1. Immunology/Inflammation
  2. NOD-like Receptor (NLR)
  3. Ruscogenin

Ruscogenin, an important steroid sapogenin derived from Ophiopogon japonicus, attenuates cerebral ischemia-induced blood-brain barrier dysfunction by suppressing TXNIP/NLRP3 inflammasome activation and the MAPK pathway. Ruscogenin exerts significant anti-inflammatory and anti-thrombotic activities. Ruscogenin has orally bioactivity.

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Ruscogenin Chemical Structure

Ruscogenin Chemical Structure

CAS No. : 472-11-7

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10 mM * 1 mL in DMSO USD 108 In-stock
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5 mg USD 99 In-stock
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Based on 2 publication(s) in Google Scholar

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Description

Ruscogenin, an important steroid sapogenin derived from Ophiopogon japonicus, attenuates cerebral ischemia-induced blood-brain barrier dysfunction by suppressing TXNIP/NLRP3 inflammasome activation and the MAPK pathway. Ruscogenin exerts significant anti-inflammatory and anti-thrombotic activities. Ruscogenin has orally bioactivity[1][2].

IC50 & Target

NLRP3

 

In Vitro

Ruscogenin (0.01-40 μM; 24 h) increases the cell viability in bEnd.3 cells subjected to OGD/R[1].
Ruscogenin (0.01-10 μM; 24 h) reverts the endothelial barrier leakage, inhibits the expression of IL-Iβ and Caspase-1, and modulats the TXNIP/NLRP3 pathway in bEnd.3 cells subjected to OGD/R[1].
Ruscogenin (0.01-10 μM; 4 h) inhibits the production of ROS, and regulated the MAPK Pathway in bEnd.3 Cells subjected to OGD/R[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Viability Assay[1]

Cell Line: bEnd.3 cell
Concentration: 0.01 μM, 0.1 μM, 1 μM, 10 μM, 20 μM, 40 μM
Incubation Time: 24 h
Result: Showed that the cell viability reduction in OGD/R-induced bEnd.3 cell was significantly recovered.

Immunofluorescence[1]

Cell Line: bEnd.3 cell
Concentration: 0.1 μM, 1 μM, 10 μM
Incubation Time: 24 h
Result: Demonstrated increase in the TEER value and inhibition the sodium fluorescein permeability.

Western Blot Analysis[1]

Cell Line: bEnd.3 cell
Concentration: 0.01 μM, 0.1 μM, 1 μM, 10 μM, 20 μM, 40 μM
Incubation Time: 24 h
Result: Demonstrated downregulation of the expression of IL-1β and Caspase-1 proteins, and inhibition in the expressions of NLRP3 and TXNIP.

Immunofluorescence[1]

Cell Line: bEnd.3 cell
Concentration: 0.01 μM, 0.1 μM, 1 μM, 10 μM, 20 μM, 40 μM
Incubation Time: 4 h
Result: Demonstrated reduction in the production of ROS.
In Vivo

Ruscogenin (10 mg/kg; i.g.; 1 time) improves the MCAO/R-induced brain tissue injury and inhibits the expression of IL-1β and Caspase-1 and modulats the TXNIP/NLRP3[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: MCAO/R Mice[1]
Dosage: 10mg/kg
Administration: Oral Gavage (i.g.)
Result: Showed smaller infarct size, ameliorating histopathological damage by decreasing the cell loss, and significant increase in CBF (cerebral blood flow) compared to the model group.
Resulted inhibiting the expression of IL-1β and Caspase-1, NLRP3 and TXNIP compared to the model group.
Molecular Weight

430.62

Formula

C27H42O4

CAS No.
Appearance

Solid

Color

White to off-white

SMILES

C[C@@]12[C@]([C@@H]3C)([H])[C@](O[C@]34CC[C@@H](C)CO4)([H])C[C@@]1([H])[C@@](CC=C(C[C@@H](O)C5)[C@@]6([C@@H]5O)C)([H])[C@]6([H])CC2

Structure Classification
Initial Source
Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Purity & Documentation

Purity: 99.59%

References
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