Tetrahydropalmatine hydrochloride
Based on 3 publication(s) in Google Scholar
Tetrahydropalmatine (DL-Tetrahydropalmatine) hydrochloride possesses analgesic effects. Tetrahydropalmatine hydrochloride acts through inhibition of amygdaloid release of dopamine to inhibit an epileptic attack in rats.
For research use only. We do not sell to patients.
- Purity: 99.92%
- CAS No.: 6024-85-7
- Formula: C21H26ClNO4
- Molecular Weight:391.89
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Storage:
4°C, sealed storage, away from moisture and light
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Protocol
- Purity & Documentation
- References
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Help & FAQs
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Publications Citing Use of MedChemExpress (MCE) Tetrahydropalmatine hydrochloride
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Customer Validation & Images
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In Vivo Imaging
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IHC
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Bio/Physico-chemical Assay
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Cell Imaging/Staining
All Dopamine Receptor Isoforms
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Biological Activity
Dopamine[1]
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 6024-85-7
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Appearance Solid
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Molecular Weight 391.89
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Formula C21H26ClNO4
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Color White to off-white
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SMILES
COC1=CC=C2C(CN3CCC4=CC(OC)=C(OC)C=C4C3C2)=C1OC.[H]Cl
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Synonyms
DL-Tetrahydropalmatine hydrochloride
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Structure Classification
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Initial Source
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
4°C, sealed storage, away from moisture and light
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)
Publications (3)
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Journal Impact Factor
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Most Recent
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Biomed Pharmacother
Tetrahydropalmatine: Orchestrating survival - Regulating autophagy and apoptosis via the PI3K/AKT/mTOR pathway in perforator flaps. [Abstract]2023 Dec 31:169:115887. PMID: 37984303
Tetrahydropalmatine hydrochloride purchased from MedChemExpress. Usage Cited in: Biomed Pharmacother. 2023 Dec 31:169:115887. [Abstract]
High-definition digital photos and Doppler images illustrating flap survival and necrosis areas on days 0 and 7 for both the control and Tetrahydropalmatine (THP, 2 mg/kg) groups.
Tetrahydropalmatine hydrochloride purchased from MedChemExpress. Usage Cited in: Biomed Pharmacother. 2023 Dec 31:169:115887. [Abstract]
IHC for CD34-positive microvessels in the choke II region of the control and Tetrahydropalmatine (THP) groups (200X); scale bar, 100 µm.
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Chem Biol Drug Des
Tetrahydropalmatine Alleviates Osteoarthritis-Associated Pain and Inflammation by Suppressing KDM4A/MDM2/HIF-1α-Mediated M1 Macrophage Polarization. [Abstract]2026 Mar;107(3):e70279. PMID: 41857767 -
J Anim Sci
2022 Apr 1;100(4):skac069. PMID: 35247050
Tetrahydropalmatine hydrochloride purchased from MedChemExpress. Usage Cited in: J Anim Sci. 2022 Apr 1;100(4):skac069. [Abstract]
The accumulation of spermine or spermidine in boar sperm was inhibited by 60 μmol/L quinidine or 60 μmol/L Tetrahydropalmatine (THP), but neither 60 μmol/L mildronate nor 60 μmol/L l-carnitine.
Tetrahydropalmatine hydrochloride purchased from MedChemExpress. Usage Cited in: J Anim Sci. 2022 Apr 1;100(4):skac069. [Abstract]
SM-FITC or SD-FITC in the sperm was significantly decreased after the supplementation of 60 μmol/L quinidine or 60 μmol/L Tetrahydropalmatine (THP).
Solvent & Solubility
DMSO : 25 mg/mL (63.79 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
H2O : 4 mg/mL (10.21 mM; ultrasonic and warming and heat to 60°C)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: 2.5 mg/mL (6.38 mM); Clear solution; Need ultrasonic
This protocol yields a clear solution of 2.5 mg/mL.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (6.38 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL. * In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Protocol
Rats[1]
Male Sprague-Dawley rats, weighing 250-320 g, are used in the present study. The animals are housed in a temperature-regulated (22±1°C) room on 12:12 h light/dark cycles with food and water available ad libitum. The light is turned on at 06:00 h and turned off at 18:00 h. At least two major groups of animals are studied. (1) Vehicle-treated rats: received an i.p. injection of 0.9% saline plus Picrotoxin (3-4 mg/kg, s.c.). (2) Tetrahydropalmatine-treated rats: receive an injection of Tetrahydropalmatine (10 mg/kg, i.p.) plus Picrotoxin (3-4 mg/kg, s.c.). The effects of s.c. administration of Picrotoxin or Tetrahydropalmatine on locomotor activity are assessed in unanesthetized rats. On the other hand, the effects of Picrotoxin or Tetrahydropalmatine on amygdaloid DA release are assessed in rats under urethane (1.4 g/kg, i.p.) anesthesia[1].
Seventy-two unanesthetized rats are randomly divided into nine groups, each of eight rats. The animals are adapted to the behavior apparatus for 30 min before an injection of Picrotoxin (3 or 4 mg/kg, s.c.), Tetrahydropalmatine (10 or 15 mg/kg, i.p.) or saline. Then, the locomotor activities of these rats are recorded during the 30-min period following the injections[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Purity & Documentation
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Data Sheet (276 KB)
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SDS (394 KB)
- English - EN (394 KB)
- Français - FR (394 KB)
- Deutsch - DE (394 KB)
- Norwegian - NO (394 KB)
- Español - ES (394 KB)
- Swedish - SV (394 KB)
- Italian - IT (394 KB)
- Portuguese - PT (394 KB)
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Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| H2O / DMSO | 1 mM | 2.5517 mL | 12.7587 mL | 25.5174 mL | 63.7934 mL |
| 5 mM | 0.5103 mL | 2.5517 mL | 5.1035 mL | 12.7587 mL | |
| 10 mM | 0.2552 mL | 1.2759 mL | 2.5517 mL | 6.3793 mL | |
| DMSO | 15 mM | 0.1701 mL | 0.8506 mL | 1.7012 mL | 4.2529 mL |
| 20 mM | 0.1276 mL | 0.6379 mL | 1.2759 mL | 3.1897 mL | |
| 25 mM | 0.1021 mL | 0.5103 mL | 1.0207 mL | 2.5517 mL | |
| 30 mM | 0.0851 mL | 0.4253 mL | 0.8506 mL | 2.1264 mL | |
| 40 mM | 0.0638 mL | 0.3190 mL | 0.6379 mL | 1.5948 mL | |
| 50 mM | 0.0510 mL | 0.2552 mL | 0.5103 mL | 1.2759 mL | |
| 60 mM | 0.0425 mL | 0.2126 mL | 0.4253 mL | 1.0632 mL |
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
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