Cat. No.: HY-N0117A

(Z)-Indirubin

(Synonyms: (Z)-Couroupitine B; (Z)-Indigo red; (Z)-Indigopurpurin)

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(Z)-Indirubin is an isomer of Indirubin (HY-N0117). Indirubin is a bis-indole alkaloid and has emarkable anticancer activity against chronic myelocytic leukemia.

For research use only. We do not sell to patients.

Purity: 99.95%
CAS No.: 906748-38-7
Formula: C₁₆H₁₀N₂O₂
Molecular Weight:262.26

Storage:

Powder -20°C, 3 years ; In solvent -80°C, 6 months , -20°C, 1 month

(Z)-Indirubin Chemical Structure

CAS No.: 906748-38-7

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Use of MCE Products

Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biological Activity
- Description
- Cellular Effect
Chemical Information
Solvent & Solubility
- In Vitro
Purity & Documentation
References
Help & FAQs
Classifications
Bioz

Biological Activity

Description

(Z)-Indirubin is an isomer of Indirubin (HY-N0117). Indirubin is a bis-indole alkaloid and has emarkable anticancer activity against chronic myelocytic leukemia^[1].

Cellular Effect

Cell Line	Type	Value	Description	References
A549	IC₅₀	>10 μM Compound: Indirubin	Antiproliferative activity against human A549 cells assessed as cell growth inhibition measured after 48 hrs by CCK8 assay Antiproliferative activity against human A549 cells assessed as cell growth inhibition measured after 48 hrs by CCK8 assay	[PMID: 34624191]
A549	IC₅₀	>100 μM Compound: 4a	Antiproliferative activity against apoptosis resistant human A549 cells after 72 hrs by MTT assay Antiproliferative activity against apoptosis resistant human A549 cells after 72 hrs by MTT assay	[PMID: 26883150]
A549	IC₅₀	31 μM Compound: Indirubin	Inhibitory activity on the proliferation of Human lung carcinoma A549 cancer cells using MTT or sulforhodamine B assay Inhibitory activity on the proliferation of Human lung carcinoma A549 cancer cells using MTT or sulforhodamine B assay	[PMID: 15993584]
B16-F10	IC₅₀	0.2 μM Compound: 4a	Antiproliferative activity against apoptosis sensitive mouse B16F10 cells after 72 hrs by MTT assay Antiproliferative activity against apoptosis sensitive mouse B16F10 cells after 72 hrs by MTT assay	[PMID: 26883150]
Cancer cell lines	IC₅₀	>100 μM Compound: Indirubin	Inhibitory activity on the proliferation of Human colon carcinoma Co12 cancer cells using MTT or sulforhodamine B assay Inhibitory activity on the proliferation of Human colon carcinoma Co12 cancer cells using MTT or sulforhodamine B assay	[PMID: 15993584]
DU-145	IC₅₀	6.8 μM Compound: Indirubin	Cytotoxicity against human DU145 cells after 24 hrs by MTT assay Cytotoxicity against human DU145 cells after 24 hrs by MTT assay	[PMID: 20627564]
HCT-116	IC₅₀	19.52 μM Compound: Indirubin	Cytotoxicity against human HCT-116 cells assessed as inhibition of cell viability incubated for 48 hrs by CCK8 assay Cytotoxicity against human HCT-116 cells assessed as inhibition of cell viability incubated for 48 hrs by CCK8 assay	[PMID: 37801824]
HeLa	IC₅₀	>10 μM Compound: Indirubin	Antiproliferative activity against human HeLa cells assessed as cell growth inhibition measured after 48 hrs by CCK8 assay Antiproliferative activity against human HeLa cells assessed as cell growth inhibition measured after 48 hrs by CCK8 assay	[PMID: 34624191]
HeLa	IC₅₀	40 μM Compound: 1	Cytotoxicity against human HeLa cells assessed as cell growth inhibition measured after 24 hrs by SRB assay Cytotoxicity against human HeLa cells assessed as cell growth inhibition measured after 24 hrs by SRB assay	[PMID: 34161865]
HeLa	IC₅₀	40 μM Compound: 58	Antiproliferative activity against human HeLa cells assessed as inhibition of cell growth incubated for 24 hrs by SRB assay Antiproliferative activity against human HeLa cells assessed as inhibition of cell growth incubated for 24 hrs by SRB assay	[PMID: 36170798]
HepG2	IC₅₀	>10 μM Compound: Indirubin	Antiproliferative activity against human HepG2 cells assessed as cell growth inhibition measured after 48 hrs by CCK8 assay Antiproliferative activity against human HepG2 cells assessed as cell growth inhibition measured after 48 hrs by CCK8 assay	[PMID: 34624191]
HepG2	IC₅₀	>100 μM Compound: 1a	Cytotoxicity against human HepG2 cells assessed as cell growth inhibition after 24 hrs by alamar blue assay Cytotoxicity against human HepG2 cells assessed as cell growth inhibition after 24 hrs by alamar blue assay	[PMID: 28743492]
HL-60	IC₅₀	>100 μM Compound: Indirubin	Inhibitory activity on the proliferation of Human myeloid leukemia HL-60 cancer cells using MTT or sulforhodamine B assay Inhibitory activity on the proliferation of Human myeloid leukemia HL-60 cancer cells using MTT or sulforhodamine B assay	[PMID: 15993584]
Hs 683	IC₅₀	>100 μM Compound: 4a	Antiproliferative activity against apoptosis sensitive human Hs683 cells after 72 hrs by MTT assay Antiproliferative activity against apoptosis sensitive human Hs683 cells after 72 hrs by MTT assay	[PMID: 26883150]
HT-1080	IC₅₀	42.2 μM Compound: Indirubin	Inhibitory activity on the proliferation of Human fibrosarcoma HT1080 cancer cells using MTT or sulforhodamine B assay Inhibitory activity on the proliferation of Human fibrosarcoma HT1080 cancer cells using MTT or sulforhodamine B assay	[PMID: 15993584]
