7α,25-Dihydroxycholesterol (Synonyms: 7α,25-OHC)

Name: 7α,25-Dihydroxycholesterol
Brand: MedChemExpress
SKU: HY-113962
Rating: 5.0 (3 reviews)

Cat. No.: HY-113962 Purity: 99.88%: Data Sheet
COA

SDS
Handling Instructions
FAQs
Technical Support

7α, 25-dihydroxycholesterol (7α,25-OHC) is a potent and selective agonist and endogenous ligand of the orphan GPCR receptor EBI2 (GPR183). 7α, 25-dihydroxycholesterol is highly potent at activating EBI2 (EC₅₀=140 pM; K_d=450 pM). 7α, 25-dihydroxycholesterol can serve as a chemokine directing migration of B cells, T cells and dendritic cells.

For research use only. We do not sell to patients.

CAS No. : 64907-22-8

Size	Stock	Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	In-stock	Estimated Time of Arrival: December 31
Solid
1 mg	In-stock	Estimated Time of Arrival: December 31
5 mg	In-stock	Estimated Time of Arrival: December 31
10 mg	In-stock	Estimated Time of Arrival: December 31
25 mg	In-stock	Estimated Time of Arrival: December 31
50 mg	In-stock	Estimated Time of Arrival: December 31
100 mg	In-stock	Estimated Time of Arrival: December 31
200 mg	Get quote
500 mg	Get quote

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Customer Review

Based on 3 publication(s) in Google Scholar

Other Forms of 7α,25-Dihydroxycholesterol:

7α,25-Dihydroxycholesterol-d₆ Get quote

3 Publications Citing Use of MCE 7α,25-Dihydroxycholesterol

Cell Proliferation/Viability Assay

Flow Cytometry

Cell Migration/Invasion Assay

: 7α,25-Dihydroxycholesterol purchased from MedChemExpress. Usage Cited in: Clin Exp Metastasis. 2025 Aug 4;42(5):44. [Abstract]; A549 and PC-9 cells were treated with 5 µM 7α,25-Dihydroxycholesterol (7α,25-OHC) for 24 h and/or 10 µM GSK682753A for 24 h and then examined for cell phenotypes. Cell viability was assessed using CCK-8 assays at 24-, 48-, and 72-hour after treatment.

: 7α,25-Dihydroxycholesterol purchased from MedChemExpress. Usage Cited in: Clin Exp Metastasis. 2025 Aug 4;42(5):44. [Abstract]; A549 and PC-9 cells were treated with 5 µM 7α,25-Dihydroxycholesterol (7α,25-OHC) for 24 h and/or 10 µM GSK682753A for 24 h and then examined for cell phenotypes. Cell apoptosis was quantified by flow cytometry using Annexin V-FITC/PI staining.

: 7α,25-Dihydroxycholesterol purchased from MedChemExpress. Usage Cited in: Clin Exp Metastasis. 2025 Aug 4;42(5):44. [Abstract]; A549 and PC-9 cells were treated with 5 µM 7α,25-Dihydroxycholesterol (7α,25-OHC) for 24 h and/or 10 µM GSK682753A for 24 h and then examined for cell phenotypes. Transwell migration assays were used to quantify cell migration at 24 hours.

: 7α,25-Dihydroxycholesterol purchased from MedChemExpress. Usage Cited in: Clin Exp Metastasis. 2025 Aug 4;42(5):44. [Abstract]; A549 cells were treated with 5 µM 7α,25-Dihydroxycholesterol (7α,25-OHC) for 24 h and/or 10 µM GSK682753A for 24 h and then examined for cell phenotypes. The protein levels of ERK and Akt pathways related proteins, including phosphorylated ERK1/2, total ERK1/2, phosphorylated Akt and total Akt, were analyzed by Immunoblotting.

