TCH-165
Based on 2 publication(s) in Google Scholar
TCH-165 is a small molecule modulator of proteasome assembly, which increases 20S levels and facilitates 20S-mediated protein degradation.
For research use only. We do not sell to patients.
- Purity: 98.42%
- CAS No.: 1446350-60-2
- Formula: C39H37N3O3
- Molecular Weight:595.73
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Storage:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 2 years , -20°C, 1 year
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
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Ubiquitination Compound Library
HY-L050
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Publications Citing Use of MedChemExpress (MCE) TCH-165
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Biological Activity
Proteasome assembly[1]
TCH-165 (0.01-10 μM; 72 hours; RPMI8226 and U87MG cells) treatment inhibits cell growth of RPMI8226 and U87MG cells with IC50 of 1.6 μM and 2.4 μM, respectively[1].
TCH-165 (0-10 μM; 24 hours; HEK293T cells) treatment enhances ODC degradation is blocked by BTZ indicated that this event is proteasome-mediated. TCH-165 enhances proteolytic degradation in a concentration-dependent manner[1].
TCH-165 enhances the chymotrypsin-like (CT-L), trypsin-like (Tryp-L) and caspase-like (Casp-L) activities with EC50s of 4.2 μM, 3.2 μM and 4.7 μM, respectively[1].
TCH-165 enhances 20S-mediated degradation of IDPs, α-syn, and tau in vitro, and does not induce the degradation of structured proteins such as GAPDH[1].
TCH-165-treated cells display a decrease in the assembled 26S and an increase in the 20S proteasome. TCH-165 regulates the dynamic equilibrium between the 20S and 26S proteasome complexes, favoring 20S-mediated protein degradation[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:RPMI8226 and U87MG cells
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Concentration:0.01-10 μM
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Incubation Time:72 hours
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Result:Inhibited cell growth of RPMI8226 and U87MG cells with IC50 of 1.6 μM and 2.4 μM, respectively.
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Cell Line:HEK293T cells
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Concentration:0 μM, 3 μM, 10 μM
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Incubation Time:24 hours
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Result:Enhanced proteolytic degradation in a concentration-dependent manner.
Chemical Information
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CAS No. 1446350-60-2
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Appearance Solid
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Molecular Weight 595.73
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Formula C39H37N3O3
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Color Light yellow to yellow
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SMILES
O=C([C@]1(C2=CC=CC=C2)N=C(C3=CC=C(OC)C=C3)N(CC4=CC=CC=C4)[C@@H]1C5=CC=C(NCC6=CC=CC=C6)C=C5)OCC
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Powder -20°C 3 years 4°C 2 years In solvent -80°C 2 years -20°C 1 year
Publications (2)
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Journal Impact Factor
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Most Recent
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Eur J Pharmacol
TCH-165 attenuates cardiac ischaemia/reperfusion injury by balancing mitochondrial dynamics via increasing proteasome activity. [Abstract]2023 Oct 15:957:176011. PMID: 37633323 -
Vet Microbiol
PRRSV Nsp12 targets PSMA2 to suppress host proteasome activity and promote viral survival. [Abstract]2026 Feb:313:110835. PMID: 41475191
Solvent & Solubility
DMSO : 250 mg/mL (419.65 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 6.25 mg/mL (10.49 mM); Clear solution
This protocol yields a clear solution of ≥ 6.25 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (62.5 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
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Data Sheet (284 KB)
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SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
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Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 1.6786 mL | 8.3931 mL | 16.7861 mL | 41.9653 mL |
| 5 mM | 0.3357 mL | 1.6786 mL | 3.3572 mL | 8.3931 mL | |
| 10 mM | 0.1679 mL | 0.8393 mL | 1.6786 mL | 4.1965 mL | |
| 15 mM | 0.1119 mL | 0.5595 mL | 1.1191 mL | 2.7977 mL | |
| 20 mM | 0.0839 mL | 0.4197 mL | 0.8393 mL | 2.0983 mL | |
| 25 mM | 0.0671 mL | 0.3357 mL | 0.6714 mL | 1.6786 mL | |
| 30 mM | 0.0560 mL | 0.2798 mL | 0.5595 mL | 1.3988 mL | |
| 40 mM | 0.0420 mL | 0.2098 mL | 0.4197 mL | 1.0491 mL | |
| 50 mM | 0.0336 mL | 0.1679 mL | 0.3357 mL | 0.8393 mL | |
| 60 mM | 0.0280 mL | 0.1399 mL | 0.2798 mL | 0.6994 mL | |
| 80 mM | 0.0210 mL | 0.1049 mL | 0.2098 mL | 0.5246 mL | |
| 100 mM | 0.0168 mL | 0.0839 mL | 0.1679 mL | 0.4197 mL |
TCH-165 Related Classifications
HY-120722 Related Classifications
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