10-Deacetyltaxol
Based on 1 publication(s) in Google Scholar
10-Deacetyltaxol (10-Deacetylpaclitaxel) is a taxane derivative found in Taxus wallichiana Zucc. and exhibits cytotoxicity against human neurotumor cell lines. It shows a concentration-dependent cytotoxic effect on cancer cells and demonstrates activity in the mouse leukemia system. 10-Deacetyltaxol can be used for research on neuroblastoma, glioblastoma multiforme, and lymphocytic leukemia.
For research use only. We do not sell to patients.
- Purity: 99.24%
- CAS No.: 78432-77-6
- Formula: C45H49NO13
- Molecular Weight:811.87
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Storage:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
Publications Citing Use of MedChemExpress (MCE) 10-Deacetyltaxol
MoreAll Endogenous Metabolite Isoforms
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Biological Activity
Description
Cellular Effect
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Cell Line
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Type | Value | Description | References |
|---|---|---|---|---|
| HEK293 | IC50 |
0.076 μM
Compound: 2
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Cytotoxicity against HEK293 cells
Cytotoxicity against HEK293 cells
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[PMID: 17485207] |
| HeLa | IC50 |
0.00075 μM
Compound: 19
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Cytotoxicity against human HeLa cells assessed as reduction in cell viability incubated for 72 hrs by WST-8 dye based assay
Cytotoxicity against human HeLa cells assessed as reduction in cell viability incubated for 72 hrs by WST-8 dye based assay
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[PMID: 28240909] |
| HeLa | IC50 |
0.75 nM
Compound: 19
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Cytotoxicity against human HeLa cells assessed as reduction in cell viability incubated for 72 hrs by WST-8 dye based assay
Cytotoxicity against human HeLa cells assessed as reduction in cell viability incubated for 72 hrs by WST-8 dye based assay
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[PMID: 28240909] |
| KB | IC50 |
0.0015 μg/mL
Compound: 54
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Cytotoxicity against human KB cells
Cytotoxicity against human KB cells
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[PMID: 14987066] |
| KB | ED50 |
0.27 ng/mL
Compound: 5
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Cytotoxicity against human KB cells
Cytotoxicity against human KB cells
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[PMID: 7264680] |
| KB | ED50 |
2.7 x 10-2 μg/mL
Compound: 5
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Cytotoxicity against human KB cells
Cytotoxicity against human KB cells
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[PMID: 7264680] |
In Vitro
10-Deacetyltaxol (10-Deacetylpaclitaxel) (0.1-10.0 μg/mL; 1-24 h) exerts concentration-dependent cytotoxicity in human SK-N-AS and SK-N-FI neuroblastoma cells and human VA-MG-SL and U-373-MG glioblastoma cells, with SK-N-AS being the most sensitive and SK-N-FI the most resistant of the four lines[2].
10-Deacetyltaxol exerts potent cytotoxic effects on KB carcinoma cells, with an ED50 of 2.7×10-2 μg/mL[3].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:Human neuroblastoma cell lines SK-N-AS, SK-N-FI; human glioblastoma multiforme cell lines VA-MG-SL, U-373-MG
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Concentration:0.1 μg/mL (1 h exposure); 1.0 μg/mL (1 h exposure); 10.0 μg/mL (1 h exposure); 0.1 μg/mL (24 h exposure); 1.0 μg/mL (24 h exposure); 10.0 μg/mL (24 h exposure)
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Incubation Time:1 h (exposure); 24 h (exposure)
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Result:Produced 35%, 76%, and 91% toxicity in SK-N-AS cells after 1 hour at 0.1, 1.0, and 10.0 μg/mL, respectively.
Produced 71%, 88%, and 93% toxicity in SK-N-AS cells after 24 hours at 0.1, 1.0, and 10.0 μg/mL, respectively.
Showed 0% toxicity in SK-N-FI cells after 1 hour at 0.1, 1.0, and 10.0 μg/mL.
Yielded 0%, 9%, and 38% toxicity in SK-N-FI cells after 24 hours at 0.1, 1.0, and 10.0 μg/mL, respectively.
