Copy Link Print Information

Serpin E1 Protein, Human (HEK293, His)

2 Cited Publications
Customer Review

Based on 2 publication(s) in Google Scholar

Serpin E1 is a serine protease inhibitor that regulates various cellular processes by inhibiting TMPRSS7, tissue-type plasminogen activator (PLAT), and urokinase-type plasminogen activator (PLAU). During fibrinolysis, it downregulates PLAT to control thrombus degradation and acts as a PLAU inhibitor to regulate cell adhesion. Serpin E1 Protein, Human (HEK293, His) is the recombinant human-derived Serpin E1 protein, expressed by HEK293 , with C-6*His labeled tag.

For research use only. We do not sell to patients.
  • Species: Human
  • Source: HEK293
  • Storage:
    Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
  • Experimental Validation Results for Serpin E1 Protein, Human (HEK293, His)
  • Serpin E1 Protein, Human (HEK293, His) Product Image
Size
Price
Stock
Quantity
Amount: USD 0.00
Please select quantity
or
or

* Please select Quantity before adding items.

To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

Service
Data Download

To place orders, for customer services and technical support, please contact:

609-228-6898
[email protected] [email protected]
Find Your Local Distributor Your local distributor: Hycultec GmbHHycultec
  • Biological Activity
  • Technical Parameters
  • Product Properties
  • Documentation
  • Help & FAQs
Top Publications Citing

Use of MCE Products

Biological Activity

Description

Serpin E1 is a serine protease inhibitor that regulates various cellular processes by inhibiting TMPRSS7, tissue-type plasminogen activator (PLAT), and urokinase-type plasminogen activator (PLAU). During fibrinolysis, it downregulates PLAT to control thrombus degradation and acts as a PLAU inhibitor to regulate cell adhesion. Serpin E1 Protein, Human (HEK293, His) is the recombinant human-derived Serpin E1 protein, expressed by HEK293 , with C-6*His labeled tag.

Background

Serpin E1, a serine protease inhibitor, exerts its regulatory influence on various cellular processes. It inhibits TMPRSS7 and serves as the primary inhibitor for both tissue-type plasminogen activator (PLAT) and urokinase-type plasminogen activator (PLAU). In the context of fibrinolysis, Serpin E1 is crucial for down-regulating PLAT, contributing to the controlled degradation of blood clots. Simultaneously, as a PLAU inhibitor, it plays a role in modulating cell adhesion and spreading. Beyond its function as a protease inhibitor, Serpin E1 acts as a regulator of cell migration, impacting processes such as keratinocyte migration during cutaneous injury repair. Its involvement extends to cellular and replicative senescence, contributing to alveolar type 2 cell senescence in the lung. Furthermore, Serpin E1 participates in the regulation of cementogenic differentiation in periodontal ligament stem cells and influences odontoblast differentiation and dentin formation during odontogenesis. The protein engages in various interactions, forming a heterodimer with TMPRSS7 and interacting with multiple partners, including VTN, PPP1CB, PLAUR/uPAR, SORL1, and LRP1, among others, highlighting its multifaceted role in diverse cellular pathways.

Verified Bioactivity

Measured by its ability to inhibit uPA cleavage of a peptide substrate, N-carbobenzyloxy-Gly-Gly-Arg-7-amido-4-methylcoumarin (Z-GGR-AMC). The IC50 value is <15 nM, as measured under the described conditions.

Assay Procedure

Materials
Assay buffer: 50 mM Tris, 0.01% (v/v) Tween? 20, pH 8.5;
Serpin E1 Protein, Human (HEK293, His) (HY-P71077)
PLAU/uPA Protein, Human (411a.a, HEK293, His, solution) (uPA, HY-P71050)
Substrate: Z-Gly-Gly-Arg-AMC (HY-P0019A)

