2. Autophagy
  3. Autophagy
  4. Trimetazidine

Trimetazidine is a selective long chain 3-ketoyl coenzyme A thiolase inhibitor with an IC50 of 75 nM, which can inhibit β-oxidation of free fatty acid (FFA). Trimetazidine is an effective antianginal agent and a cytoprotective agent, has anti-oxidant, anti-inflammatory, antinociceptive and gastroprotective properties. Trimetazidine triggers autophagy. Trimetazidine is also a 3-hydroxyacyl-CoA dehydrogenase (HADHA) inhibitor.

For research use only. We do not sell to patients.

CAS No. : 5011-34-7

Size Price Stock Quantity
Oil + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
ready for reconstitution
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10 mM * 1 mL in DMSO In-stock
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Based on 17 publication(s) in Google Scholar

Other Forms of Trimetazidine:

Trimetazidine Related Antibodies

Top Publications Citing Use of Products

    Trimetazidine purchased from MedChemExpress. Usage Cited in: Adv Sci (Weinh). 2026 Jan 21:e17054.  [Abstract]

    Colony formation assays evaluated the proliferative capacity of MASH‐HCC cells (PA/OA and IL-6/TNF-α/LPS-treated HuH‐7 cells) and non-MASH-HCC cells transfected with vector or OCTN2, with or without Trimetazidine (TMZ) (1 mM).

    Trimetazidine purchased from MedChemExpress. Usage Cited in: Adv Sci (Weinh). 2026 Jan 21:e17054.  [Abstract]

    The FAO inhibitor Trimetazidine (TMZ) (1 mM) abrogated the enhanced migration and invasion of non-MASH-HCC cells caused by OCTN2 overexpression.

    Trimetazidine purchased from MedChemExpress. Usage Cited in: Adv Sci (Weinh). 2026 Jan 21:e17054.  [Abstract]

    Wound healing assays evaluated the migration ability of MASH-HCC and non-MASH-HCC cells transfected with vector or OCTN2, with or without Trimetazidine (TMZ) (1 mM) treatment.

    Trimetazidine purchased from MedChemExpress. Usage Cited in: J Adv Res. 2025 Mar 17:S2090-1232(25)00186-9.  [Abstract]

    OVCAR8-Persister and A2780-Persister cells were pretreated with vehicle or Trimetazidine (TMZ) (100 μM; 24 h) and subsequently treated with various concentrations of paclitaxel (1-1×104 nM; 48 h), after which cell viability was assessed using a CCK-8 assay.

    Trimetazidine purchased from MedChemExpress. Usage Cited in: J Adv Res. 2025 Mar 17:S2090-1232(25)00186-9.  [Abstract]

    Trimetazidine (TMZ) (100 μM; 24 h) pretreatment increased the sensitivity of OVCAR8-Persister and A2780-Persister cells to paclitaxel.

    • Biological Activity

    • Purity & Documentation

    • References

    • Customer Review

    Description

    Trimetazidine is a selective long chain 3-ketoyl coenzyme A thiolase inhibitor with an IC50 of 75 nM, which can inhibit β-oxidation of free fatty acid (FFA). Trimetazidine is an effective antianginal agent and a cytoprotective agent, has anti-oxidant, anti-inflammatory, antinociceptive and gastroprotective properties. Trimetazidine triggers autophagy. Trimetazidine is also a 3-hydroxyacyl-CoA dehydrogenase (HADHA) inhibitor[1][2][3][4].

    IC50 & Target

    IC50: 75 nM (long chain 3-ketoyl coenzyme A thiolase)[2]
    β-oxidation[2]
    Autophagy[3]
    3-hydroxyacyl-CoA dehydrogenase (HADHA)[4]
    Cellular Effect
    Cell Line Type Value Description References
    KB IC50
    47.1 μM
    Compound: 43
    Cytotoxicity in human KB cells
    Cytotoxicity in human KB cells
    		[PMID: 21504156]
    In Vitro

    Trimetazidine (1-100 μM; 24 hours; HUVECs) could enhance the viability of the injured HUVECs induced by oxidation in a certain dose-dependent manner[1].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    Trimetazidine Related Antibodies

    Cell Viability Assay[1]

