Copy Link Print Information

THBS1 Protein, Human (HEK293, His)

3 Cited Publications
Customer Review

Based on 3 publication(s) in Google Scholar

The THBS1 protein is an adhesion glycoprotein that controls multiple cellular processes and mediates cell-cell and cell-matrix interactions. It plays multifunctional roles in inflammation, angiogenesis, wound healing, ROS and NO signaling, apoptosis, senescence, senescence, self-renewal, stemness, and cardiovascular/metabolic homeostasis. THBS1 Protein, Human (HEK293, His) is the recombinant human-derived THBS1 protein, expressed by HEK293 , with C-10*His labeled tag.

For research use only. We do not sell to patients.
  • Species: Human
  • Source: HEK293
  • Storage:
    Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
  • Experimental Validation Results for THBS1 Protein, Human (HEK293, His)
  • Experimental Validation Results for THBS1 Protein, Human (HEK293, His)
  • Experimental Validation Results for THBS1 Protein, Human (HEK293, His)
  • Experimental Validation Results for THBS1 Protein, Human (HEK293, His)
  • Experimental Validation Results for THBS1 Protein, Human (HEK293, His)
  • THBS1 Protein, Human (HEK293, His) Product Image
Size
Price
Stock
Quantity
Amount: USD 0.00
Please select quantity
or
or

* Please select Quantity before adding items.

To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

Service
Data Download

To place orders, for customer services and technical support, please contact:

609-228-6898
[email protected] [email protected]
Find Your Local Distributor Your local distributor: Hycultec GmbHHycultec
  • Biological Activity
  • Technical Parameters
  • Product Properties
  • Documentation
  • Help & FAQs
Top Publications Citing

Use of MCE Products

Biological Activity

Description

The THBS1 protein is an adhesion glycoprotein that controls multiple cellular processes and mediates cell-cell and cell-matrix interactions. It plays multifunctional roles in inflammation, angiogenesis, wound healing, ROS and NO signaling, apoptosis, senescence, senescence, self-renewal, stemness, and cardiovascular/metabolic homeostasis. THBS1 Protein, Human (HEK293, His) is the recombinant human-derived THBS1 protein, expressed by HEK293 , with C-10*His labeled tag.

Background

THBS1 Protein, an adhesive glycoprotein, orchestrates a myriad of cellular processes through its mediation of both cell-to-cell and cell-to-matrix interactions. With a multifunctional role, it is intricately involved in inflammation, angiogenesis, wound healing, reactive oxygen species (ROS) and nitrous oxide (NO) signaling, apoptosis, senescence, aging, cellular self-renewal, stemness, and the maintenance of cardiovascular and metabolic homeostasis. This versatile protein negatively modulates dendritic cell activation and cytokine release, contributing to inflammation resolution and immune homeostasis. Acting as a ligand for receptors CD47 and CD36, THBS1 plays a pivotal role in modulating nitrous oxide (NO) signaling and blood pressure regulation. Additionally, it influences endothelial cell senescence by activating NADPH oxidase NOX1 and contributes to excess ROS production. THBS1 further inhibits stem cell self-renewal, regulates wound healing, induces apoptosis in podocytes, and suppresses angiogenesis through its interactions with CD47 and CD36. The protein's involvement in various cellular processes underscores its significance in physiological and pathological conditions, ranging from metabolic dysfunction in obesity to the ER stress response. As a homotrimer with disulfide linkages, THBS1 exhibits binding affinity for various extracellular matrix components and integrins, emphasizing its pivotal role in cellular interactions and signaling pathways.

Verified Bioactivity

1.Measured by its binding ability in a functional ELISA. Immobilized Human VEGF165 Protein (HY-P7110A) at 1 µg/mL (100 µL/well) can bind Biotinylated Human THBS1 Protein. The ED50 for this effect is <400 ng/mL.
2.Measured by its binding ability in a functional ELISA. Immobilized Human VEGF183 Protein at 1 µg/mL (100 µL/well) can bind Human THBS1 Protein. The ED50 for this effect is 275.0 ng/mL.

