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  2. Cytokines and Growth Factors
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  4. IL-2
  5. IL-2 Protein, Mouse

IL-2 Protein, Mouse

Cat. No.: HY-P7077
COA Handling Instructions

IL-2 Protein, Mouse is an immunomodulatory cytokine with widely varying functions including promotion of apoptosis, proliferation and survival of lymphocytes.

For research use only. We do not sell to patients.

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Top Publications Citing Use of Products
  • Biological Activity

  • Technical Parameters

  • Properties

  • Documentation

  • References

Description

IL-2 Protein, Mouse is an immunomodulatory cytokine with widely varying functions including promotion of apoptosis, proliferation and survival of lymphocytes.

Background

Interleukin-2 (IL-2) is a multi-faceted cytokine, known for promoting proliferation, survival, and cell death depending on the cell type and state. For example, IL-2 facilitates cell death only in activated T cells when antigen and IL-2 are abundant. The availability of IL-2 clearly impacts this process. IL-2 is retained in blood vessels by heparan sulfate, and that biologically active IL-2 is released from vessel tissue by heparanase. The morphologic changes and rapid cell death induced by dimeric IL-2 imply that cell death is mediated by disruption of membrane permeability and subsequent necrosis. IL-2 has a direct and unexpectedly broad influence on cellular homeostatic mechanisms in both immune and non-immune systems[1].

Biological Activity

Measured in a cell proliferation assay using CTLL-2 Cells. The ED50 this effect is ≤ 0.5117 ng/mL, corresponding to a specific activity is ≥ 1.954×106 units/mg.

  • Measured in a cell proliferation assay using CTLL-2 Cells. The ED50 for this effect is ≤ 0.5117 ng/mL, corresponding to a specific activity is ≥ 1.954×106 units/mg.
Species

Mouse

Source

E. coli

Tag

Tag Free

Accession

P04351 (A21-Q169)

Gene ID
Molecular Construction
N-term
IL-2 (A21-Q169)
Accession # P04351
C-term
Synonyms
rMuIL-2; IL2; T-cell Growth Factor; TCGF; Aldesleukin
AA Sequence

APTSSSTSSSTAEAQQQQQQQQQQQQHLEQLLMDLQELLSRMENYRNLKLPRMLTFKFYLPKQATELKDLQCLEDELGPLRHVLDLTQSKSFQLEDAENFISNIRVTVVKLKGSDNTFECQFDDESATVVDFLRRWIAFCQSIISTSPQ

Molecular Weight

Approximately 15-20 kDa

Purity
  • Greater than 95% as determined by reducing SDS-PAGE.
Appearance

Lyophilized powder

Formulation

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4 or 20 mM PB, 150 mM NaCl, pH 7.4 or 20 mM Sodium Citrate, 0.2% Tween 80, pH 3.0.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Storage & Stability

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Documentation
References

IL-2 Protein, Mouse Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • Reconstitution Calculator

  • Dilution Calculator

  • Specific Activity Calculator

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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IL-2 Protein, Mouse
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