1. Membrane Transporter/Ion Channel Neuronal Signaling
  2. GABA Receptor
  3. SCS

SCS  (Synonyms: Salicylidene salicylhydrazide)

Cat. No.: HY-103528
Handling Instructions

SCS (Salicylidene salicylhydrazide) is a potent, allosteric and selective inhibitor of β1-containing GABAA receptors with an IC50 of 32 nM against α2β1γ1θ by VIPR measurement. SCS is also a chelator of metal ions.

For research use only. We do not sell to patients.

SCS Chemical Structure

SCS Chemical Structure

CAS No. : 3232-36-8

Size Price Stock
5 mg USD 102 Ask For Quote & Lead Time

* Please select Quantity before adding items.

This product is a controlled substance and not for sale in your territory.

Top Publications Citing Use of Products
  • Biological Activity

  • Purity & Documentation

  • References

  • Customer Review

Description

SCS (Salicylidene salicylhydrazide) is a potent, allosteric and selective inhibitor of β1-containing GABAA receptors with an IC50 of 32 nM against α2β1γ1θ by VIPR measurement. SCS is also a chelator of metal ions[1].

IC50 & Target

IC50: 32 nM (α2β1γ1θ; by VIPR measurement)
IC50: 4.5 nM (α2β1γ1θ), 5.3 nM (α2β1γ1), 7.9 nM (α1β1γ2s) (Measured by using whole-cell patch clamp)[1]

In Vitro

SCS (0.1 nM-3 μM) produces a concentration-dependent inhibition of GABA EC20 currents recorded from Ltk- cells expressing α2β1γ1θ, α2β1γ1 and α1β1γ2s receptors compared with α2β3γ2s and α1β2γ2s receptors upon which SCS has no effect[1].
Inhibition by SCS is not voltage or use dependent[1].
Structural determinants necessary for the inhibition of GABAA receptors by SCS are located within the region arginine 238 and glycine 335 of the β1 subunit. T255 and I308 of the β1 subunit are required for inhibition by SCS[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

In Vivo

SCS (Salicylidene salicylhydrazide; 500-1000 mg/kg, i.p. or 800-1000 mg/kg, oral) produces abdominal constrictions in mice[2].
SCS (10-75 mg/kg; i.p.; once) shows antinociceptive activity against tonic, phasic and Capsaicin (HY-10448) nociception in mice[2].
SCS (10-75 mg/kg; i.p.; once) shows anti-inflammatory activity in mice[2]. SCS (50 and 75 mg/kg; i.p.; once) shows antinociceptive activity against neuropathic nociception[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: BALB/c mice; tonic, phasic and Capsaicin (HY-10448) nociception model[2]
Dosage: 10, 25, 50, and 75 mg/kg
Administration: IP, single dose
Result: Produced a significant protection on tonic, phasic and capsaicin nociception in a dose-dependent manner.
Animal Model: BALB/c mice, Oxaliplatin (HY-17371)-induced neuropathic nociception model[2]
Dosage: 50 and 75 mg/kg
Administration: IP, single dose
Result: Significantly attenuated the paw withdrawal threshold changes associated with Oxaliplatin. Significantly increased the percent antinociception during 30-120 min.
Clinical Trial
Molecular Weight

256.26

Formula

C14H12N2O3

CAS No.
SMILES

O=C(N/N=C/C1=CC=CC=C1O)C2=CC=CC=C2O

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Please store the product under the recommended conditions in the Certificate of Analysis.

Solvent & Solubility
In Vitro: 

DMSO : 50 mg/mL (195.11 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 3.9023 mL 19.5114 mL 39.0229 mL
5 mM 0.7805 mL 3.9023 mL 7.8046 mL
View the Complete Stock Solution Preparation Table
  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
Purity & Documentation
References

Complete Stock Solution Preparation Table

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 3.9023 mL 19.5114 mL 39.0229 mL 97.5572 mL
5 mM 0.7805 mL 3.9023 mL 7.8046 mL 19.5114 mL
10 mM 0.3902 mL 1.9511 mL 3.9023 mL 9.7557 mL
15 mM 0.2602 mL 1.3008 mL 2.6015 mL 6.5038 mL
20 mM 0.1951 mL 0.9756 mL 1.9511 mL 4.8779 mL
25 mM 0.1561 mL 0.7805 mL 1.5609 mL 3.9023 mL
30 mM 0.1301 mL 0.6504 mL 1.3008 mL 3.2519 mL
40 mM 0.0976 mL 0.4878 mL 0.9756 mL 2.4389 mL
50 mM 0.0780 mL 0.3902 mL 0.7805 mL 1.9511 mL
60 mM 0.0650 mL 0.3252 mL 0.6504 mL 1.6260 mL
80 mM 0.0488 mL 0.2439 mL 0.4878 mL 1.2195 mL
100 mM 0.0390 mL 0.1951 mL 0.3902 mL 0.9756 mL
  • No file chosen (Maximum size is: 1024 Kb)
  • If you have published this work, please enter the PubMed ID.
  • Your name will appear on the site.
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Your Recently Viewed Products:

Inquiry Online

Your information is safe with us. * Required Fields.

Product Name

 

Salutation

Applicant Name *

 

Email Address *

Phone Number *

 

Organization Name *

Department *

 

Requested quantity *

Country or Region *

     

Remarks

Bulk Inquiry

Inquiry Information

Product Name:
SCS
Cat. No.:
HY-103528
Quantity:
MCE Japan Authorized Agent: