoiesis, and immune reactions. IL-6 promotes specific differentiation of naïve CD4+ T cells, thus performing an important function in the linking of innate to acquired immune response. IL-6, in combination with transforming growth factor (TGF)-β, is indispensable for Th17 differentiation from naïve CD4+ T cells, but that IL-6 also inhibits TGF-β-induced Treg differentiation[2].

Verified Bioactivity

1.The ED50 is <3 pg/mL as measured by 7TD1 Cells.
2.Measured in a cell proliferation assay using TF-1 cells. The ED50 for this effect is 16.72-24.74 pg/mL, corresponding to a specific activity is 4.04×107-5.981×107 units/mg.

Technical Parameters

  • Species Rat
  • Source HEK293
  • Tag Tag Free
  • Accession

    P20607 (F25-T211)

  • Gene ID
    24498  [NCBI]
  • Molecular Construction
    • N-term
    • IL-6 (F25-T211)
      Accession # P20607
    • C-term
  • Protein Length

    Full Length of Mature Protein

  • Synonyms

    IL6; Hybridoma Growth Factor; Prev. IFNB2; IFN-Beta-2; IL-6; BSF-2; Interleukin-6; CDF; BSF2; Interleukin 6 (Interferon, Beta 2); HGF; B-Cell Differentiation Factor; HSF; Truncated Interleukin 6; B-Cell Stimulatory Factor 2; Interferon, Beta 2; CTL Differ

  • AA Sequence

    FPTSQVRRGDFTEDTTHNRPVYTTSQVGGLITYVLREILEMRKELCNGNSDCMNSDDALSENNLKLPEIQRNDGCFQTGYNQEICLLKICSGLLEFRFYLEFVKNNLQDNKKDKARVIQSNTETLVHIFKQEIKDSYKIVLPTPTSNALLMEKLESQKEWLRTKTIQLILKALEEFLKVTMRSTRQT

  • Molecular Weight

    Approximately 19-26 kDa, based on SDS-PAGE under reducing conditions.

  • Purity

    ≥ 95%, as determined by reducing SDS-PAGE.

Product Properties

Appearance

Lyophilized powder

Formulation

Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Storage & Stability

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

References

[1]. Frorath B, et al. Production of recombinant rat interleukin-6 in Escherichia coli using a novel highly efficient expression vector pGEX-3T. Send to [Content Brief]

[2]. Tanaka T, et al. IL-6 in inflammation, immunity, and disease. Cold Spring Harb Perspect Biol. 2014 Sep 4;6(10):a016295. [Content Brief]

Calculators

Reconstitution Calculator

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

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Dilution Calculator

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The Specific Activity Calculator Equation
  • Specific Activity (Unit/mg)
  • Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) Specific Activity (Unit/mg)
Unit/mg
= 106 ÷
Biological Activity (ED50) Biological Activity (ED50)
106 ÷
ng/mL
MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     ≥ 95%, as determined by reducing SDS-PAGE.

  • 1/16

    MedChemExpress Validation
     Measured in a cell proliferation assay using TF-1 cells. The ED50 for this effect is 16.72 pg/mL, corresponding to a specific activity is 5.981×107 units/mg.

  • 1/16

    IL-6 Protein, Rat (HEK293) purchased from MedChemExpress. Usage Cited in: Free Radic Biol Med. 2024 Aug 20:221:188-202. [Abstract]

    Customer Validation
     IL-6 and TPEN can evoke STAT3 phosphorylation. H9c2 cells were exposed to IL-6 (20 ng/ml) for 30 min.

  • 1/16

    IL-6 Protein, Rat (HEK293) purchased from MedChemExpress. Usage Cited in: Free Radic Biol Med. 2024 Aug 20:221:188-202. [Abstract]

    Customer Validation
     Confocal images of H9c2 cells co-labeled with Cy3-p-STAT3 and DAPI. Cells were exposed to TPEN (10 μM) for 4 h or treated with IL-6 (20 ng/ml) for 30 min.

  • 1/16

    IL-6 Protein, Rat (HEK293) purchased from MedChemExpress. Usage Cited in: Int Immunopharmacol. 2022 Dec;113(Pt A):109340. [Abstract]

    Customer Validation
     Spinal cord neuronal responses to innocuous and noxious stimuli applied to the rat knee joint before and after IL-6 (0.3 µg/kg) injection.

  • 1/16

    IL-6 Protein, Rat (HEK293) purchased from MedChemExpress. Usage Cited in: Redox Biol. 2021 Jul:43:101994. [Abstract]

    Customer Validation
     Representative Western blot showing protein O-GlcNAc modification in H9c2 cells treated with Rat IL-6 protein (HEK293).

  • IL-6 Protein, Rat (HEK293) Product Image
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  • Experimental Validation Results for IL-6 Protein, Rat (HEK293)
  • Experimental Validation Results for IL-6 Protein, Rat (HEK293)
  • Experimental Validation Results for IL-6 Protein, Rat (HEK293)
  • Experimental Validation Results for IL-6 Protein, Rat (HEK293)
  • Experimental Validation Results for IL-6 Protein, Rat (HEK293)
  • Experimental Validation Results for IL-6 Protein, Rat (HEK293)
  • IL-6 Protein, Rat (HEK293) Product Image