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endopeptidase substrate

" in MedChemExpress (MCE) Product Catalog:

By Targets:	Prolyl Endopeptidase (PREP) (3) Bacterial (3) Biochemical Assay Reagents (2) FAP (1) Fluorescent Dye (3)
By Research Areas:	Cancer (2) Infection (3)

Inhibitors & Agonists

Fluorescent Dyes

Peptides

Targets Recommended: MARCKS Legumain Prolyl Endopeptidase (PREP) Neprilysin

Cat. No.	Product Name	Target	Research Areas	Chemical Structure

HY-D1670

Z-Gly-Pro-AMC 3 Publications Verification	Fluorescent Dye	Others
Z-Gly-Pro-AMC is a fluorogenic substrate. Z-Gly-Pro-AMC is hydrolyzed by prolyl endopeptidase to generate highly fluorescent 7-Amino-4-methylcoumarin (HY-D0027). (λ_ex=380 nm, λ_em=465 nm) .

HY-145983

Suc-Gly-Pro-AMC 1 Publications Verification	FAP Prolyl Endopeptidase (PREP)	Cancer
Suc-Gly-Pro-AMC is a fluorescent substrate. Suc-Gly-Pro-AMC is a fibroblast activation protein (FAP) specific substrate. Suc-Gly-Pro-AMC reacts with recombinant porcine prolyl oligopeptidase. Suc-Gly-Pro-AMC can be used to study the activity of FAP, prolyl endopeptidase (PREP). Suc-Gly-Pro-AMC is used in glioma research .

HY-P3205

Lysyl endopeptidase, Achromobacter sp 1 Publications Verification Lys-C	Biochemical Assay Reagents	Others
Lysyl endopeptidase, Achromobacter sp (Lys-C) catalyzes carboxyl oxygen exchange reaction. Lysyl endopeptidase has higher substrate binding affinities and higher catalytic rates at the acidic pHs than at the alkaline pHs .

HY-P4490

Suc-Ala-Pro-pNA 1 Publications Verification	Prolyl Endopeptidase (PREP)	Others
Suc-Ala-Pro-pNA is a peptide that serves as a substrate to detect the activity and stability of prolyl endopeptidase (PEP) .

HY-137220

Z-Gly-Pro-pNA	Prolyl Endopeptidase (PREP)	Others
Z-Gly-Pro-pNA is a substrate for measuring prolyl endopeptidase (PEP) inhibitory activity .

HY-P4455

Boc-AAG-pNA	Bacterial	Infection
Boc-AAG-pNA is a glycine endopeptidase substrate. Boc-AAG-pNA can be used to test the amidase activity glycine endopeptidase .

HY-137337

Suc-Ala-Ala-Phe-AMC	Fluorescent Dye	Others
Suc-Ala-Ala-Phe-AMC is a fluorogenic chymotrypsin substrate, can be hydrolyzed by endopeptidase. Suc-Ala-Ala-Phe-AMC has been used in both in vivo assays of the acrosome reaction and in vitro enzyme assays .

HY-P4482

Suc-AAPE-pNA	Bacterial	Infection
Suc-AAPE-pNA is a chromogenic substrate of glutamyl endopeptidase. Suc-AAPE-pNA can be used to test glutamyl endopeptidase activity .

HY-114318

Bz-DL-Arg-βNA hydrochloride	Bacterial	Infection
Bz-DL-Arg-βNA hydrochloride is a substrate for endopeptidases but is not hydrolyzed by bacterial dipeptidyl peptidase DPP-III .

HY-P4448

Ac-Gly-Pro-AFC	Fluorescent Dye	Others
Ac-Gly-Pro-AFC is a fluorogenic substrate for the determination of fibroblast activation protein (FAP) endopeptidase activity .

HY-E71335

γ-D-Glutamyl-meso-diaminopimelate peptidase	Biochemical Assay Reagents	Others
γ-D-Glutamyl-meso-diaminopimelate peptidase (EC 3.4.19.11) is a 45-kDa metallopeptidase from Bacillus sphaericus, the substrates being components of the bacterial spore wall. A member of peptidase family M14 (carboxypeptidase A family) . Endopeptidase II has similar activity, but differs in cellular location, molecular mass and catalytic mechanism.