Technical Parameters

  • Species Human
  • Source HEK293
  • Tag C-10*His
  • Accession

    P07996-1 (N19-P1170)

  • Gene ID
    7057  [NCBI]
  • Molecular Construction
    • N-term
    • THBS1 (N19-P1170)
      Accession # P07996
    • 10*His
    • C-term
  • Protein Length

    Full Length of Mature Protein

  • Synonyms

    THBS1; THBS; Thrombospondin 1; Thrombospondin-1p180; TSP1; Thrombospondin-1; TSP; Glycoprotein G; THBS-1; Thrombospondin-P50; TSP-1; THBS1 Protein

  • AA Sequence

    NRIPESGGDNSVFDIFELTGAARKGSGRRLVKGPDPSSPAFRIEDANLIPPVPDDKFQDLVDAVRAEKGFLLLASLRQMKKTRGTLLALERKDHSGQVFSVVSNGKAGTLDLSLTVQGKQHVVSVEEALLATGQWKSITLFVQEDRAQLYIDCEKMENAELDVPIQSVFTRDLASIARLRIAKGGVNDNFQGVLQNVRFVFGTTPEDILRNKGCSSSTSVLLTLDNNVVNGSSPAIRTNYIGHKTKDLQAICGISCDELSSMVLELRGLRTIVTTLQDSIRKVTEENKELANELRRPPLCYHNGVQYRNNEEWTVDSCTECHCQNSVTICKKVSCPIMPCSNATVPDGECCPRCWPSDSADDGWSPWSEWTSCSTSCGNGIQQRGRSCDSLNNRCEGSSVQTRTCHIQECDKRFKQDGGWSHWSPWSSCSVTCGDGVITRIRLCNSPSPQMNGKPCEGEARETKACKKDACPINGGWGPWSPWDICSVTCGGGVQKRSRLCNNPTPQFGGKDCVGDVTENQICNKQDCPIDGCLSNPCFAGVKCTSYPDGSWKCGACPPGYSGNGIQCTDVDECKEVPDACFNHNGEHRCENTDPGYNCLPCPPRFTGSQPFGQGVEHATANKQVCKPRNPCTDGTHDCNKNAKCNYLGHYSDPMYRCECKPGYAGNGIICGEDTDLDGWPNENLVCVANATYHCKKDNCPNLPNSGQEDYDKDGIGDACDDDDDNDKIPDDRDNCPFHYNPAQYDYDRDDVGDRCDNCPYNHNPDQADTDNNGEGDACAADIDGDGILNERDNCQYVYNVDQRDTDMDGVGDQCDNCPLEHNPDQLDSDSDRIGDTCDNNQDIDEDGHQNNLDNCPYVPNANQADHDKDGKGDACDHDDDNDGIPDDKDNCRLVPNPDQKDSDGDGRGDACKDDFDHDSVPDIDDICPENVDISETDFRRFQMIPLDPKGTSQNDPNWVVRHQGKELVQTVNCDPGLAVGYDEFNAVDFSGTFFINTERDDDYAGFVFGYQSSSRFYVVMWKQVTQSYWDTNPTRAQGYSGLSVKVVNSTTGPGEHLRNALWHTGNTPGQVRTLWHDPRHIGWKDFTAYRWRLSHRPKTGFIRVVMYEGKKIMADSGPIYDKTYAGGRLGLFVFSQEMVFFSDLKYECRDP

  • Predicted Molecular Mass

    129 kDa

  • Molecular Weight

    Approximately 130 kDa & 170 kDa, based on SDS-PAGE under reducing conditions.

  • Glycosylation

    Yes

  • Purity

    ≥ 80%, as determined by reducing SDS-PAGE.

Product Properties

Appearance

Lyophilized powder

Formulation

1.Lyophilized from a 0.22 μm filtered solution of 20 mM PB, 8% trehalose, 4% mannitol, 200 mM NaCl, 0.02% Tween80, pH 6.5.
2.Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4, 5% trehalose, 5% mannitol and 0.01% Tween80.
3.Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4, 8% trehalose.
Please refer to the lot-specific COA for specific buffer information.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.

