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TNF-alpha/TNFSF2 Protein, Mouse (156a.a)

37 Cited Publications
Customer Review

Based on 37 publication(s) in Google Scholar

Tumour Necrosis Factor alpha (TNF alpha) is a potent pro-inflammatory cytokine. TNF alpha binds to its receptors, mainly TNFR1 and TNFR2, and then transmits molecular signals for biological functions such as inflammation and cell death. TNF alpha stimulates NF-κB pathway via TNFR2 promotes cancer growth, invasion, and metastasis. Anti-TNF-α MAb significantly suppresses the tumor development in colitis-associated cancer (CAC) mouse. TNF alpha as a proneurogenic factor activates the SAPK/JNK Pathway and can facilitate neuronal replacement and brain repair in response to brain injury. TNF-alpha/TNFSF2 Protein, Mouse (156a.a) is a recombinant protein consisting of 156 amino acids (L80-L235) and is produced in E. coli.

For research use only. We do not sell to patients.
  • Species: Mouse
  • Source: E. coli
  • Storage:
    Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
  • Experimental Validation Results for TNF-alpha/TNFSF2 Protein, Mouse (156a.a)
  • Experimental Validation Results for TNF-alpha/TNFSF2 Protein, Mouse (156a.a)
  • Experimental Validation Results for TNF-alpha/TNFSF2 Protein, Mouse (156a.a)
  • Experimental Validation Results for TNF-alpha/TNFSF2 Protein, Mouse (156a.a)
  • Experimental Validation Results for TNF-alpha/TNFSF2 Protein, Mouse (156a.a)
  • Experimental Validation Results for TNF-alpha/TNFSF2 Protein, Mouse (156a.a)
  • Experimental Validation Results for TNF-alpha/TNFSF2 Protein, Mouse (156a.a)
  • TNF-alpha/TNFSF2 Protein, Mouse (156a.a) Product Image
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  • Biological Activity
  • Technical Parameters
  • Product Properties
  • Documentation
  • References
  • Help & FAQs
Top Publications Citing

Use of MCE Products

Biological Activity

Description

Tumour Necrosis Factor alpha (TNF alpha) is a potent pro-inflammatory cytokine[1]. TNF alpha binds to its receptors, mainly TNFR1 and TNFR2, and then transmits molecular signals for biological functions such as inflammation and cell death[2]. TNF alpha stimulates NF-κB pathway via TNFR2 promotes cancer growth, invasion, and metastasis. Anti-TNF-α MAb significantly suppresses the tumor development in colitis-associated cancer (CAC) mouse[3]. TNF alpha as a proneurogenic factor activates the SAPK/JNK Pathway and can facilitate neuronal replacement and brain repair in response to brain injury[4]. TNF-alpha/TNFSF2 Protein, Mouse (156a.a) is a recombinant protein consisting of 156 amino acids (L80-L235) and is produced in E. coli.

Background

TNF alpha is produced by various types of cells including macrophages, monocytes, neutrophils, T cells, and NK-cells[2].
The amino acid sequence of human TNF alpha protein has low homology between mouse, rat, bovine, cynomolgus TNF alpha protein. While, human TNF alpha shares 94.85% aa sequence identity with cynomolgus TNF alpha protein, mouse TNF alpha shares 94.47% aa sequence identity with rat TNF alpha protein.
TNF alpha exists in two forms; a type II transmembrane protein (tmTNF-α) and a mature soluble protein (sTNF-α). TNF-α binds to its receptors, mainly TNFR1 and TNFR2, and then transmits molecular signals for biological functions such as inflammation and cell death. Both sTNF-α and tmTNF-α activate TNFR1, and process a death domain (DD) that interacts with the TNFR1-associated death domain (TRADD) adaptor protein. The TNFR2 signaling pathway is mainly activated by tmTNF-α. TNFR1 signaling tends to be pro-inflammatory and apoptotic. TNFR2 results in NF-κB and MAPKs and AKT activation, TNFR2 activation is associated with homeostatic bioactivities such as tissue regeneration, cell proliferation, and cell survival, as well as host defense and inflammation[1].
TNF-alpha is critical for normal immune response, abnormal secretion TNF alpha activates synovial fibroblasts, keratinocytes, osteoclasts, induces rheumatoid arthritis, inflammatory bowel disease, psoriatic arthritis (PsA), and noninfectious uveitis (NIU)[3]. TNF alpha positively regulates endogenous TNF-α expression levels independently of Pgp efflux activity, induces IHF cells proliferation[4]. TNF alpha in tissues may promote cancer growth, invasion, and metastasis. Besides, TNF alpha stimulates NF-κB pathway via TNFR2 and anti-TNF-α MAb significantly suppresses the tumor development in colitis-associated cancer (CAC) mouse[5]. TNF alpha as a proneurogenic factor activates the SAPK/JNK pathway and can facilitate neuronal replacement and brain repair in response to brain injury[6].