Procedure
1. Dilute Human uPA to 2 μg/mL with assay buffer.
2. Prepare different concentrations of Serpin E1 with assay buffer by making the following serial dilutions: 1500, 750, 375, 187.5, 125, 75, 50, 25, and 12.5 nM.
3. Equal volumes of Human Serpin E/PAI-1 curves were mixed with diluted Human uPA at 2 μg/mL. Include 2 controls containing only Human uPA and assay buffer.
4. Mix the above mixture and incubate for 15 minutes at room temperature.
5. After incubation, the mixture was diluted 10-fold in assay buffer.
6. Dilute the substrate to 200 μM with assay buffer.
7. In a 96-well plate, add 50 μL of reaction mixture and start the reaction with 50 μL of 20 μM substrate.
8. Excitation and emission wavelengths 380 nm and 460 nm were read in kinetic mode for 5 minutes.
9. The 50% inhibitory concentration (IC50) of Human Serpin E1 was derived by plotting RFU/min versus concentration with a 4-PL fit.

Technical Parameters

  • Species Human
  • Source HEK293
  • Tag C-6*His
  • Accession

    P05121-1 (V24-P402)

  • Gene ID
    5054  [NCBI]
  • Molecular Construction
    • N-term
    • Serpin E1 (V24-P402)
      Accession # P05121-1
    • 6*His
    • C-term
  • Protein Length

    Full Length of Isoform-1 Mature Protein

  • Synonyms

    SERPINE1; Endothelial Plasminogen Activator Inhibitor; Prev. PLANH1; Serpin E1; Prev. PAI1; PAI-1; PAI; Serpin Peptidase Inhibitor, Clade E (Nexin, Plasminogen Activator Inhibitor Type 1), Member 1; Serine (Or Cysteine) Proteinase Inhibitor, Clade E (Nexi

  • AA Sequence

    VHHPPSYVAHLASDFGVRVFQQVAQASKDRNVVFSPYGVASVLAMLQLTTGGETQQQIQAAMGFKIDDKGMAPALRHLYKELMGPWNKDEISTTDAIFVQRDLKLVQGFMPHFFRLFRSTVKQVDFSEVERARFIINDWVKTHTKGMISNLLGKGAVDQLTRLVLVNALYFNGQWKTPFPDSSTHRRLFHKSDGSTVSVPMMAQTNKFNYTEFTTPDGHYYDILELPYHGDTLSMFIAAPYEKEVPLSALTNILSAQLISHWKGNMTRLPRLLVLPKFSLETEVDLRKPLENLGMTDMFRQFQADFTSLSDQEPLHVAQALQKVKIEVNESGTVASSSTAVIVSARMAPEEIIMDRPFLFVVRHNPTGTVLFMGQVMEP

  • Molecular Weight

    Approximately 48 kDa, based on SDS-PAGE under reducing conditions.

  • Glycosylation

    Yes

  • Purity

    ≥ 95%, as determined by reducing SDS-PAGE.

Product Properties

Appearance

Lyophilized powder.

Formulation

1.Lyophilized from a 0.22 μm filtered solution of 4 mM HCl.
2.Lyophilized from a 0.22 μm filtered solution of 20 mM HAC-NaAC, 150 mM NaCl, pH 5.5.
Please refer to the lot-specific COA for specific buffer information.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Storage & Stability

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US;may vary elsewhere.

Calculators

Reconstitution Calculator

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) Volume (to add to vial)
=
Mass (in vial) Mass (in vial)
÷
Desired Reconstitution Concentration Desired Reconstitution Concentration
Dilution Calculator

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

Concentration (start) Concentration (start)
×
Volume (start) Volume (start)
=
Concentration (final) Concentration (final)
×
Volume (final) Volume (final)
The Specific Activity Calculator Equation
  • Specific Activity (Unit/mg)
  • Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) Specific Activity (Unit/mg)
Unit/mg
= 106 ÷
Biological Activity (ED50) Biological Activity (ED50)
106 ÷
ng/mL
MOQ
Minimum order quantity
100 mg

Get Quote In-stock

Other size
Get Quote
Please select quantity
Amount: USD 0.00

Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     ≥ 95%, as determined by reducing SDS-PAGE.

  • Serpin E1 Protein, Human (HEK293, His) Product Image
    1/16
  • Experimental Validation Results for Serpin E1 Protein, Human (HEK293, His)
  • Serpin E1 Protein, Human (HEK293, His) Product Image