K562	IC₅₀	>10 μM Compound: Indirubin	Antiproliferative activity against human K562 cells assessed as cell growth inhibition measured after 48 hrs by CCK8 assay Antiproliferative activity against human K562 cells assessed as cell growth inhibition measured after 48 hrs by CCK8 assay	[PMID: 34624191]
K562	IC₅₀	>100 μM Compound: 7	Antiproliferative activity against human K562 cells after 72 hrs by CCK8 assay Antiproliferative activity against human K562 cells after 72 hrs by CCK8 assay	[PMID: 29678463]
K562/VCR	IC₅₀	>100 μM Compound: 7	Antiproliferative activity against human K562/VCR cells after 72 hrs by CCK8 assay Antiproliferative activity against human K562/VCR cells after 72 hrs by CCK8 assay	[PMID: 29678463]
LoVo	IC₅₀	>100 μM Compound: 4a	Antiproliferative activity against human LoVo cells after 72 hrs by MTT assay Antiproliferative activity against human LoVo cells after 72 hrs by MTT assay	[PMID: 26883150]
LoVo	IC₅₀	2.2 μM Compound: Indirubin	Cytotoxicity against human LoVo cells after 24 hrs by MTT assay Cytotoxicity against human LoVo cells after 24 hrs by MTT assay	[PMID: 20627564]
MCF7	IC₅₀	>10 μM Compound: Indirubin	Antiproliferative activity against human MCF7 cells assessed as cell growth inhibition measured after 48 hrs by CCK8 assay Antiproliferative activity against human MCF7 cells assessed as cell growth inhibition measured after 48 hrs by CCK8 assay	[PMID: 34624191]
MCF7	IC₅₀	>100 μM Compound: 4a	Antiproliferative activity against apoptosis sensitive human MCF7 cells after 72 hrs by MTT assay Antiproliferative activity against apoptosis sensitive human MCF7 cells after 72 hrs by MTT assay	[PMID: 26883150]
MCF7	IC₅₀	4 μM Compound: Indirubin	Antiproliferative activity against human MCF7 cells after 3 days by SRB assay Antiproliferative activity against human MCF7 cells after 3 days by SRB assay	[PMID: 24326279]
MCF7	IC₅₀	6.7 μM Compound: Indirubin	Cytotoxicity against human MCF7 cells after 24 hrs by MTT assay Cytotoxicity against human MCF7 cells after 24 hrs by MTT assay	[PMID: 20627564]
MCF7	IC₅₀	9.91 μM Compound: 1; 4a	Antiproliferative activity against human MCF7 cells after 3 days by SRB assay Antiproliferative activity against human MCF7 cells after 3 days by SRB assay	[PMID: 28557430]
MV4-11	IC₅₀	>10 μM Compound: 1	Antiproliferative activity against wild type FLT3 expressing human MV411 cells after 72 hrs by WST8 assay Antiproliferative activity against wild type FLT3 expressing human MV411 cells after 72 hrs by WST8 assay	[PMID: 20153646]
Oocyte	IC₅₀	10 μM Compound: 1	Inhibition of starfish oocyte CDK1/Cyclin B using histone H1 as substrate incubated for 10 mins in presence of [gamma-32P]ATP by scintillation counter analysis Inhibition of starfish oocyte CDK1/Cyclin B using histone H1 as substrate incubated for 10 mins in presence of [gamma-32P]ATP by scintillation counter analysis	[PMID: 34161865]
Oocyte	IC₅₀	10 μM Compound: Indirubin	Inhibitory activity against cyclin dependent kinase 1-cyclinB from starfish (Marthasterias glacialis) oocytes Inhibitory activity against cyclin dependent kinase 1-cyclinB from starfish (Marthasterias glacialis) oocytes	[PMID: 14761195]
Oocyte	IC₅₀	6.5 μM Compound: 4	Inhibition of Cyclin-dependent kinase 1-cyclin B of M-phase Marthasterias glacialis oocytes Inhibition of Cyclin-dependent kinase 1-cyclin B of M-phase Marthasterias glacialis oocytes	[PMID: 15163202]
PC-3	IC₅₀	>100 μM Compound: 4a	Antiproliferative activity against apoptosis sensitive human PC3 cells after 72 hrs by MTT assay Antiproliferative activity against apoptosis sensitive human PC3 cells after 72 hrs by MTT assay	[PMID: 26883150]
RAW264.7	IC₅₀	6.1 μM Compound: Indirubin	Cytotoxicity against mouse RAW264.7 cells after 24 hrs by MTT assay Cytotoxicity against mouse RAW264.7 cells after 24 hrs by MTT assay	[PMID: 20627564]
RS4-11	IC₅₀	>10 μM Compound: 1	Antiproliferative activity against human RS4:11 cells having FLT3-ITD mutation after 72 hrs by WST8 assay Antiproliferative activity against human RS4:11 cells having FLT3-ITD mutation after 72 hrs by WST8 assay	[PMID: 20153646]
SK-MEL-28	IC₅₀	>100 μM Compound: 4a	Antiproliferative activity against apoptosis resistant human SK-MEL-28 cells after 72 hrs by MTT assay Antiproliferative activity against apoptosis resistant human SK-MEL-28 cells after 72 hrs by MTT assay	[PMID: 26883150]
U-118-MG	IC₅₀	12.5 μM Compound: 1	Cytotoxicity against human U-118-MG cells assessed as cell growth inhibition measured after 24 hrs by MTT assay Cytotoxicity against human U-118-MG cells assessed as cell growth inhibition measured after 24 hrs by MTT assay	[PMID: 34161865]
U-373MG ATCC	IC₅₀	>100 μM Compound: 4a	Antiproliferative activity against apoptosis resistant human U373 cells after 72 hrs by MTT assay Antiproliferative activity against apoptosis resistant human U373 cells after 72 hrs by MTT assay	[PMID: 26883150]
U-87MG ATCC	IC₅₀	12.5 μM Compound: 1	Cytotoxicity against human U87 cells assessed as cell growth inhibition measured after 24 hrs by MTT assay Cytotoxicity against human U87 cells assessed as cell growth inhibition measured after 24 hrs by MTT assay	[PMID: 34161865]

