Lysyl endopeptidase, Achromobacter sp
Based on 1 publication(s) in Google Scholar
Lysyl endopeptidase, Achromobacter sp (Lys-C) catalyzes carboxyl oxygen exchange reaction. Lysyl endopeptidase has higher substrate binding affinities and higher catalytic rates at the acidic pHs than at the alkaline pHs.
For research use only. We do not sell to patients.
- CAS No.: 123175-82-6
-
Storage:
Please store the product under the recommended conditions in the Certificate of Analysis.
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
-
Biological Activity
-
Technical Parameters
-
Chemical Information
- Purity & Documentation
- References
-
Help & FAQs
Publications Citing Use of MedChemExpress (MCE) Lysyl endopeptidase, Achromobacter sp
More
Biological Activity
Product Information
Derived from Achromobacter, it is recombinantly expressed in Escherichia coli. This product is a serine protease that can highly specifically hydrolyze amides, esters, and peptide bonds on the carboxyl side of lysine. The optimal reaction pH is 9.0-9.5, and the optimal reaction temperature is 30-37°C. It can still maintain high enzyme activity in the presence of 4M urea or 0.1% SDS.
The storage solution is 20 mM Tris-HCl, pH 8.0.
Instructions
Protein digestion, the recommended digestion ratio is 1:50-1:200 (w/w, enzyme: protein), and digestion is carried out at 37°C overnight.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
3.4.21.50
≥3.0 AU/mg soild
One activity unit is defined as the amount of enzyme that catalyze the formation of 1 μmol AC-LyspNA in 1 min at 37℃ in Tris-HCl buffer at 25 mmol/L, pH 9.0
Technical Parameters
-
Source
E.coli
Chemical Information
-
CAS No. 123175-82-6
-
Appearance Liquid
-
Color Colorless to light yellow
-
SMILES
[Lysyl endopeptidase, Achromobacter sp]
-
Synonyms
Lys-C
-
Shipping
Room temperature in continental US; may vary elsewhere.
-
Storage
Please store the product under the recommended conditions in the Certificate of Analysis.
Publications (1)
-
Journal Impact Factor
-
Most Recent
Purity & Documentation
-
Data Sheet (266 KB)
-
SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
-
Handling Instructions (2659 KB)
References
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Lysyl endopeptidase, Achromobacter sp Related Classifications
HY-P3205 Related Classifications
Powered by Bioz