In Vitro

TNF alpha (mouse) (0, 2.5, 5, 10, 20, 40 ng/ml; 5 min) stimulates NF-κB Pathway and upregulates the phosphorylation of p65 and IκBα in CT26 cells in a dose-dependent manner[5].
TNF alpha (mouse) (1, 10 ng/mL; 7 days) induces neuronal differentiation in SVZ cells via TNFR1 activation[6].
TNF alpha (mouse) (1, 10; 100 ng/mL; 48 h) induces cell proliferation at 1 ng/mL and at high concentrations (10 and 100 ng/ml) TNF-α induces cell apoptosis in SVZ cells[6].

Verified Bioactivity

The ED50 is <0.1 ng/mL as measured by murine L-929 cells, corresponding to a specific activity of >1.0 × 107 units/mg.

Technical Parameters

  • Species Mouse
  • Source E. coli
  • Tag Tag Free
  • Accession

    P06804 (L80-L235)

  • Gene ID
    21926  [NCBI]
  • Molecular Construction
    • N-term
    • TGF-α (L80-L235)
      Accession # P06804
    • C-term
  • Protein Length

    Full Length of TNF soluble form

  • Synonyms

    TNF; Tumor Necrosis Factor (TNF Superfamily, Member 2); Prev. TNFA; Tumor Necrosis Factor-Alpha; TNF-Alpha; Tumor Necrotic Factor Alpha; TNFSF2; TNF Superfamily, Member 2; Tumor Necrosis Factor Ligand Superfamily Member 2; TNF, Macrophage-Derived; TNF-A;

  • AA Sequence

    LRSSSQNSSDKPVAHVVANHQVEEQLEWLSQRANALLANGMDLKDNQLVVPADGLYLVYSQVLFKGQGCPDYVLLTHTVSRFAISYQEKVNLLSAVKSPCPKDTPEGAELKPWYEPIYLGGVFQLEKGDQLSAEVNLPKYLDFAESGQVYFGVIAL

  • Predicted Molecular Mass

    17.3 kDa

  • Molecular Weight

    Approximately 17 kDa, based on SDS-PAGE under reducing conditions.

  • Purity

    ≥ 95%, as determined by reducing SDS-PAGE.

Product Properties

Appearance

Lyophilized powder

Formulation

1.Lyophilized from a 0.22 μm filtered solution of PBS.
2.Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.
3.Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4, 8% trehalose.
Please refer to the lot-specific COA for specific buffer information.