Chemical Information

CAS No. 906748-38-7
Appearance Solid
Molecular Weight 262.26
Formula C₁₆H₁₀N₂O₂
Color Dark purple to black
SMILES

O=C1/C(C2=CC=CC=C2N1)=C3NC4=CC=CC=C4C\3=O
Synonyms

(Z)-Couroupitine B; (Z)-Indigo red; (Z)-Indigopurpurin
Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
In solvent	-80°C	6 months
	-20°C	1 month

Solvent & Solubility

In Vitro:

DMSO : 4.17 mg/mL (15.90 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

3.8130 mL

19.0650 mL

38.1301 mL

5 mM

0.7626 mL

3.8130 mL

7.6260 mL

10 mM

0.3813 mL

1.9065 mL

3.8130 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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Dilution Calculator

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Purity & Documentation

Select Batch:

Purity: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (274 KB)
SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

References

[1]. Nguyen D T, et al. Synthesis of new indirubin derivatives and their in vitro anticancer activity[J]. Chemical Papers, 2019, 73: 1083-1092.

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
DMSO	1 mM	3.8130 mL	19.0650 mL	38.1301 mL	95.3252 mL
	5 mM	0.7626 mL	3.8130 mL	7.6260 mL	19.0650 mL
	10 mM	0.3813 mL	1.9065 mL	3.8130 mL	9.5325 mL
	15 mM	0.2542 mL	1.2710 mL	2.5420 mL	6.3550 mL

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(Z)-Indirubin

Bioactive Compound Library Max

Bioactive Compound Library

Apoptosis Compound Library

Anti-Cancer Compound Library

Traditional Chinese Medicine Active Compound Library

Anti-Blood Cancer Compound Library

Cell Death Library

Anti-Hematopathy Compound Library

MCE Bioactive Compound Library

Non-Alcoholic Fatty Liver Disease (NAFLD) Compound Library

High-Throughput Bioactive Compound Library

Mongolian Medicine Compound Library

Biological Activity

Chemical Information

Solvent & Solubility

Purity & Documentation

References

Help & FAQs

Complete Stock Solution Preparation Table

(Z)-Indirubin

Bioactive Compound Library Max

Bioactive Compound Library

Apoptosis Compound Library

Anti-Cancer Compound Library

Traditional Chinese Medicine Active Compound Library

Anti-Blood Cancer Compound Library

Cell Death Library

Anti-Hematopathy Compound Library

MCE Bioactive Compound Library

Non-Alcoholic Fatty Liver Disease (NAFLD) Compound Library

High-Throughput Bioactive Compound Library

Mongolian Medicine Compound Library

Biological Activity

Chemical Information

Solvent & Solubility

Purity & Documentation

References

Help & FAQs

Complete Stock Solution Preparation Table

(Z)-Indirubin Related Classifications

HY-N0117A Related Classifications