Sivifene
Based on 1 Customer Validation
Sivifene (A-007) is a triaryl hydrazone. Sivifene has anticancer activity and immunomodulatory effects. Sivifene regulates immune regulation by upregulating CD45 T lymphocyte surface receptors.
For research use only. We do not sell to patients.
- Purity: 99.41%
- CAS No.: 2675-35-6
- Formula: C19H14N4O6
- Molecular Weight:394.34
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Storage:Powder -20°C, 3 years ; In solvent -80°C, 6 months , -20°C, 1 month
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
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Immunology/Inflammation Compound Library
HY-L007
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Anti-Cancer Compound Library
HY-L025
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Clinical Compound Library
HY-L026
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Drug Repurposing Compound Library
HY-L035
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Human Metabolite Library
HY-L123
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Bioactive Compound Library Max
HY-L181
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Drug Repurposing Compound Library Plus
HY-L035P
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Clinical Compound Library Plus
HY-L026P
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Bioactive Compound Library
HY-L001
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High-Throughput Bioactive Compound Library
HY-L205
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Mass Spectrometry Human Metabolite Library
HY-L215
Biological Activity
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Cell Line
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Type | Value | Description | References |
|---|---|---|---|---|
| Breast cancer cell line | IC50 |
>10 μg/mL
Compound: A-007
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Tested for anticancer activity against human breast cancer
Tested for anticancer activity against human breast cancer
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[PMID: 11514168] |
| Colon carcinoma cell | IC50 |
15.2 μg/mL
Compound: 1
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In vitro cytotoxicity in Human colon tumor cells; (>12+/-4, >12+/-5)
In vitro cytotoxicity in Human colon tumor cells; (>12+/-4, >12+/-5)
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[PMID: 14521417] |
| MDA-MB-435 | IC50 |
2.1 μg/mL
Compound: 1
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In vitro cytotoxicity in MDA-MB-435 cell line
In vitro cytotoxicity in MDA-MB-435 cell line
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[PMID: 14521417] |
| Melanoma cell | IC50 |
>10 μg/mL
Compound: A-007
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Tested for anticancer activity against human melanoma
Tested for anticancer activity against human melanoma
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[PMID: 11514168] |
| Melanoma tumor cell line | IC50 |
3.5 μg/mL
Compound: 1
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In vitro cytotoxicity in estrogen receptor and progesterone receptor positive Human breast tumor cells; (2.5+/-0.9, 0.5+/-1)
In vitro cytotoxicity in estrogen receptor and progesterone receptor positive Human breast tumor cells; (2.5+/-0.9, 0.5+/-1)
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[PMID: 14521417] |
| Melanoma tumor cell line | IC50 |
7 μg/mL
Compound: 1
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In vitro cytotoxicity in Human melanoma tumor cells; (5+/-4, >10)
In vitro cytotoxicity in Human melanoma tumor cells; (5+/-4, >10)
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[PMID: 14521417] |
| Ovarian cancer cell line | IC50 |
9 μg/mL
Compound: A-007
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Tested for anticancer activity against human ovarian cancer
Tested for anticancer activity against human ovarian cancer
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[PMID: 11514168] |
| T-cell | IC50 |
3.2 μg/mL
Compound: 1
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Cytotoxicity in HH T-lymphocytic leukemia cells
Cytotoxicity in HH T-lymphocytic leukemia cells
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[PMID: 12419372] |
| NCT Number | Sponsor | Condition | Start Date |
Phase
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|---|---|---|---|---|
| NCT01329991 | Plexxikon| | 2011-05 | PHASE1 |
Chemical Information
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CAS No. 2675-35-6
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Appearance Solid
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Molecular Weight 394.34
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Formula C19H14N4O6
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Color Brown to reddish brown
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SMILES
O=[N+](C1=CC=C(N/N=C(C2=CC=C(O)C=C2)\C3=CC=C(O)C=C3)C([N+]([O-])=O)=C1)[O-]
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Synonyms
A-007
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Powder -20°C 3 years In solvent -80°C 6 months -20°C 1 month
Solvent & Solubility
DMSO : 100 mg/mL (253.59 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (6.34 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (6.34 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
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Data Sheet (272 KB)
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SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Portuguese - PT (251 KB)
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Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 2.5359 mL | 12.6794 mL | 25.3588 mL | 63.3971 mL |
| 5 mM | 0.5072 mL | 2.5359 mL | 5.0718 mL | 12.6794 mL | |
| 10 mM | 0.2536 mL | 1.2679 mL | 2.5359 mL | 6.3397 mL | |
| 15 mM | 0.1691 mL | 0.8453 mL | 1.6906 mL | 4.2265 mL | |
| 20 mM | 0.1268 mL | 0.6340 mL | 1.2679 mL | 3.1699 mL | |
| 25 mM | 0.1014 mL | 0.5072 mL | 1.0144 mL | 2.5359 mL | |
| 30 mM | 0.0845 mL | 0.4226 mL | 0.8453 mL | 2.1132 mL | |
| 40 mM | 0.0634 mL | 0.3170 mL | 0.6340 mL | 1.5849 mL | |
| 50 mM | 0.0507 mL | 0.2536 mL | 0.5072 mL | 1.2679 mL | |
| 60 mM | 0.0423 mL | 0.2113 mL | 0.4226 mL | 1.0566 mL | |
| 80 mM | 0.0317 mL | 0.1585 mL | 0.3170 mL | 0.7925 mL | |
| 100 mM | 0.0254 mL | 0.1268 mL | 0.2536 mL | 0.6340 mL |
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