Tenuifoliside C
Based on 1 Customer Validation
Tenuifoliside C, isolated from polygala tenuifolia willd, significantly inhibits chlorzoxazone 6-hydroxylation catalyzed by CYP2E1.
For research use only. We do not sell to patients.
- Purity: 99.57%
- CAS No.: 139726-37-7
- Formula: C35H44O19
- Molecular Weight:768.71
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Storage:
4°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
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Biological Activity
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Cell Line
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Type | Value | Description | References |
|---|---|---|---|---|
| A549 | IC50 |
26 μM
Compound: 5
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Cytotoxicity against human A549 cells assessed as cell growth inhibition after 48 hrs by SRB assay
Cytotoxicity against human A549 cells assessed as cell growth inhibition after 48 hrs by SRB assay
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[PMID: 25466198] |
| BV-2 | IC50 |
26.96 μM
Compound: 5
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Inhibition of LPS-induced nitric oxide production in mouse BV2 cells treated for 30 mins prior to LPS stimulation for 24 hrs by Griess assay
Inhibition of LPS-induced nitric oxide production in mouse BV2 cells treated for 30 mins prior to LPS stimulation for 24 hrs by Griess assay
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[PMID: 25466198] |
| HCT-15 | IC50 |
18.3 μM
Compound: 5
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Cytotoxicity against human HCT15 cells assessed as cell growth inhibition after 48 hrs by SRB assay
Cytotoxicity against human HCT15 cells assessed as cell growth inhibition after 48 hrs by SRB assay
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[PMID: 25466198] |
| SK-MEL-2 | IC50 |
21.55 μM
Compound: 5
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Cytotoxicity against human SK-MEL-2 cells assessed as cell growth inhibition after 48 hrs by SRB assay
Cytotoxicity against human SK-MEL-2 cells assessed as cell growth inhibition after 48 hrs by SRB assay
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[PMID: 25466198] |
| SK-OV-3 | IC50 |
24.64 μM
Compound: 5
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Cytotoxicity against human SKOV3 cells assessed as cell growth inhibition after 48 hrs by SRB assay
Cytotoxicity against human SKOV3 cells assessed as cell growth inhibition after 48 hrs by SRB assay
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[PMID: 25466198] |
Chemical Information
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CAS No. 139726-37-7
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Appearance Solid
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Molecular Weight 768.71
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Formula C35H44O19
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Color Light yellow to yellow
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SMILES
OC[C@@]1([C@H]([C@H](O)[C@@H](CO)O1)OC(/C=C/C2=CC(OC)=C(OC)C(OC)=C2)=O)O[C@H]3O[C@@H]([C@@H](O)[C@H](O)[C@H]3O)COC(/C=C/C4=CC(OC)=C(O)C(OC)=C4)=O
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Structure Classification
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Initial Source
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
4°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
Solvent & Solubility
DMSO : 100 mg/mL (130.09 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (3.25 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (3.25 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL. * In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
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Data Sheet (275 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Portuguese - PT (252 KB)
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Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 1.3009 mL | 6.5044 mL | 13.0088 mL | 32.5220 mL |
| 5 mM | 0.2602 mL | 1.3009 mL | 2.6018 mL | 6.5044 mL | |
| 10 mM | 0.1301 mL | 0.6504 mL | 1.3009 mL | 3.2522 mL | |
| 15 mM | 0.0867 mL | 0.4336 mL | 0.8673 mL | 2.1681 mL | |
| 20 mM | 0.0650 mL | 0.3252 mL | 0.6504 mL | 1.6261 mL | |
| 25 mM | 0.0520 mL | 0.2602 mL | 0.5204 mL | 1.3009 mL | |
| 30 mM | 0.0434 mL | 0.2168 mL | 0.4336 mL | 1.0841 mL | |
| 40 mM | 0.0325 mL | 0.1626 mL | 0.3252 mL | 0.8131 mL | |
| 50 mM | 0.0260 mL | 0.1301 mL | 0.2602 mL | 0.6504 mL | |
| 60 mM | 0.0217 mL | 0.1084 mL | 0.2168 mL | 0.5420 mL | |
| 80 mM | 0.0163 mL | 0.0813 mL | 0.1626 mL | 0.4065 mL | |
| 100 mM | 0.0130 mL | 0.0650 mL | 0.1301 mL | 0.3252 mL |
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