RIP1/RIP3/MLKL activator 1
Based on 7 publication(s) in Google Scholar
RIP1/RIP3/MLKL activator 1 (Compound 6i) is a potent anti-glioma agent. RIP1/RIP3/MLKL activator 1 induces necroptosis through RIP1/RIP3/MLKL pathway. RIP1/RIP3/MLKL activator 1 exerts acceptable BBB permeability.
For research use only. We do not sell to patients.
- Purity: 97.0%
- CAS No.: 2682850-41-3
- Formula: C43H56N4O3
- Molecular Weight:676.93
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Storage:
4°C, stored under nitrogen
* In solvent : -80°C, 6 months; -20°C, 1 month (stored under nitrogen)
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
Publications Citing Use of MedChemExpress (MCE) RIP1/RIP3/MLKL activator 1
More- Bone Res. 2024 Aug 27;12(1):47. [Abstract]
- J Adv Res. 2026 Mar 12:S2090-1232(26)00234-1. [Abstract]
- Cell Rep Med. 2026 Feb 18:102630. [Abstract]
- Adv Healthc Mater. 2024 Apr;13(9):e2303337. [Abstract]
- Int J Biol Macromol. 2024 Sep 19:135835. [Abstract]
- Ecotoxicol Environ Saf. 2026 Jun 5:322:120317. [Abstract]
- Front Biosci (Landmark Ed). 2025 Jan 16;30(1):26186. [Abstract]
Customer Validation & Images
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Cell Proliferation/Viability Assay
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Cell Imaging/Staining
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WB
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IF
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RT-PCR
Biological Activity
RIP1, RIP3, MLKL[1]
RIP1/RIP3/MLKL activator 1 (Compound 6i) (96 h) shows antiproliferative activities in human glioma cell lines[1].
RIP1/RIP3/MLKL activator 1 (0-4 μM, 0-72 h) exhibits remarkable antiproliferative activity for U251 cells in a time- and concentration-dependent manner[1].
RIP1/RIP3/MLKL activator 1 (10 μM, 0-72 h) shows acceptable stability[1].
RIP1/RIP3/MLKL activator 1 (0-2 μM, 24 h) effectively inhibits the migration of U251 cells[1].
RIP1/RIP3/MLKL activator 1 induces necroptosis through RIP1/RIP3/MLKL pathway, and induces mitochondrial depolarization in U251 cells[1].
RIP1/RIP3/MLKL activator 1 could not induce apoptosis in U251 cells[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:A172, LN229, U87, U251 and L02 cell lines
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Concentration:0-4 µM for U251 cells
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Incubation Time:96 h; 24, 48, and 72 h for U251 cells
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Result:Showed antiproliferative activity with IC50 values of 3.03 ± 0.70, 1.78 ± 0.79, 1.22 ± 0.89, 0.94 ± 0.45, and 0.99 ± 0.46 µM against A172, LN229, U87, U251 and L02 cells, respectively. Time- and concentration-dependently inhibited the growth in U251 cells.
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Cell Line:U251
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Concentration:0, 0.5, 1, 2, and 4 µM
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Incubation Time:24 or 48 h
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Result:Concentration-dependently upregulated the expression of p-RIP1, RIP1, p-RIP3, RIP3, p-MLKL, and MLKL at 24 or 48 h.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:Zebrafish wide-type AB strain; 200 CM-DiI labeled U251 cells were transplanted into yolk sac of each wild-type zebrafish embryos at 2 dpf (2 days postfertilization)[1]
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Dosage:2.50 ng/tail
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Administration:Microinjection; 48 h
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Result:Remarkably reduced the U251 xenografts fluorescence intensity.
Chemical Information
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CAS No. 2682850-41-3
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Appearance Solid
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Molecular Weight 676.93
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Formula C43H56N4O3
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Color Orange to red
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SMILES
CC1=C(C(C=C2C1=CC=C3[C@]4(CC[C@]5(CC[C@@](C(NCC6=CN(N=N6)CC7=CC=C(C=C7)C(C)(C)C)=O)(CC5[C@@]4(CC[C@@]23C)C)C)C)C)=O)O
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
4°C, stored under nitrogen
* In solvent : -80°C, 6 months; -20°C, 1 month (stored under nitrogen)
Publications (7)
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Journal Impact Factor
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Most Recent
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Bone Res
The HOXC10/NOD1/ERK axis drives osteolytic bone metastasis of pan-KRAS-mutant lung cancer. [Abstract]2024 Aug 27;12(1):47. PMID: 39191757 -
J Adv Res
Restoring cardiolipin homeostasis mitigates cerebral ischemia-reperfusion injury by suppressing ATG5-mediated neuronal autophagy-dependent ferroptosis. [Abstract]2026 Mar 12:S2090-1232(26)00234-1. PMID: 41831680
RIP1/RIP3/MLKL activator 1 purchased from MedChemExpress. Usage Cited in: J Adv Res. 2026 Mar 12:S2090-1232(26)00234-1. [Abstract]
RIP1/RIP3/MLKL activator 1 (Necroptosis, IC50 concentration). Neuro-2a cells were treated with inducers of different types of cell death with the IC50 concentration, respectively, and different concentrations of SS-31 (0.1, 0.2, 0.5, 1, 2, 5, 10 μg/ml) at the same time. Cell viability was measured by CCK-8 assay after 24 h.
