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IL-2 Protein, Mouse

29 Cited Publications
Customer Review

Based on 29 publication(s) in Google Scholar

IL-2 protein is mainly produced by activated CD4-positive helper T cells and plays an important role in immune response and tolerance. Binding to IL-2R induces downstream signaling through JAK1 and JAK3 phosphorylation, thereby activating various pathways, including STAT, PI3K, and MAPK. IL-2 Protein, Mouse is the recombinant mouse-derived IL-2 protein, expressed by E. coli.

For research use only. We do not sell to patients.
  • Species: Mouse
  • Source: E. coli
  • Storage:
    Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
  • Experimental Validation Results for IL-2 Protein, Mouse
  • Experimental Validation Results for IL-2 Protein, Mouse
  • IL-2 Protein, Mouse Product Image
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  • Biological Activity
  • Technical Parameters
  • Product Properties
  • Documentation
  • References
  • Help & FAQs
Top Publications Citing

Use of MCE Products

Biological Activity

Description

IL-2 protein is mainly produced by activated CD4-positive helper T cells and plays an important role in immune response and tolerance. Binding to IL-2R induces downstream signaling through JAK1 and JAK3 phosphorylation, thereby activating various pathways, including STAT, PI3K, and MAPK. IL-2 Protein, Mouse is the recombinant mouse-derived IL-2 protein, expressed by E. coli.

Background

Interleukin-2 (IL-2) is a multi-faceted cytokine, known for promoting proliferation, survival, and cell death depending on the cell type and state. For example, IL-2 facilitates cell death only in activated T cells when antigen and IL-2 are abundant. The availability of IL-2 clearly impacts this process. IL-2 is retained in blood vessels by heparan sulfate, and that biologically active IL-2 is released from vessel tissue by heparanase. The morphologic changes and rapid cell death induced by dimeric IL-2 imply that cell death is mediated by disruption of membrane permeability and subsequent necrosis. IL-2 has a direct and unexpectedly broad influence on cellular homeostatic mechanisms in both immune and non-immune systems[1].

In Vivo

IL-2 Protein, Mouse (1 μg/kg, ip, once daily for 5 days) increases the frequency and function of regulatory T cells (Tregs), improves the survival rate of mice in transplantation model[2].
IL-2 Protein, Mouse (5 μg/kg, ip, once daily for 5 days) ameliorates the pulmonary edema and neutrophil aggregation, increases the level of anti-inflammatory cytokine IL-10 in mouse transfusion-related acute lung injury model[3].

Verified Bioactivity

1.Measured in a cell proliferation assay using CTLL-2 Cells. The ED50 for this effect is ≤0.5117 ng/mL, corresponding to a specific activity is ≥1.954×106 units/mg.
2.Measured in a cell proliferation assay using CTLL 2 mouse cytotoxic T cells.The specific activity of Recombinant Mouse IL-2 is ≥1×106 IU/mg.

Results
  • Experimental Validation Results for IL-2 Protein, Mouse
    Measured in a cell proliferation assay using CTLL-2 Cells. The ED50 for this effect is 0.5117 ng/mL, corresponding to a specific activity is 1.954×106 units/mg.

Technical Parameters

  • Species Mouse
  • Source E. coli
  • Tag Tag Free
  • Accession

    P04351 (A21-Q169)

  • Gene ID
    16183  [NCBI]
  • Molecular Construction
    • N-term
    • IL-2 (A21-Q169)
      Accession # P04351
    • C-term
  • Protein Length

    Full Length of Mature Protein

  • Synonyms

    rMuIL-2; IL2; T-cell Growth Factor; TCGF; Aldesleukin

  • AA Sequence

    APTSSSTSSSTAEAQQQQQQQQQQQQHLEQLLMDLQELLSRMENYRNLKLPRMLTFKFYLPKQATELKDLQCLEDELGPLRHVLDLTQSKSFQLEDAENFISNIRVTVVKLKGSDNTFECQFDDESATVVDFLRRWIAFCQSIISTSPQ

  • Predicted Molecular Mass

    17.4 kDa

  • Molecular Weight

    Approximately 17 kDa, based on SDS-PAGE under reducing conditions.

  • Purity

    ≥ 95%, as determined by reducing SDS-PAGE.

    • Experimental Validation Results for IL-2 Protein, Mouse
      ≥ 95%, as determined by reducing SDS-PAGE.

Product Properties

Appearance

Lyophilized powder

Formulation

1.Lyophilized from a 0.22 μm filtered solution of 20 mM sodium citrate, 0.02% Tween 80, pH 3.0.
2.Lyophilized from a 0.22 μm filtered solution of 20 mM sodium citrate, 0.02% Tween 80, 4% mannitol, pH 3.0.
3.Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.
4.Lyophilized from a 0.22 μm filtered solution of 20 mM sodium citrate, 0.2% Tween 80, pH 3.0.
5.Lyophilized from a 0.22 μm filtered solution of 20 mM PB, 150 mM NaCl, pH 7.4.
6.Lyophilized from a 0.22 μm filtered solution of 10 mM Sodium Citrate, pH 4.0.
Please refer to the lot-specific COA for specific buffer information.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Storage & Stability

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

References

[1]. Wrenshall LE, et al. Identification of a cytotoxic form of dimeric interleukin-2 in murine tissues. PLoS One. 2014 Jul 14;9(7):e102191. [Content Brief]

[2]. Tahvildari M, et al., Low-Dose IL-2 Therapy in Transplantation, Autoimmunity, and Inflammatory Diseases. J Immunol. 2019 Dec 1;203(11):2749-2755. [Content Brief]

[3]. He R, et al., Preventing murine transfusion-related acute lung injury by expansion of CD4+ CD25+ FoxP3+ Tregs using IL-2/anti-IL-2 complexes. Transfusion. 2019 Feb;59(2):534-544. [Content Brief]

Calculators

Reconstitution Calculator

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

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Dilution Calculator

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

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The Specific Activity Calculator Equation
  • Specific Activity (Unit/mg)
  • Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) Specific Activity (Unit/mg)
Unit/mg
= 106 ÷
Biological Activity (ED50) Biological Activity (ED50)
106 ÷
ng/mL
MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

  • ≥ 95%, as determined by reducing SDS-PAGE.

  • Measured in a cell proliferation assay using CTLL-2 Cells. The ED50 for this effect is 0.5117 ng/mL, corresponding to a specific activity is 1.954×106 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     ≥ 95%, as determined by reducing SDS-PAGE.

  • 1/16

    MedChemExpress Validation
     Measured in a cell proliferation assay using CTLL-2 Cells. The ED50 for this effect is 0.5117 ng/mL, corresponding to a specific activity is 1.954×106 units/mg.

  • IL-2 Protein, Mouse Product Image
    1/16
  • Experimental Validation Results for IL-2 Protein, Mouse
  • Experimental Validation Results for IL-2 Protein, Mouse
  • IL-2 Protein, Mouse Product Image