Storage & Stability

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Calculators

Reconstitution Calculator

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) Volume (to add to vial)
=
Mass (in vial) Mass (in vial)
÷
Desired Reconstitution Concentration Desired Reconstitution Concentration
Dilution Calculator

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

Concentration (start) Concentration (start)
×
Volume (start) Volume (start)
=
Concentration (final) Concentration (final)
×
Volume (final) Volume (final)
The Specific Activity Calculator Equation
  • Specific Activity (Unit/mg)
  • Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) Specific Activity (Unit/mg)
Unit/mg
= 106 ÷
Biological Activity (ED50) Biological Activity (ED50)
106 ÷
ng/mL
MOQ
Minimum order quantity
100 mg

Get Quote In-stock

Other size
Get Quote
Please select quantity
Amount: USD 0.00

Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     ≥ 80%, as determined by reducing SDS-PAGE.

  • 1/16

    MedChemExpress Validation
     Measured by its binding ability in a functional ELISA. Immobilized Human VEGF165 Protein (HY-P7110A) at 1 µg/mL (100µL/well) can bind Biotinylated Human THBS1 Protein. The ED50 for this effect is 361.5 ng/mL.

  • 1/16

    THBS1 Protein, Human (HEK293, His) purchased from MedChemExpress. Usage Cited in: Invest Ophthalmol Vis Sci. 2026 Jan 5;67(1):10. [Abstract]

    Customer Validation
     HTK cells were treated with THBS1 Protein, Human (0, 1, and 10 µg/mL) for 24 hours, and then the expression level of A2M was detected by quantitative PCR.

  • 1/16

    THBS1 Protein, Human (HEK293, His) purchased from MedChemExpress. Usage Cited in: Invest Ophthalmol Vis Sci. 2026 Jan 5;67(1):10. [Abstract]

    Customer Validation
     HTK cells were treated with THBS1 Protein, Human (0, 1, and 10 µg/mL) for 24 hours, and then the expression level of A2M was detected by Western blotting.

  • 1/16

    THBS1 Protein, Human (HEK293, His) purchased from MedChemExpress. Usage Cited in: Cell Discov. 2023 Mar 13;9(1):28. [Abstract]

    Customer Validation
     Representative images of EdU and Ki67 staining in OLK organoids after THBS1 Protein, Human (1 μg/mL) treatment.

  • THBS1 Protein, Human (HEK293, His) Product Image
    1/16
  • Experimental Validation Results for THBS1 Protein, Human (HEK293, His)
  • Experimental Validation Results for THBS1 Protein, Human (HEK293, His)
  • Experimental Validation Results for THBS1 Protein, Human (HEK293, His)
  • Experimental Validation Results for THBS1 Protein, Human (HEK293, His)
  • Experimental Validation Results for THBS1 Protein, Human (HEK293, His)
  • THBS1 Protein, Human (HEK293, His) Product Image

  • THBS1 Protein, Human (HEK293, His) purchased from MedChemExpress. Usage Cited in: Invest Ophthalmol Vis Sci. 2026 Jan 5;67(1):10. [Abstract]

    HTK cells were treated with THBS1 Protein, Human (0, 1, and 10 µg/mL) for 24 hours, and then the expression level of A2M was detected by quantitative PCR.

  • THBS1 Protein, Human (HEK293, His) purchased from MedChemExpress. Usage Cited in: Invest Ophthalmol Vis Sci. 2026 Jan 5;67(1):10. [Abstract]

    HTK cells were treated with THBS1 Protein, Human (0, 1, and 10 µg/mL) for 24 hours, and then the expression level of A2M was detected by Western blotting.

  • THBS1 Protein, Human (HEK293, His) purchased from MedChemExpress. Usage Cited in: Cell Discov. 2023 Mar 13;9(1):28. [Abstract]

    Representative images of EdU and Ki67 staining in OLK organoids after THBS1 Protein, Human (1 μg/mL) treatment.