Endotoxin Level

<0.2 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Storage & Stability

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

References

[1]. Horiuchi T, et al. Transmembrane TNF-alpha: structure, function and interaction with anti-TNF agents. Rheumatology (Oxford). 2010 Jul;49(7):1215-28. [Content Brief]

[2]. Zelová H, et al. TNF-α signalling and inflammation: interactions between old acquaintances. Inflamm Res. 2013 Jul;62(7):641-51. [Content Brief]

[3]. El-Tahan RR, et al. TNF-α gene polymorphisms and expression. Springerplus. 2016 Sep 7;5(1):1508. [Content Brief]

[4]. Jang DI, et al. The Role of Tumor Necrosis Factor Alpha (TNF-α) in Autoimmune Disease and Current TNF-α Inhibitors in Therapeutics. Int J Mol Sci. 2021 Mar 8;22(5):2719. [Content Brief]

[5]. Berguetti T, et al. TNF-α Modulates P-Glycoprotein Expression and Contributes to Cellular Proliferation via Extracellular Vesicles. Cells. 2019 May 24;8(5):500. [Content Brief]

[6]. Onizawa M, et al. Signaling pathway via TNF-alpha/NF-kappaB in intestinal epithelial cells may be directly involved in colitis-associated carcinogenesis. Am J Physiol Gastrointest Liver Physiol. 2009 Apr;296(4):G850-9. [Content Brief]

[7]. Bernardino L, et al. Tumor necrosis factor-alpha modulates survival, proliferation, and neuronal differentiation in neonatal subventricular zone cell cultures. Stem Cells. 2008 Sep;26(9):2361-71. [Content Brief]

[8]. Matsuno H, et al. The role of TNF-alpha in the pathogenesis of inflammation and joint destruction in rheumatoid arthritis (RA): a study using a human RA/SCID mouse chimera. Rheumatology (Oxford). 2002 Mar;41(3):329-37. [Content Brief]

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Calculators

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The Specific Activity Calculator Equation
  • Specific Activity (Unit/mg)
  • Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) Specific Activity (Unit/mg)
Unit/mg
= 106 ÷
Biological Activity (ED50) Biological Activity (ED50)
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ng/mL
MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     ≥ 95%, as determined by reducing SDS-PAGE.

  • 1/16

    MedChemExpress Validation
     Measured in a cytotoxicity assay using L‑929 mouse fibroblast cells in the presence of the metabolic inhibitor actinomycin D. The ED50 for this effect is 13.68 pg/mL, corresponding to a specific activity is 7.31×107 units/mg.

  • 1/16

    TNF-alpha/TNFSF2 Protein, Mouse (156a.a) purchased from MedChemExpress. Usage Cited in: J Nanobiotechnology. 2025 Feb 25;23(1):140. [Abstract]

    Customer Validation
     MSCs-EVs protein content under different concentrations of inflammatory factors (TNF-α-EV (0, 20, 50, 100, 200, and 500 ng/mL for 48 h)) pretreatment.

  • 1/16

    TNF-alpha/TNFSF2 Protein, Mouse (156a.a) purchased from MedChemExpress. Usage Cited in: Sci Adv. 2024 Nov 29;10(48):eadp9413. [Abstract]

    Customer Validation
     Changes in fire rates of SFO and OVLT neurons in response to 1nM IL-1β or TNF-α(a half hour in regular ACSF at 30°C)

  • 1/16

    TNF-alpha/TNFSF2 Protein, Mouse (156a.a) purchased from MedChemExpress. Usage Cited in: Proc Natl Acad Sci U S A. 2024 Apr 2;121(14):e2308132121. [Abstract]

    Customer Validation
     TNF: i.p., 50 µg/kg, once. Immunostaining of liver, kidney, and lung tissues and image quantification illustrating the effect of FKC (40 mg/kg) in reducing macrophage activation in both male and female mice as characterized through CD68-positive signals.

  • 1/16

    TNF-alpha/TNFSF2 Protein, Mouse (156a.a) purchased from MedChemExpress. Usage Cited in: Proc Natl Acad Sci U S A. 2024 Apr 2;121(14):e2308132121. [Abstract]

    Customer Validation
     TNF (i.p., 0-40 mg/kg, once) induces an increase in the plasma cytokines levels of TNF, MCP-1, IFN-γ, IL-1α, IL-1β, and IL-6 in both male and female mice, and FKC reduces all cytokine levels in a dose-dependent manner.

