1. Membrane Transporter/Ion Channel
  2. Na+/K+ ATPase
  3. Bufalin

Bufalin 

Cat. No.: HY-N0877 Purity: 98.85%
Handling Instructions

Bufalin is an active component isolated from Chan Su, acts as a potent Na+/K+-ATPase inhibitor, binds to the subunit α1, α2 and α3, with Kd of 42.5, 45 and 40 nM, respectively. Anti-cancer activity.

For research use only. We do not sell to patients.

Bufalin Chemical Structure

Bufalin Chemical Structure

CAS No. : 465-21-4

Size Price Stock Quantity
10 mM * 1 mL in DMSO USD 178 In-stock
Estimated Time of Arrival: December 31
5 mg USD 162 In-stock
Estimated Time of Arrival: December 31
10 mg USD 306 In-stock
Estimated Time of Arrival: December 31
50 mg   Get quote  
100 mg   Get quote  

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Based on 1 publication(s) in Google Scholar

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Description

Bufalin is an active component isolated from Chan Su, acts as a potent Na+/K+-ATPase inhibitor, binds to the subunit α1, α2 and α3, with Kd of 42.5, 45 and 40 nM, respectively[1][2]. Anti-cancer activity[2].

IC50 & Target

Kd: 42.5 nM (Na,K-ATPase α1), 45 nM (Na,K-ATPase α2), 40 nM (Na,K-ATPase α3)[1]

In Vitro

Bufalin (0, 1, 2, 4 μM for 48 hours) decreases cell viability in NCI-H460 cells[2].
Bufalin (2 μM) increases caspae-3, Endo G and GADD153 mRNA expression, but decreases the GRP78 mRNA expression[2].

Cell Viability Assay[2]

Cell Line: NCI-H460 cells
Concentration: 0, 1, 2, 4 μM
Incubation Time: 48 hours
Result: Decreased viability of NCI-H460 cells in a dose-dependent manner.
In Vivo

Bufalin (0.1, 0.2, or 0.4 mg/kg, i.p., daily for 14 days) shows significant anti-tumor activity in mice bearing NCI-H460 cells[2].

Animal Model: Forty male athymic BALB/c nu/nu mice (6-8 weeks old)[2]
Dosage: 0.1, 0.2, or 0.4 mg/kg
Administration: I.P. every day until 14 days
Result: Dose-dependently suppressed tumor growth.
Molecular Weight

386.52

Formula

C₂₄H₃₄O₄

CAS No.

465-21-4

SMILES
Shipping

Room temperature in continental US; may vary elsewhere.

Storage
Powder -20°C 3 years
  4°C 2 years
In solvent -80°C 6 months
  -20°C 1 month
Solvent & Solubility
In Vitro: 

DMSO : 100 mg/mL (258.72 mM; Need ultrasonic)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.5872 mL 12.9359 mL 25.8719 mL
5 mM 0.5174 mL 2.5872 mL 5.1744 mL
10 mM 0.2587 mL 1.2936 mL 2.5872 mL
*Please refer to the solubility information to select the appropriate solvent.
In Vivo:
  • 1.

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% saline

    Solubility: ≥ 2.08 mg/mL (5.38 mM); Clear solution

  • 2.

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in saline)

    Solubility: ≥ 2.08 mg/mL (5.38 mM); Clear solution

  • 3.

    Add each solvent one by one:  10% DMSO    90% corn oil

    Solubility: ≥ 2.08 mg/mL (5.38 mM); Clear solution

*All of the co-solvents are provided by MCE.
References

Purity: 98.85%

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Bufalin
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HY-N0877
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