7α,25-Dihydroxycholesterol
Based on 5 publication(s) in Google Scholar
7α, 25-dihydroxycholesterol (7α,25-OHC) is a potent and selective agonist and endogenous ligand of the orphan GPCR receptor EBI2 (GPR183). 7α, 25-dihydroxycholesterol is highly potent at activating EBI2 (EC50=140 pM; Kd=450 pM). 7α, 25-dihydroxycholesterol can serve as a chemokine directing migration of B cells, T cells and dendritic cells.
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- Reinheit : 99.88%
- CAS. Nr.: 64907-22-8
- Formel: C27H46O3
- Molecular Weight:418.65
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Speicherung:Powder -20°C, 3 years ; In solvent -80°C, 6 months , -20°C, 1 month
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Biologische Aktivität
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Chemical Information
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Lösungsmittel & Löslichkeit
- Protokoll
- Reinheit & Dokumentation
- Verweise
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Help & FAQs
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Publications Citing Use of MedChemExpress (MCE) 7α,25-Dihydroxycholesterol
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Customer Validation & Images
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Cell Proliferation/Viability Assay
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Flow Cytometry
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Cell Migration/Invasion Assay
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WB
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Cell Migration/Invasion Assay
Alle Endogenous Metabolite Isoform-spezifische Produkte anzeigen
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Biologische Aktivität
Beschreibung
IC50 & Target
[1]|
Human Endogenous Metabolite |
In Vitro
In vitro, 7α, 25-dihydroxycholesterol (7α,25-OHC) stimulates the migration of EBI2-expressing mouse B and T cells with half-maximum effective concentration values around 500 pM, but had no effect on EBI2-deficient cells[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. Further protocols information, click here.
In Vivo
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS. Nr. 64907-22-8
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Appearance Solid
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Molecular Weight 418.65
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Formel C27H46O3
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Color White to off-white
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SMILES
C[C@@]12[C@](CC[C@]2([H])[C@H](C)CCCC(C)(O)C)([H])[C@@]3([H])[C@]([C@@]4(C(C[C@@H](O)CC4)=C[C@H]3O)C)([H])CC1
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Synonyms
7α,25-OHC
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Structure Classification
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Initial Source
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Versand
Room temperature in continental US; may vary elsewhere.
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Speicherung
Powder -20°C 3 years In solvent -80°C 6 months -20°C 1 month
Publications (5)
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Journal Impact Factor
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Most Recent
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Cell Metab
Bone marrow immune cells respond to fluctuating nutritional stress to constrain weight regain. [Abstract]2023 Nov 7;35(11):1915-1930.e8. PMID: 37703873
7α,25-Dihydroxycholesterol purchased from MedChemExpress. Usage Cited in: Cell Metab. 2023 Nov 7;35(11):1915-1930.e8. [Abstract]
Transwell migration of CD7+ and CD7− monocytes transfected with si-Scramble or si-Gpr183 to 7α,25-Dihydroxycholesterol (7α,25-OHC).
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Adv Sci (Weinh)
Natural Resistance to Ovarian Hyperstimulation Syndrome in Estrildid Finches Reveals Macrophage GPR183 as a Potential Therapeutic Target. [Abstract]2026 May 5:e75523. PMID: 42085637 -
J Biol Chem
2024 Dec;300(12):107945. PMID: 39505210 -
Clin Exp Metastasis
G-protein coupled receptor 183 (GPR183) inhibits visceral metastasis of non-small cell lung cancer. [Abstract]2025 Aug 4;42(5):44. PMID: 40759817
7α,25-Dihydroxycholesterol purchased from MedChemExpress. Usage Cited in: Clin Exp Metastasis. 2025 Aug 4;42(5):44. [Abstract]
A549 and PC-9 cells were treated with 5 µM 7α,25-Dihydroxycholesterol (7α,25-OHC) for 24 h and/or 10 µM GSK682753A for 24 h and then examined for cell phenotypes. Cell viability was assessed using CCK-8 assays at 24-, 48-, and 72-hour after treatment.
7α,25-Dihydroxycholesterol purchased from MedChemExpress. Usage Cited in: Clin Exp Metastasis. 2025 Aug 4;42(5):44. [Abstract]
A549 and PC-9 cells were treated with 5 µM 7α,25-Dihydroxycholesterol (7α,25-OHC) for 24 h and/or 10 µM GSK682753A for 24 h and then examined for cell phenotypes. Cell apoptosis was quantified by flow cytometry using Annexin V-FITC/PI staining.
7α,25-Dihydroxycholesterol purchased from MedChemExpress. Usage Cited in: Clin Exp Metastasis. 2025 Aug 4;42(5):44. [Abstract]
A549 and PC-9 cells were treated with 5 µM 7α,25-Dihydroxycholesterol (7α,25-OHC) for 24 h and/or 10 µM GSK682753A for 24 h and then examined for cell phenotypes. Transwell migration assays were used to quantify cell migration at 24 hours.
7α,25-Dihydroxycholesterol purchased from MedChemExpress. Usage Cited in: Clin Exp Metastasis. 2025 Aug 4;42(5):44. [Abstract]
A549 cells were treated with 5 µM 7α,25-Dihydroxycholesterol (7α,25-OHC) for 24 h and/or 10 µM GSK682753A for 24 h and then examined for cell phenotypes. The protein levels of ERK and Akt pathways related proteins, including phosphorylated ERK1/2, total ERK1/2, phosphorylated Akt and total Akt, were analyzed by Immunoblotting.
Lösungsmittel & Löslichkeit
In Vitro:
DMSO : 4.55 mg/mL (10.87 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Ethanol : < 1 mg/mL (insoluble)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Konzentration (Stammlösung) × Volumen (Stammlösung) = Konzentration (Ziellösung) × Volumen (Ziellösung)
In Vivo:
For the following dissolution methods, please prepare the working solution directly:
It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 15% Cremophor EL 85% Saline
Solubility: 5 mg/mL (11.94 mM); Suspended solution; Need ultrasonic
Protokoll
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Cell migration
Cell migration is a method that plays an important role in wound healing, cell differentiation, embryonic development, etc.
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Monocyte-derived dendritic cell differentiation
Human monocyte-derived dendritic cells are generated by isolating PBMC-derived monocytes and culturing them with GM-CSF plus IL-4, which produces cells with dendritic-cell antigen-presenting properties, reduced monocyte phenotype, and increased dendritic-cell functional readouts such as antigen uptake, allogeneic T-cell stimulation, and expression of markers including HLA-DR, CD80, CD86, CD83, CD1a, or CD209 depending on protocol and maturation state. The main readout is phenotypic and functional differentiation: immature MoDCs are commonly evaluated by loss or reduction of CD14 with acquisition of dendritic-cell markers and antigen uptake capacity, whereas mature MoDCs are evaluated by increased CD83, CD80, CD86, HLA-DR, and T-cell stimulatory function after exposure to maturation stimuli such as TNF-α or a cytokine/PGE2 cocktail.
Reinheit & Dokumentation
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Data Sheet (274 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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Handling Instructions (2659 KB)
Verweise
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
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| DMSO | 1 mM | 2.3886 mL | 11.9432 mL | 23.8863 mL | 59.7158 mL |
| 5 mM | 0.4777 mL | 2.3886 mL | 4.7773 mL | 11.9432 mL | |
| 10 mM | 0.2389 mL | 1.1943 mL | 2.3886 mL | 5.9716 mL |
7α,25-Dihydroxycholesterol Related Classifications
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