PSAP/Prosaposin Protein, Human (HEK293, His)

Frizzled-7 protein is a transmembrane receptor that plays a crucial role in the Wnt signaling pathway. It is involved in cellular processes such as cell proliferation, differentiation, and migration. PSAP/Prosaposin Protein, Human (HEK293, His) is the recombinant human-derived PSAP/Prosaposin protein, expressed by HEK293 , with C-10*His labeled tag.

The PSAP/Prosaposin Protein, encoded by this gene, is a highly conserved preproprotein that undergoes proteolytic processing to yield four main cleavage products—saposins A, B, C, and D. Each domain of the precursor protein, approximately 80 amino acid residues long, exhibits nearly identical placement of cysteine residues and glycosylation sites. Saposins A-D primarily localize to the lysosomal compartment, where they play a crucial role in facilitating the catabolism of glycosphingolipids with short oligosaccharide groups. The precursor protein exists in both secretory and integral membrane forms and possesses neurotrophic activities. Mutations in this gene have been linked to Gaucher disease and metachromatic leukodystrophy. Alternative splicing yields multiple transcript variants, with at least one encoding an isoform that undergoes proteolytic processing. The gene displays ubiquitous expression, with elevated levels detected in the spleen (RPKM 523.6), gall bladder (RPKM 491.5), and 25 other tissues, emphasizing its essential role across various physiological contexts in multiple organs.

Human

HEK293

C-10*His

NP_002769.1 (G17-N524)

5660  [NCBI]

Full Length of Mature Protein

GPVLGLKECTRGSAVWCQNVKTASDCGAVKHCLQTVWNKPTVKSLPCDICKDVVTAAGDMLKDNATEEEILVYLEKTCDWLPKPNMSASCKEIVDSYLPVILDIIKGEMSRPGEVCSALNLCESLQKHLAELNHQKQLESNKIPELDMTEVVAPFMANIPLLLYPQDGPRSKPQPKDNGDVCQDCIQMVTDIQTAVRTNSTFVQALVEHVKEECDRLGPGMADICKNYISQYSEIAIQMMMHMQPKEICALVGFCDEVKEMPMQTLVPAKVASKNVIPALELVEPIKKHEVPAKSDVYCEVCEFLVKEVTKLIDNNKTEKEILDAFDKMCSKLPKSLSEECQEVVDTYGSSILSILLEEVSPELVCSMLHLCSGTRLPALTVHVTQPKDGGFCEVCKKLVGYLDRNLEKNSTKQEILAALEKGCSFLPDPYQKQCDQFVAEYEPVLIEILVEVMDPSFVCLKIGACPSAHKPLLGTEKCIWGPSYWCQNTETAAQCNAVEHCKRHVWN

60-85 kDa

• 
  ≥ 95%, as determined by reducing SDS-PAGE.

Lyophilized powder.

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.