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Cell Rep Med
HIF-activated priming of TRAIL-induced cell death determines epigenetic vulnerability in kidney cancer. [Abstract]2026 Feb 18:102630. PMID: 41713410
RIP1/RIP3/MLKL activator 1 purchased from MedChemExpress. Usage Cited in: Cell Rep Med. 2026 Feb 18:102630. [Abstract]
Representative images of death of RCC10 cells pre-treated with vehicle or Necrostatin-1 for 30 min, followed by 3 days of co-treatment with RIP1/RIP3/MLKL activator 1 (Compound 6i, 0.5 μM). Scale bar, 100 μm.
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Adv Healthc Mater
Nanointegrative Glycoengineering-Activated Necroptosis of Triple Negative Breast Cancer Stem Cells Enables Self-Amplifiable Immunotherapy for Systemic Tumor Rejection. [Abstract]2024 Apr;13(9):e2303337. PMID: 38154036 -
Int J Biol Macromol
Tremella aurantialba polysaccharides alleviate ulcerative colitis in mice by improving intestinal barrier via modulating gut microbiota and inhibiting ferroptosis. [Abstract]2024 Sep 19:135835. PMID: 39306158
RIP1/RIP3/MLKL activator 1 purchased from MedChemExpress. Usage Cited in: Int J Biol Macromol. 2024 Sep 19:135835. [Abstract]
Caco 2 cells were treated with pretreated with different concentrations of TA 2-1 before RIP1/RIP3/MLKL activator 1 (2 μM) stimulation for 3 h. After 24 h, the cell viability was detected with microplate reader at 450 nm.
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Ecotoxicol Environ Saf
Hydramethylnon-induced pulmonary toxicity associated with necroptosis signaling and mitochondrial dysfunction in human bronchial epithelial cells. [Abstract]2026 Jun 5:322:120317. PMID: 42248040
RIP1/RIP3/MLKL activator 1 purchased from MedChemExpress. Usage Cited in: Ecotoxicol Environ Saf. 2026 Jun 5:322:120317. [Abstract]
BEAS-2B and 16HBE14o cells treated with the RIP1/RIP3/MLKL activator 1 (Nec inducer, 1 µM) for the indicated time periods. Immunoblotting analyses performed with the indicated antibodies.
RIP1/RIP3/MLKL activator 1 purchased from MedChemExpress. Usage Cited in: Ecotoxicol Environ Saf. 2026 Jun 5:322:120317. [Abstract]
BEAS-2B and 16HBE14o cells treated with the RIP1/RIP3/MLKL activator 1 (Nec inducer) for the indicated concentrations for 24 h. Immunofluorescence analyses carried out using anti-TOM20 antibody or DAPI.
RIP1/RIP3/MLKL activator 1 purchased from MedChemExpress. Usage Cited in: Ecotoxicol Environ Saf. 2026 Jun 5:322:120317. [Abstract]
RIP1/RIP3/MLKL activator 1 (Nec inducer, 1-2 μM, 24 h)Relative copy number of mitochondrial DNA analyzed by qPCR.
RIP1/RIP3/MLKL activator 1 purchased from MedChemExpress. Usage Cited in: Ecotoxicol Environ Saf. 2026 Jun 5:322:120317. [Abstract]
RIP1/RIP3/MLKL activator 1 (Nec inducer, 1-2 μM; 24 h) was used to activate necroptosis signaling in HBECs, the treatment successfully decreased intracellular ATP levels.
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Front Biosci (Landmark Ed)
Inhibition of p70 Ribosomal S6K1 Protects the Myocardium against Ischemia/Reperfusion-Induced Necrosis through Downregulation of RIP3. [Abstract]2025 Jan 16;30(1):26186. PMID: 39862085
Solvent & Solubility
DMSO : 100 mg/mL (147.73 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (3.69 mM); Suspended solution
This protocol yields a suspended solution of ≥ 2.5 mg/mL (saturation unknown). Suspended solution can be used for oral and intraperitoneal injection.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL. * In solvent : -80°C, 6 months; -20°C, 1 month (stored under nitrogen)
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
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Data Sheet (280 KB)
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SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
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Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
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| DMSO | 1 mM | 1.4773 mL | 7.3863 mL | 14.7726 mL | 36.9314 mL |
| 5 mM | 0.2955 mL | 1.4773 mL | 2.9545 mL | 7.3863 mL | |
| 10 mM | 0.1477 mL | 0.7386 mL | 1.4773 mL | 3.6931 mL | |
| 15 mM | 0.0985 mL | 0.4924 mL | 0.9848 mL | 2.4621 mL | |
| 20 mM | 0.0739 mL | 0.3693 mL | 0.7386 mL | 1.8466 mL | |
| 25 mM | 0.0591 mL | 0.2955 mL | 0.5909 mL | 1.4773 mL | |
| 30 mM | 0.0492 mL | 0.2462 mL | 0.4924 mL | 1.2310 mL | |
| 40 mM | 0.0369 mL | 0.1847 mL | 0.3693 mL | 0.9233 mL | |
| 50 mM | 0.0295 mL | 0.1477 mL | 0.2955 mL | 0.7386 mL | |
| 60 mM | 0.0246 mL | 0.1231 mL | 0.2462 mL | 0.6155 mL | |
| 80 mM | 0.0185 mL | 0.0923 mL | 0.1847 mL | 0.4616 mL | |
| 100 mM | 0.0148 mL | 0.0739 mL | 0.1477 mL | 0.3693 mL |
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