Cat. No.	Product Name	Type

HY-D1670

Z-Gly-Pro-AMC 3 Publications Verification	Fluorescent Dyes
Z-Gly-Pro-AMC is a fluorogenic substrate. Z-Gly-Pro-AMC is hydrolyzed by prolyl endopeptidase to generate highly fluorescent 7-Amino-4-methylcoumarin (HY-D0027). (λ_ex=380 nm, λ_em=465 nm) .

Cat. No.	Product Name	Target	Research Area

HY-P4490

Suc-Ala-Pro-pNA 1 Publications Verification	Prolyl Endopeptidase (PREP)	Others
Suc-Ala-Pro-pNA is a peptide that serves as a substrate to detect the activity and stability of prolyl endopeptidase (PEP) .

HY-P4516

Z-Phe-Leu-Glu-pNA	Peptides	Cancer
Z-Phe-Leu-Glu-pNA is a chromogenic substrate for glutamyl endopeptidases. Z-Phe-Leu-Glu-pNA has been used as a synthetic substrate in the study of proteolytic enzymes, including trypsin treatment, subtilisin and chymotrypsin. Z-Phe-Leu-Glu-pNA plays an important role in hormone disorders such as prostate cancer and breast cancer .

HY-P4455

Boc-AAG-pNA	Bacterial	Infection
Boc-AAG-pNA is a glycine endopeptidase substrate. Boc-AAG-pNA can be used to test the amidase activity glycine endopeptidase .

HY-137337

Suc-Ala-Ala-Phe-AMC	Fluorescent Dye	Others
Suc-Ala-Ala-Phe-AMC is a fluorogenic chymotrypsin substrate, can be hydrolyzed by endopeptidase. Suc-Ala-Ala-Phe-AMC has been used in both in vivo assays of the acrosome reaction and in vitro enzyme assays .

HY-P4482

Suc-AAPE-pNA	Bacterial	Infection
Suc-AAPE-pNA is a chromogenic substrate of glutamyl endopeptidase. Suc-AAPE-pNA can be used to test glutamyl endopeptidase activity .

HY-P4498

Z-Ala-Pro-pNA	Peptides	Others
Z-Ala-Pro-pNA is an endopeptidase substrate and can be used to detect the activity of this enzyme .

HY-P4448

Ac-Gly-Pro-AFC	Fluorescent Dye	Others
Ac-Gly-Pro-AFC is a fluorogenic substrate for the determination of fibroblast activation protein (FAP) endopeptidase activity .

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BODIPY 493/503 purchased from MedChemExpress. Usage Cited in: Nat Commun. 2025 Feb 1;16(1):1241. [Abstract]

MedchemExpress Validation 01

Western blot analysis of extracts from THP-1(lane 2(20μg), Jurkat (lane 3(20μg) and NIH3T3(lane 4(20μg) using FOXO1A (HY-P80132) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature. Western blot analysis of extracts from THP-1(lane 2(20μg), Jurkat (lane 3(20μg) and NIH3T3(lane 4(20μg) using FOXO1A (HY-P80132) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature. Western blot analysis of extracts from THP-1(lane 2(20μg), Jurkat (lane 3(20μg) and NIH3T3(lane 4(20μg) using FOXO1A (HY-P80132) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

BODIPY 493/503 purchased from MedChemExpress. Usage Cited in: Nat Commun. 2025 Feb 1;16(1):1241. [Abstract]

MedchemExpress Validation 01

MedchemExpress Validation 03

Western blot analysis of extracts from THP-1(lane 2(20μg), Jurkat (lane 3(20μg) and NIH3T3(lane 4(20μg) using FOXO1A (HY-P80132) Rabbit mAb. Proteins were transferred

MedchemExpress Validation 04

MedchemExpress Validation

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