Produced 0%, 41%, and 64% toxicity in VA-MG-SL cells after 1 hour at 0.1, 1.0, and 10.0 μg/mL, respectively.
Produced 0%, 22%, and 41% toxicity in VA-MG-SL cells after 24 hours at 0.1, 1.0, and 10.0 μg/mL, respectively.
Yielded 39%, 64%, and 73% toxicity in U-373-MG cells after 1 hour at 0.1, 1.0, and 10.0 μg/mL, respectively.
Yielded 61%, 69%, and 73% toxicity in U-373-MG cells after 24 hours at 0.1, 1.0, and 10.0 μg/mL, respectively.
Was 33-53% less cytotoxic than taxol at equal concentrations and exposure durations across the four cell lines.
In Vivo
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:PS mouse model[3]
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Dosage:16 mg/kg
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Administration:p.o.
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Result:Showed in vivo anti-lymphocytic leukemia activity, producing a 135% T/C at 16 mg/kg in the PS mouse model.
Chemical Information
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CAS No. 78432-77-6
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Appearance Solid
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Molecular Weight 811.87
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Formula C45H49NO13
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Color White to off-white
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SMILES
CC(O[C@]12[C@@]([C@]3([C@@H](O)C[C@@]1([H])OC2)C)([H])[C@@H]([C@]4(C(C)(C([C@@H](O)C3=O)=C(C)[C@@H](OC([C@H](O)[C@H](C5=CC=CC=C5)NC(C6=CC=CC=C6)=O)=O)C4)C)O)OC(C7=CC=CC=C7)=O)=O
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Synonyms
10-Deacetylpaclitaxel
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Structure Classification
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Initial Source
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Publications (1)
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Journal Impact Factor
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Most Recent
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J Sep Sci
Oriented Immobilization Coupled With Ligand Fishing Strategy for Rapid Discovery of Direct HMGB1 Inhibitors in Taxus wallichiana var. mairei. [Abstract]2026 Apr;49(4):e70402. PMID: 41906428
Solvent & Solubility
In Vitro:
DMSO : 100 mg/mL (123.17 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
In Vivo:
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (3.08 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
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Data Sheet (298 KB)
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SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
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Handling Instructions (2659 KB)
References
[2]. Helson L, et al. Cephalomannine and 10-deacetyltaxol cytotoxicity in human glial and neuroblastoma cell-lines. International journal of oncology. 1993 Feb;2(2):297-9. [Content Brief]
[3]. McLaughlin JL, et al. 19-Hydroxybaccatin III, 10-deacetylcephalomannine, and 10-deacetyltaxol: new antitumor taxanes from Taxus wallichiana. J Nat Prod. 1981 May-Jun;44(3):312-9. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 1.2317 mL | 6.1586 mL | 12.3172 mL | 30.7931 mL |
| 5 mM | 0.2463 mL | 1.2317 mL | 2.4634 mL | 6.1586 mL | |
| 10 mM | 0.1232 mL | 0.6159 mL | 1.2317 mL | 3.0793 mL | |
| 15 mM | 0.0821 mL | 0.4106 mL | 0.8211 mL | 2.0529 mL | |
| 20 mM | 0.0616 mL | 0.3079 mL | 0.6159 mL | 1.5397 mL | |
| 25 mM | 0.0493 mL | 0.2463 mL | 0.4927 mL | 1.2317 mL | |
| 30 mM | 0.0411 mL | 0.2053 mL | 0.4106 mL | 1.0264 mL | |
| 40 mM | 0.0308 mL | 0.1540 mL | 0.3079 mL | 0.7698 mL | |
| 50 mM | 0.0246 mL | 0.1232 mL | 0.2463 mL | 0.6159 mL | |
| 60 mM | 0.0205 mL | 0.1026 mL | 0.2053 mL | 0.5132 mL | |
| 80 mM | 0.0154 mL | 0.0770 mL | 0.1540 mL | 0.3849 mL | |
| 100 mM | 0.0123 mL | 0.0616 mL | 0.1232 mL | 0.3079 mL |
10-Deacetyltaxol Related Classifications
HY-N1391 Related Classifications
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