Tetraethylammonium chloride

Name: Tetraethylammonium chloride
Brand: MedChemExpress
SKU: HY-B1793
Rating: 4.5 (3 reviews)

Cat. No.: HY-B1793 Purity: ≥98.0%: FAQs
Data Sheet SDS COA Handling Instructions

Molarity Calculator

Tetraethylammonium chloride is a non-selective potassium channel blocker. Tetraethylammonium chloride is a good substrate for organic cation transporter (OCTN1). Tetraethylammonium chloride antitumor properties.

For research use only. We do not sell to patients.

Tetraethylammonium chloride Chemical Structure

CAS No. : 56-34-8

Size	Price	Stock	Quantity

Free Sample (0.1 - 0.5 mg)		Apply Now
Solid + Solvent
10 mM * 1 mL in DMSO ready for reconstitution	USD 28	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	USD 28	In-stock	Estimated Time of Arrival: December 31
Solid
500 mg	USD 25	In-stock	Estimated Time of Arrival: December 31
1 g	USD 40	In-stock	Estimated Time of Arrival: December 31
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Customer Review

Based on 3 publication(s) in Google Scholar

3 Publications Citing Use of MCE Tetraethylammonium chloride

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Biological Activity
Purity & Documentation
References
Customer Review

Description

In Vitro

Tetraethylammonium (0.2-60 mM; 24-72 hours; C6 and 9L glioma cells) treatment inhibits the proliferation of C6 and 9L cells in a dose- and time-dependent manner^[1].
Tetraethylammonium (40 mM; 24-72 hours; C6 and 9L glioma cells) treatment significantly increases apoptosis in cells^[1].
Tetraethylammonium (40 mM; 12-48 hours; C6 and 9L glioma cells) treatment significantly elevates Bax/Bcl-2 protein ratio in a time-dependent manner^[1].
The generation of intracellular ROS increased in C6 and 9L cells by the addition of 20 and 40 mM Tetraethylammonium^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Proliferation Assay^[1]

Cell Line:	Rat C6 and 9L glioma cells
Concentration:	0.2 mM, 2 mM, 20 mM, 40 mM and 60 mM
Incubation Time:	24 hours, 48 hours and 72 hours
Result:	Inhibited the proliferation of C6 and 9L cells in a dose- and time-dependent manner.

Apoptosis Analysis^[1]

Cell Line:	Rat C6 and 9L glioma cells
Concentration:	40 mM
Incubation Time:	24 hours, 48 hours and 72 hours
Result:	Significantly increased apoptosis in cells.

Western Blot Analysis^[1]

Cell Line:	Rat C6 and 9L glioma cells
Concentration:	40 mM
Incubation Time:	12 hours, 24 hours, 48 hours
Result:	The expression of Bax was markedly increased, while that of Bcl-2 showed a decreasing trend 12, 24 and 48 h.

In Vivo

Tetraethylammonium (1 mM, 3 mM, and 5 mM) significantly increases the amplitude and frequency of contractility of colon and rectum from rats in longitudinal and circular direction. Tetraethylammonium at 5 mM and 15 mM concentrations shows no effect on histology of colon and rectum from rats that are administered locally with Tetraethylammonium into colon lumen from anus for 10 days^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

165.70

Formula

C₈H₂₀ClN

CAS No.

56-34-8

Appearance

Solid

Color

White to off-white

SMILES

CC[N+](CC)(CC)CC.[Cl-]

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, sealed storage, away from moisture

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

Solvent & Solubility

In Vitro:

H₂O : ≥ 100 mg/mL (603.50 mM)

DMSO : 100 mg/mL (603.50 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

*"≥" means soluble, but saturation unknown.

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	6.0350 mL	30.1750 mL	60.3500 mL
5 mM	1.2070 mL	6.0350 mL	12.0700 mL
10 mM	0.6035 mL	3.0175 mL	6.0350 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (15.09 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (15.09 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.5 mg/mL (15.09 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: PBS
Solubility: 100 mg/mL (603.50 mM); Clear solution; Need ultrasonic

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).

Purity & Documentation

Purity: ≥98.0%

Select Batch:

Data Sheet (276 KB) SDS (393 KB)

COA (247 KB) HNMR (234 KB)

Handling Instructions (2659 KB)

References

[1]. K B Yang, et al. Tetraethylammonium inhibits glioma cells via increasing production of intracellular reactive oxygen species. Chemotherapy. 2009;55(5):372-80. [Content Brief]

[2]. Zhe Li, et al. Tetraethylammonium enhances the rectal and colonic motility in rats and human in vitro. Naunyn Schmiedebergs Arch Pharmacol. 2011 Aug;384(2):147-55. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

H₂O / DMSO	1 mM	6.0350 mL	30.1750 mL	60.3500 mL	150.8751 mL
	5 mM	1.2070 mL	6.0350 mL	12.0700 mL	30.1750 mL
	10 mM	0.6035 mL	3.0175 mL	6.0350 mL	15.0875 mL
	15 mM	0.4023 mL	2.0117 mL	4.0233 mL	10.0583 mL
	20 mM	0.3018 mL	1.5088 mL	3.0175 mL	7.5438 mL
	25 mM	0.2414 mL	1.2070 mL	2.4140 mL	6.0350 mL
	30 mM	0.2012 mL	1.0058 mL	2.0117 mL	5.0292 mL
	40 mM	0.1509 mL	0.7544 mL	1.5088 mL	3.7719 mL
	50 mM	0.1207 mL	0.6035 mL	1.2070 mL	3.0175 mL
	60 mM	0.1006 mL	0.5029 mL	1.0058 mL	2.5146 mL
	80 mM	0.0754 mL	0.3772 mL	0.7544 mL	1.8859 mL
	100 mM	0.0604 mL	0.3018 mL	0.6035 mL	1.5088 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.