Testosterone sulfate (pyridinium)
Based on 1 publication(s) in Google Scholar
Testosterone sulfate pyridinium is a Testosterone sulfate with a pyridinium. Testosterone sulfate pyridinium is the metabolite of Testosterone. Testosterone is the main male sex hormone which determination is useful for assessment of androgen status.
For research use only. We do not sell to patients.
- Purity: 99.70%
- CAS No.: 34991-10-1
- Formula: C24H33NO5S
- Molecular Weight:447.59
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Storage:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
Publications Citing Use of MedChemExpress (MCE) Testosterone sulfate (pyridinium)
MoreAll Endogenous Metabolite Isoforms
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Biological Activity
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Human Endogenous Metabolite |
Testosterone sulfate pyridinium is a biomarker for exogenous testosterone intake, however, it needs to undergo hydrolysis (decoupling) to be converted into free testosterone before it can be effectively detected by analytical instruments such as chromatography-mass spectrometry. Enzymatic hydrolysis using an enzyme preparation containing sulfatase is an effective and reliable method for releasing free testosterone for detection[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 34991-10-1
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Appearance Solid
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Molecular Weight 447.59
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Formula C24H33NO5S
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Color White to off-white
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SMILES
C[C@@]12[C@]3([H])[C@](CCC1=CC(CC2)=O)([H])[C@@]4([H])[C@](CC3)([C@H](CC4)OS(=O)(O)=O)C.C5=CC=NC=C5
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Structure Classification
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Initial Source
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Publications (1)
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Journal Impact Factor
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Most Recent
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Am J Pathol
Testosterone-Induced H3K27 Deacetylation Participates in Granulosa Cell Proliferation Suppression and Pathogenesis of Polycystic Ovary Syndrome. [Abstract]2024 Dec;194(12):2326-2340. PMID: 39243944
Solvent & Solubility
DMSO : 100 mg/mL (223.42 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (5.59 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (5.59 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
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Data Sheet (275 KB)
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SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
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Handling Instructions (2659 KB)
References
[1]. Pizzato EC, Filonzi M, Rosa HSD, de Bairros AV. Pretreatment of different biological matrices for exogenous testosterone analysis: a review. Toxicol Mech Methods. 2017;27(9):641-656. [Content Brief]
[2]. Mroczko B, et al. Niektóre problemy zwiazane z oznaczaniem testosteronu [Some problems with testosterone determination]. Endokrynol Pol. 2007;58(5):440-445. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
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| DMSO | 1 mM | 2.2342 mL | 11.1709 mL | 22.3419 mL | 55.8547 mL |
| 5 mM | 0.4468 mL | 2.2342 mL | 4.4684 mL | 11.1709 mL | |
| 10 mM | 0.2234 mL | 1.1171 mL | 2.2342 mL | 5.5855 mL | |
| 15 mM | 0.1489 mL | 0.7447 mL | 1.4895 mL | 3.7236 mL | |
| 20 mM | 0.1117 mL | 0.5585 mL | 1.1171 mL | 2.7927 mL | |
| 25 mM | 0.0894 mL | 0.4468 mL | 0.8937 mL | 2.2342 mL | |
| 30 mM | 0.0745 mL | 0.3724 mL | 0.7447 mL | 1.8618 mL | |
| 40 mM | 0.0559 mL | 0.2793 mL | 0.5585 mL | 1.3964 mL | |
| 50 mM | 0.0447 mL | 0.2234 mL | 0.4468 mL | 1.1171 mL | |
| 60 mM | 0.0372 mL | 0.1862 mL | 0.3724 mL | 0.9309 mL | |
| 80 mM | 0.0279 mL | 0.1396 mL | 0.2793 mL | 0.6982 mL | |
| 100 mM | 0.0223 mL | 0.1117 mL | 0.2234 mL | 0.5585 mL |
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