THBS1 Protein, Human (HEK293, His)

The THBS1 protein is an adhesion glycoprotein that controls multiple cellular processes and mediates cell-cell and cell-matrix interactions. It plays multifunctional roles in inflammation, angiogenesis, wound healing, ROS and NO signaling, apoptosis, senescence, senescence, self-renewal, stemness, and cardiovascular/metabolic homeostasis. THBS1 Protein, Human (HEK293, His) is the recombinant human-derived THBS1 protein, expressed by HEK293 , with C-10*His labeled tag.

THBS1 Protein, an adhesive glycoprotein, orchestrates a myriad of cellular processes through its mediation of both cell-to-cell and cell-to-matrix interactions. With a multifunctional role, it is intricately involved in inflammation, angiogenesis, wound healing, reactive oxygen species (ROS) and nitrous oxide (NO) signaling, apoptosis, senescence, aging, cellular self-renewal, stemness, and the maintenance of cardiovascular and metabolic homeostasis. This versatile protein negatively modulates dendritic cell activation and cytokine release, contributing to inflammation resolution and immune homeostasis. Acting as a ligand for receptors CD47 and CD36, THBS1 plays a pivotal role in modulating nitrous oxide (NO) signaling and blood pressure regulation. Additionally, it influences endothelial cell senescence by activating NADPH oxidase NOX1 and contributes to excess ROS production. THBS1 further inhibits stem cell self-renewal, regulates wound healing, induces apoptosis in podocytes, and suppresses angiogenesis through its interactions with CD47 and CD36. The protein's involvement in various cellular processes underscores its significance in physiological and pathological conditions, ranging from metabolic dysfunction in obesity to the ER stress response. As a homotrimer with disulfide linkages, THBS1 exhibits binding affinity for various extracellular matrix components and integrins, emphasizing its pivotal role in cellular interactions and signaling pathways.

1.Measured by its binding ability in a functional ELISA. Immobilized Human VEGF165 Protein (HY-P7110A) at 1 µg/mL (100 µL/well) can bind Biotinylated Human THBS1 Protein. The ED₅₀ for this effect is <400 ng/mL.
2.Measured by its binding ability in a functional ELISA. Immobilized Human VEGF183 Protein at 1 µg/mL (100 µL/well) can bind Human THBS1 Protein. The ED₅₀ for this effect is 275.0 ng/mL.

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  Measured by its binding ability in a functional ELISA. Immobilized Human VEGF165 Protein (HY-P7110A) at 1 µg/mL (100µL/well) can bind Biotinylated Human THBS1 Protein. The ED₅₀ for this effect is 361.5 ng/mL.

Human

HEK293

C-10*His

P07996-1 (N19-P1170)

7057  [NCBI]

Full Length of Mature Protein

NRIPESGGDNSVFDIFELTGAARKGSGRRLVKGPDPSSPAFRIEDANLIPPVPDDKFQDLVDAVRAEKGFLLLASLRQMKKTRGTLLALERKDHSGQVFSVVSNGKAGTLDLSLTVQGKQHVVSVEEALLATGQWKSITLFVQEDRAQLYIDCEKMENAELDVPIQSVFTRDLASIARLRIAKGGVNDNFQGVLQNVRFVFGTTPEDILRNKGCSSSTSVLLTLDNNVVNGSSPAIRTNYIGHKTKDLQAICGISCDELSSMVLELRGLRTIVTTLQDSIRKVTEENKELANELRRPPLCYHNGVQYRNNEEWTVDSCTECHCQNSVTICKKVSCPIMPCSNATVPDGECCPRCWPSDSADDGWSPWSEWTSCSTSCGNGIQQRGRSCDSLNNRCEGSSVQTRTCHIQECDKRFKQDGGWSHWSPWSSCSVTCGDGVITRIRLCNSPSPQMNGKPCEGEARETKACKKDACPINGGWGPWSPWDICSVTCGGGVQKRSRLCNNPTPQFGGKDCVGDVTENQICNKQDCPIDGCLSNPCFAGVKCTSYPDGSWKCGACPPGYSGNGIQCTDVDECKEVPDACFNHNGEHRCENTDPGYNCLPCPPRFTGSQPFGQGVEHATANKQVCKPRNPCTDGTHDCNKNAKCNYLGHYSDPMYRCECKPGYAGNGIICGEDTDLDGWPNENLVCVANATYHCKKDNCPNLPNSGQEDYDKDGIGDACDDDDDNDKIPDDRDNCPFHYNPAQYDYDRDDVGDRCDNCPYNHNPDQADTDNNGEGDACAADIDGDGILNERDNCQYVYNVDQRDTDMDGVGDQCDNCPLEHNPDQLDSDSDRIGDTCDNNQDIDEDGHQNNLDNCPYVPNANQADHDKDGKGDACDHDDDNDGIPDDKDNCRLVPNPDQKDSDGDGRGDACKDDFDHDSVPDIDDICPENVDISETDFRRFQMIPLDPKGTSQNDPNWVVRHQGKELVQTVNCDPGLAVGYDEFNAVDFSGTFFINTERDDDYAGFVFGYQSSSRFYVVMWKQVTQSYWDTNPTRAQGYSGLSVKVVNSTTGPGEHLRNALWHTGNTPGQVRTLWHDPRHIGWKDFTAYRWRLSHRPKTGFIRVVMYEGKKIMADSGPIYDKTYAGGRLGLFVFSQEMVFFSDLKYECRDP

Approximately 130 kDa, based on SDS-PAGE under reducing conditions, due to the glycosylation.

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  ≥ 80%, as determined by reducing SDS-PAGE.

Lyophilized powder

1.Lyophilized from a 0.22 μm filtered solution of 20 mM PB, 8% trehalose, 4% mannitol, 200 mM NaCl, 0.02% Tween80, pH 6.5.
2.Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4, 5% trehalose, 5% mannitol and 0.01% Tween80.
3.Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4, 8% trehalose.
Please refer to the lot-specific COA for specific buffer information.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.