FPR Agonist 43
Based on 2 publication(s) in Google Scholar
FPR Agonist 43 (compound 43) is a dual formyl peptide receptor 1 (FPR1) and formyl peptide receptor 2 (FPR2)/ALX agonist.
Nos produits utilisent uniquement pour la recherche. Nous ne vendons pas aux patients.
- Pureté : 99.79%
- CAS No.: 903895-98-7
- Formule: C20H21ClN4O2
- Masse moléculaire:384.86
-
Stockage:
4°C, sealed storage, away from moisture
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
-
Activité biologique
-
Chemical Information
-
Solvant et solubilité
- Pureté et documentation
- Références
-
Help & FAQs
Publications Citing Use of MedChemExpress (MCE) FPR Agonist 43
More
Activité biologique
Description
IC50 & Target
FPR1, FPR2/ALX[1]
Cellular Effect
|
Cell Line
|
Type | Value | Description | References |
|---|---|---|---|---|
| CHO | EC50 |
0.044 μM
Compound: 1
|
Agonist activity at human recombinant FPRL1 expressed in CHO cells assessed as calcium flux by aequorin based bioluminescence assay
Agonist activity at human recombinant FPRL1 expressed in CHO cells assessed as calcium flux by aequorin based bioluminescence assay
|
[PMID: 17920884] |
| CHO | EC50 |
0.044 μM
Compound: 43
|
Activity at human FPRL1-mediated calcium mobilization in CHO cells
Activity at human FPRL1-mediated calcium mobilization in CHO cells
|
[PMID: 16697190] |
| PMNL | IC50 |
0.24 μM
Compound: 43
|
Inhibition of IL8-induced PMN migration
Inhibition of IL8-induced PMN migration
|
[PMID: 16697190] |
| PMNL | IC50 |
0.64 μM
Compound: 43
|
Inhibition of fMLP-induced PMN migration
Inhibition of fMLP-induced PMN migration
|
[PMID: 16697190] |
In Vitro
FPR Agonist 43 (10-5-107 nM) is actively potent in the cAMP assay in FPR2/ALX over-expressing CHO cells[1].
FPR Agonist 43 is also active in the GTPγ binding assay (IC50=207±51 nM)[1].
FPR1 is the preferred receptor for FPR Agonist 43 in in both human neutrophils and possibly also in mouse cells[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. .
-
Cell Line:Chinese hamster ovary (CHO) over-expressing human FPR2/ALX receptors
-
Concentration:10-5, 10-3, 0.1, 10, 1000, 105, 107 nM
-
Incubation Time:
-
Result:Actively potent in the cAMP assay in FPR2/ALX over-expressing CHO cells (IC50 =11.6±1.9 nM).
Chemical Information
-
CAS No. 903895-98-7
-
Appearance Solid
-
Masse moléculaire 384.86
-
Formule C20H21ClN4O2
-
Color White to light yellow
-
SMILES
O=C(NC1=C(C(C)C)N(C)N(C2=CC=CC=C2)C1=O)NC3=CC=C(Cl)C=C3
-
Livraison
Room temperature in continental US; may vary elsewhere.
-
Stockage
4°C, sealed storage, away from moisture
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
Publications (2)
-
Journal Impact Factor
-
Most Recent
-
Cancer Res
DLGAP1-AS2-Mediated Phosphatidic Acid Synthesis Activates YAP Signaling and Confers Chemoresistance in Squamous Cell Carcinoma. [Abstract]2022 Aug 16;82(16):2887-2903. PMID: 35731019 -
Nat Commun
Molecular recognition of formylpeptides and diverse agonists by the formylpeptide receptors FPR1 and FPR2. [Abstract]2022 Feb 25;13(1):1054. PMID: 35217703
Solvant et solubilité
In Vitro:
DMSO : 37.5 mg/mL (97.44 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
H2O : < 0.1 mg/mL (insoluble)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
In Vivo:
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:
-
-
-
-
Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
-
%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
-
%+
-
+%Tween-80 + +
-
%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL. * In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Pureté et documentation
-
Fiche technique (277 KB)
-
SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
-
Instruction de manipulation (2659 KB)
Références
[1]. Planagumà A, et al. Lack of activity of 15-epi-lipoxin A₄ on FPR2/ALX and CysLT1 receptors in interleukin-8-driven human neutrophil function. Clin Exp Immunol. 2013 Aug;173(2):298-309. [Content Brief]
[2]. Forsman H, et al. What formyl peptide receptors, if any, are triggered by compound 43 and lipoxin A4? Scand J Immunol. 2011 Sep;74(3):227-234. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 2.5983 mL | 12.9917 mL | 25.9835 mL | 64.9587 mL |
| 5 mM | 0.5197 mL | 2.5983 mL | 5.1967 mL | 12.9917 mL | |
| 10 mM | 0.2598 mL | 1.2992 mL | 2.5983 mL | 6.4959 mL | |
| 15 mM | 0.1732 mL | 0.8661 mL | 1.7322 mL | 4.3306 mL | |
| 20 mM | 0.1299 mL | 0.6496 mL | 1.2992 mL | 3.2479 mL | |
| 25 mM | 0.1039 mL | 0.5197 mL | 1.0393 mL | 2.5983 mL | |
| 30 mM | 0.0866 mL | 0.4331 mL | 0.8661 mL | 2.1653 mL | |
| 40 mM | 0.0650 mL | 0.3248 mL | 0.6496 mL | 1.6240 mL | |
| 50 mM | 0.0520 mL | 0.2598 mL | 0.5197 mL | 1.2992 mL | |
| 60 mM | 0.0433 mL | 0.2165 mL | 0.4331 mL | 1.0826 mL | |
| 80 mM | 0.0325 mL | 0.1624 mL | 0.3248 mL | 0.8120 mL |
FPR Agonist 43 Related Classifications
HY-19574 Related Classifications
Powered by Bioz