  • 1/16

    TNF-alpha/TNFSF2 Protein, Mouse (156a.a) purchased from MedChemExpress. Usage Cited in: Adv Healthc Mater. 2023 Jul;12(19):e2203051. [Abstract]

    Customer Validation
     Western blot images of p-p65, p65, IκBα, and MAP2 in NSCs co-cultured with 0 µg mL−1 Laponite nanoplatelets (Ctrl), 10 µg mL−1 Laponite nanoplatelets (LAP), 10 ng mL−1 TNF-α (TNF-α) (2 h), 10 µg mL−1 Laponite nanoplatelets, or 10 µm BAY11-7082 (2 h) (LAP+BAY) on day 5.

  • TNF-alpha/TNFSF2 Protein, Mouse (156a.a) Product Image
    1/16
  • Experimental Validation Results for TNF-alpha/TNFSF2 Protein, Mouse (156a.a)
  • Experimental Validation Results for TNF-alpha/TNFSF2 Protein, Mouse (156a.a)
  • Experimental Validation Results for TNF-alpha/TNFSF2 Protein, Mouse (156a.a)
  • Experimental Validation Results for TNF-alpha/TNFSF2 Protein, Mouse (156a.a)
  • Experimental Validation Results for TNF-alpha/TNFSF2 Protein, Mouse (156a.a)
  • Experimental Validation Results for TNF-alpha/TNFSF2 Protein, Mouse (156a.a)
  • Experimental Validation Results for TNF-alpha/TNFSF2 Protein, Mouse (156a.a)
  • TNF-alpha/TNFSF2 Protein, Mouse (156a.a) Product Image

  • TNF-alpha/TNFSF2 Protein, Mouse (156a.a) purchased from MedChemExpress. Usage Cited in: J Nanobiotechnology. 2025 Feb 25;23(1):140. [Abstract]

    MSCs-EVs protein content under different concentrations of inflammatory factors (TNF-α-EV (0, 20, 50, 100, 200, and 500 ng/mL for 48 h)) pretreatment.

  • TNF-alpha/TNFSF2 Protein, Mouse (156a.a) purchased from MedChemExpress. Usage Cited in: Sci Adv. 2024 Nov 29;10(48):eadp9413. [Abstract]

    Changes in fire rates of SFO and OVLT neurons in response to 1nM IL-1β or TNF-α(a half hour in regular ACSF at 30°C)

  • TNF-alpha/TNFSF2 Protein, Mouse (156a.a) purchased from MedChemExpress. Usage Cited in: Proc Natl Acad Sci U S A. 2024 Apr 2;121(14):e2308132121. [Abstract]

    TNF: i.p., 50 µg/kg, once. Immunostaining of liver, kidney, and lung tissues and image quantification illustrating the effect of FKC (40 mg/kg) in reducing macrophage activation in both male and female mice as characterized through CD68-positive signals.

  • TNF-alpha/TNFSF2 Protein, Mouse (156a.a) purchased from MedChemExpress. Usage Cited in: Proc Natl Acad Sci U S A. 2024 Apr 2;121(14):e2308132121. [Abstract]

    TNF (i.p., 0-40 mg/kg, once) induces an increase in the plasma cytokines levels of TNF, MCP-1, IFN-γ, IL-1α, IL-1β, and IL-6 in both male and female mice, and FKC reduces all cytokine levels in a dose-dependent manner.

  • TNF-alpha/TNFSF2 Protein, Mouse (156a.a) purchased from MedChemExpress. Usage Cited in: Adv Healthc Mater. 2023 Jul;12(19):e2203051. [Abstract]

    Western blot images of p-p65, p65, IκBα, and MAP2 in NSCs co-cultured with 0 µg mL−1 Laponite nanoplatelets (Ctrl), 10 µg mL−1 Laponite nanoplatelets (LAP), 10 ng mL−1 TNF-α (TNF-α) (2 h), 10 µg mL−1 Laponite nanoplatelets, or 10 µm BAY11-7082 (2 h) (LAP+BAY) on day 5.