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DIM-C-pPhOCH3 

Cat. No.: HY-111492 Purity: 99.30%
Handling Instructions

DIM-C-pPhOCH3 is a Nur77 agonist. Nerve growth factor-induced Bα (NGFI-Bα, Nur77) is an orphan nuclear receptor.

For research use only. We do not sell to patients.

DIM-C-pPhOCH3 Chemical Structure

DIM-C-pPhOCH3 Chemical Structure

CAS No. : 33985-68-1

Size Price Stock Quantity
10 mM * 1 mL in DMSO USD 88 In-stock
Estimated Time of Arrival: December 31
5 mg USD 80 In-stock
Estimated Time of Arrival: December 31
10 mg USD 120 In-stock
Estimated Time of Arrival: December 31
25 mg USD 260 In-stock
Estimated Time of Arrival: December 31
50 mg USD 410 In-stock
Estimated Time of Arrival: December 31
100 mg   Get quote  
200 mg   Get quote  

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Customer Review

Based on 1 publication(s) in Google Scholar

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Description

DIM-C-pPhOCH3 is a Nur77 agonist. Nerve growth factor-induced Bα (NGFI-Bα, Nur77) is an orphan nuclear receptor.

IC50 & Target

Nur77[1]

In Vitro

DIM-C-pPhOCH3 decreases survival and induces apoptosis in RKO colon cancer cells, and this is accompanied by induction of tumor necrosis factor–related apoptosis-inducing ligand (TRAIL) protein. DIM-C-pPhOCH3 also induces Nur77-independent apoptosis. DIM-C-pPhOCH3 (10 μM) inhibits cell growth after treatment for 24, 48, or 72 h, and the maximum inhibitory response is observed after 72 h, where there is considerable cell detachment and dead cells. The growth-inhibitory effects observed for DIM-C-pPhOCH3 after 72 h are also accompanied by several markers of apoptosis, including PARP cleavage and cleavage of caspase-3, caspase-9, and caspase-8. PARP cleavage is also observed after treatment of RKO cells for 48 h with DIM-C-pPhOCH3[1].

In Vivo

DIM-C-pPhOCH3 (25 mg/kg/d) also inhibits tumor growth in athymic nude mice bearing RKO cell xenografts. The effects of DIM-C-pPhOCH3 (25 mg/kg/d) on colon tumor growth are also investigated in athymic nude mice bearing RKO cell xenografts. Treatment with the DIM-C-pPhOCH3 significantly decreases tumor volumes and final tumor weights compared with corn oil controls[1].

Molecular Weight

352.43

Formula

C₂₄H₂₀N₂O

CAS No.

33985-68-1

SMILES

COC1=CC=C(C(C2=CNC3=C2C=CC=C3)C4=CNC5=C4C=CC=C5)C=C1

Shipping

Room temperature in continental US; may vary elsewhere.

Storage
Powder -20°C 3 years
  4°C 2 years
In solvent -80°C 6 months
  -20°C 1 month
Solvent & Solubility
In Vitro: 

DMSO : ≥ 33.3 mg/mL (94.49 mM)

*"≥" means soluble, but saturation unknown.

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.8374 mL 14.1872 mL 28.3744 mL
5 mM 0.5675 mL 2.8374 mL 5.6749 mL
10 mM 0.2837 mL 1.4187 mL 2.8374 mL
*Please refer to the solubility information to select the appropriate solvent.
In Vivo:
  • 1.

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% saline

    Solubility: ≥ 2.5 mg/mL (7.09 mM); Clear solution

  • 2.

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in saline)

    Solubility: ≥ 2.5 mg/mL (7.09 mM); Clear solution

*All of the co-solvents are provided by MCE.
References
Cell Assay
[1]

RKO cells are treated with DMSO or 12.5 μM DIM-C-pPhOCH3 for 2 and 6 h. RNA is isolated for the reverse transcription-PCR (RT-PCR) experiment and analyzed for gene expression, and three replicates are determined for each time point and the DMSO control. The microarray data are analyzed[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Administration
[1]

Mice[1]
Male athymic nude mice (Foxn1nu, ages 7-8 weeks) are used. The mice are housed and maintained in laminar flow cabinets under specific pathogen-free conditions. A xenograft is established by s.c. injection of in vitro cultured RKO cells (5×106 per 150 μL) into the flanks of individual mice. Tumors are allowed to grow for 4 days until tumors are palpable. Mice are then randomized into two groups of six mice per group and dosed by oral gavage with either corn oil or 25 mg/kg/d DIM-C-pPhOCH3 for 21 days. The mice are weighed, and tumor size is measured[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

References
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DIM-C-pPhOCH3
Cat. No.:
HY-111492
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