    Cell Line: Human umbilical vein endothelial cells (HUVECs)
    Concentration: 1 μM,10 μM,100 μM
    Incubation Time: 24 hours
    Result: Enhanced the viability of the injured HUVECs induced by oxidation.
    In Vivo

    Trimetazidine (5-20 mg/kg; oral administration; 1 hour; Swiss albino male mice) in 10 and 20mg/kg doses significantly raises the seizure-threshold current in the ICES test in the mice[5].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    Animal Model: Swiss albino male mice (24-35 g)[4]
    Dosage: 5 mg/kg, 10 mg/kg and 20 mg/kg;
    10 mL/kg body weight
    Administration: Oral administration ; 1 hour
    Result: In 10 and 20mg/kg doses significantly raised the seizure-threshold current in the ICES test.
    Molecular Weight

    266.34

    Formula

    C14H22N2O3
    CAS No.
    Appearance

    Oil

    Color

    White to off-white

    SMILES

    COC1=CC=C(CN2CCNCC2)C(OC)=C1OC
    Shipping

    Room temperature in continental US; may vary elsewhere.
    Storage

    4°C, protect from light, stored under nitrogen

    *In solvent : -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen)

    Solvent & Solubility
    In Vitro: 

    DMSO : 116.67 mg/mL (438.05 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 3.7546 mL 18.7730 mL 37.5460 mL
    5 mM 0.7509 mL 3.7546 mL 7.5092 mL
    View the Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    • Molarity Calculator

    • Dilution Calculator

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

    Mass
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    Volume
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    Molecular Weight *

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    This equation is commonly abbreviated as: C1V1 = C2V2

    Concentration (start)

    C1

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    Volume (start)

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    C2

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    V2

    In Vivo:

    Select the appropriate dissolution method based on your experimental animal and administration route.

    For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
    To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
    The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

    • Protocol 1

      Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

      Solubility: ≥ 2.08 mg/mL (7.81 mM); Clear solution

      This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).

      Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

      Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
    • Protocol 2

      Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

      Solubility: ≥ 2.08 mg/mL (7.81 mM); Clear solution

      This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).

      Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

      Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
    In Vivo Dissolution Calculator
    Please enter the basic information of animal experiments:

    Dosage

    mg/kg

    Animal weight
    (per animal)

    g

    Dosing volume
    (per animal)

    μL

    Number of animals

    Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
    Please enter your animal formula composition:
    %
    DMSO +
    % 
    +
    %
    Tween-80 +
    %
    Saline
    Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
    The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
    Calculation results:
    Working solution concentration: mg/mL
    Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).

    *In solvent : -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen)

    The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
    Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
     If the continuous dosing period exceeds half a month, please choose this protocol carefully.
    Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
    Purity & Documentation

    Purity: 99.84%

    SDS (477 KB)

    COA (247 KB) HNMR (238 KB) RP-HPLC (245 KB) LCMS (87 KB)

    Handling Instructions (2659 KB)
    References

    Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
    DMSO 1 mM 3.7546 mL 18.7730 mL 37.5460 mL 93.8650 mL
    5 mM 0.7509 mL 3.7546 mL 7.5092 mL 18.7730 mL
    10 mM 0.3755 mL 1.8773 mL 3.7546 mL 9.3865 mL
    15 mM 0.2503 mL 1.2515 mL 2.5031 mL 6.2577 mL
    20 mM 0.1877 mL 0.9386 mL 1.8773 mL 4.6932 mL
    25 mM 0.1502 mL 0.7509 mL 1.5018 mL 3.7546 mL
    30 mM 0.1252 mL 0.6258 mL 1.2515 mL 3.1288 mL
    40 mM 0.0939 mL 0.4693 mL 0.9386 mL 2.3466 mL
    50 mM 0.0751 mL 0.3755 mL 0.7509 mL 1.8773 mL
    60 mM 0.0626 mL 0.3129 mL 0.6258 mL 1.5644 mL
    80 mM 0.0469 mL 0.2347 mL 0.4693 mL 1.1733 mL
    100 mM 0.0375 mL 0.1877 mL 0.3755 mL 0.9386 mL
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    Trimetazidine Related Classifications

    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    Trimetazidine5011-34-7AutophagyInhibitorinhibitorinhibit

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