: 7α,25-Dihydroxycholesterol purchased from MedChemExpress. Usage Cited in: Cell Metab. 2023 Nov 7;35(11):1915-1930.e8. [Abstract]; Transwell migration of CD7+ and CD7− monocytes transfected with si-Scramble or si-Gpr183 to 7α,25-Dihydroxycholesterol (7α,25-OHC).

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Biological Activity
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References
Customer Review

Description

IC₅₀ & Target^[1]

Human Endogenous Metabolite

In Vitro

In vitro, 7α, 25-dihydroxycholesterol (7α,25-OHC) stimulates the migration of EBI2-expressing mouse B and T cells with half-maximum effective concentration values around 500 pM, but had no effect on EBI2-deficient cells^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

In Vivo

EBI2-deficient B cells or normal B cells desensitized by 7α,25-Dihydroxycholesterol (1 μM; 1.5 hours) pre-treatment shows reduced homing to follicular areas of the spleen^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

418.65

Formula

C₂₇H₄₆O₃

CAS No.

64907-22-8

Appearance

Solid

Color

White to off-white

SMILES

C[C@@]12[C@](CC[C@]2([H])[C@H](C)CCCC(C)(O)C)([H])[C@@]3([H])[C@]([C@@]4(C(C[C@@H](O)CC4)=C[C@H]3O)C)([H])CC1

Structure Classification

Steroids

Initial Source

Endogenous metabolite

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
In solvent	-80°C	6 months
	-20°C	1 month

Solvent & Solubility

In Vitro:

DMSO : 4.55 mg/mL (10.87 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Ethanol : < 1 mg/mL (insoluble)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	2.3886 mL	11.9432 mL	23.8863 mL
5 mM	0.4777 mL	2.3886 mL	4.7773 mL
10 mM	0.2389 mL	1.1943 mL	2.3886 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 15% Cremophor EL 85% Saline
Solubility: 5 mg/mL (11.94 mM); Suspended solution; Need ultrasonic

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

Purity & Documentation

Purity: 99.88%

Select Batch:

Data Sheet (274 KB) SDS (252 KB)

COA (249 KB) HNMR (144 KB) RP-HPLC (238 KB)

Handling Instructions (2659 KB)

References

[1]. Liu C, et al. Oxysterols direct B-cell migration through EBI2. Nature. 2011 Jul 27;475(7357):519-23. [Content Brief]

[2]. Hannedouche S, et al. Oxysterols direct immune cell migration via EBI2. Nature. 2011 Jul 27;475(7357):524-7. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	2.3886 mL	11.9432 mL	23.8863 mL	59.7158 mL
	5 mM	0.4777 mL	2.3886 mL	4.7773 mL	11.9432 mL
	10 mM	0.2389 mL	1.1943 mL	2.3886 mL	5.9716 mL

7α,25-Dihydroxycholesterol Related Classifications

Inflammation/Immunology

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

7α,25-Dihydroxycholesterol64907-22-87α,25-OHCEBI2/GPR183Endogenous MetaboliteendogenousligandGPCRreceptorBcellsTimmuneresponsemigrationoxysterolsInhibitorinhibitorinhibit

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7α,25-Dihydroxycholesterol (Synonyms: 7α,25-OHC)

Customer Review

Other Forms of 7α,25-Dihydroxycholesterol:

7α,25-Dihydroxycholesterol Related Antibodies

3 Publications Citing Use of MCE 7α,25-Dihydroxycholesterol

Featured Recommendations

•Related Screening Libraries:

•Related Small Molecules:

•Related Proteins:

View All Endogenous Metabolite Isoform Specific Products:

Biological Activity

Purity & Documentation

References

Customer Review

7α,25-Dihydroxycholesterol Related Antibodies

Molarity Calculator

Dilution Calculator

Complete Stock Solution Preparation Table

7α,25-Dihydroxycholesterol Related Classifications

Keywords:

Your Recently Viewed Products:

Customer Review

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SAFETY DATA SHEET (SDS)

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