Dobutamine hydrochloride
Based on 5 publication(s) in Google Scholar
Dobutamine hydrochloride is a synthetic catecholamine that acts on α1-AR, β1-AR, β2-AR (α-1, β-1 andβ-2 adrenoceptors). Dobutamine hydrochloride is a selective β1-AR agonist, relatively weak activity at α1-AR and β2-AR. Dobutamine hydrochloride can increase cardiac output and correct hypoperfusion.
For research use only. We do not sell to patients.
- Purity: 99.60%
- CAS No.: 49745-95-1
- Formula: C18H24ClNO3
- Molecular Weight:337.84
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Storage:
4°C, sealed storage, away from moisture
* In solvent : -80°C, 2 years; -20°C, 1 year (sealed storage, away from moisture)
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Publications Citing Use of MedChemExpress (MCE) Dobutamine hydrochloride
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All Adrenergic Receptor Isoforms
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Biological Activity
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α adrenergic receptor |
β adrenergic receptor |
Dobutamine hydrochloride (0.15-20 mg/kg; i.p.) results in subsequent increase in the left ventricular function and heart rate acceleration with an increasing dose in wildtype mice[3].
Dobutamine hydrochloride results in significant inotropic, lusitropic, and chronotropic cardiac response with a high dose in wildtype mice[3].
Low doses of Dobutamine hydrochloride significantly increases inotropic and lusitropic cardiac performance without chronotropic changes in the Tgαq*44 mice[3].
Dobutamine hydrochloride increases heart rate only after high doses, but then inotropic and lusitropic cardiac functional reserve is lost[3].
Dobutamine hydrochloride increases alveolar liquid clearance in ventilated rats by beta-2 receptor stimulation[4].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:Tgαq*44 mice (heart failure models)[3]
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Dosage:0.15 mg/kg, 0.5 mg/kg as a low dose, 1.5 mg/kg, 5 mg/kg, 20 mg/kg as a high dose
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Administration:Intraperitoneal injection
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Result:Induced different response in cardiac function on a low and high dose in mice with with heart failure.
Chemical Information
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CAS No. 49745-95-1
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Appearance Solid
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Molecular Weight 337.84
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Formula C18H24ClNO3
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Color White to gray
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SMILES
OC1=CC=C(CCNC(C)CCC2=CC=C(O)C=C2)C=C1O.[H]Cl
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
4°C, sealed storage, away from moisture
* In solvent : -80°C, 2 years; -20°C, 1 year (sealed storage, away from moisture)
Publications (5)
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Journal Impact Factor
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Most Recent
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Anal Chem
2025 Jul 15;97(27):14220-14229. PMID: 40601392 -
Biochem Pharmacol
2025 Nov 29:244:117576. PMID: 41319924 -
Front Cell Dev Biol
2022 Apr 20;10:889656. PMID: 35517499
Dobutamine hydrochloride purchased from MedChemExpress. Usage Cited in: Front Cell Dev Biol. 2022 Apr 20;10:889656. [Abstract]
Dobutamine hydrochloride (dobutamine; 10 μM; 18 h; 10 Gy IR) decreased IR-induced cell apoptosis. Ⅰ: control cells without IR treatment, Ⅱ: control cells with IR treatment, Ⅲ: dobutamine pre-treated control cells with IR treatment, Ⅳ: BRCA1 knock-down cells with IR treatment, Ⅴ: dobutamine pre-treated BRCA1 knock-down cells with IR treatment, Ⅵ: 8-CPT-cAMP pre-treated BRCA1 knock-downcells with IR treatment.
Dobutamine hydrochloride purchased from MedChemExpress. Usage Cited in: Front Cell Dev Biol. 2022 Apr 20;10:889656. [Abstract]
Dobutamine hydrochloride (dobutamine; 10 μM; 18 h; 10 Gy IR) decreased IR-induced cell apoptosis. Ⅰ: control cells without IR treatment, Ⅱ: control cells with IR treatment, Ⅲ: dobutamine pre-treated control cells with IR treatment, Ⅳ: BRCA1 knock-down cells with IR treatment, Ⅴ: dobutamine pre-treated BRCA1 knock-down cells with IR treatment, Ⅵ: 8-CPT-cAMP pre-treated BRCA1 knock-down cells with IR treatment.
Dobutamine hydrochloride purchased from MedChemExpress. Usage Cited in: Front Cell Dev Biol. 2022 Apr 20;10:889656. [Abstract]
Dobutamine hydrochloride (dobutamine; 10 μM; 18 h; 10 Gy IR) showed the percentages of G1 cells in IRtreated control and BRCA1 knock-down A2780 cells decreased to 44.32 and 43.03%, respectively, suggesting that cAMP terminated the cell cycle arrest caused by DNA damage. Ⅰ: control cells without IR treatment, Ⅱ: control cells with IR treatment, Ⅲ: dobutamine pre-treated control cells with IR treatment, Ⅳ: BRCA1 knock-down cells with IR treatment, Ⅴ: dobutamine pre-treated BRCA1 knock-down cells with IR treatment, Ⅵ: 8-CPT-cAMP pre-treated BRCA1 knock-down cells with IR treatment.
Dobutamine hydrochloride purchased from MedChemExpress. Usage Cited in: Front Cell Dev Biol. 2022 Apr 20;10:889656. [Abstract]
Dobutamine hydrochloride (dobutamine; 10 μM) treated BRCA1 knock-down A2780 cells could inhibited the proliferation of the CD8+ T cells.
Dobutamine hydrochloride purchased from MedChemExpress. Usage Cited in: Front Cell Dev Biol. 2022 Apr 20;10:889656. [Abstract]
Dobutamine hydrochloride (dobutamine; 10 μM; 18 h after IR) showed weaker BAX signals in the IR-irradiated control and BRCA1 knockdown A2780 cells, and 13.7 and 14.5% of cancer cells were BAX positive, respectively.
Dobutamine hydrochloride purchased from MedChemExpress. Usage Cited in: Front Cell Dev Biol. 2022 Apr 20;10:889656. [Abstract]
Dobutamine hydrochloride (dobutamine; 10 μM; 18 h after IR) decreased the protein expression of BAX and cleaved caspase-3 in the IR-irradiated control and BRCA1 knockdown A2780 cells.
Dobutamine hydrochloride purchased from MedChemExpress. Usage Cited in: Front Cell Dev Biol. 2022 Apr 20;10:889656. [Abstract]
Dobutamine hydrochloride (dobutamine; 10 μM; 18 h after IR) decreased the protein expression of p53 in the IR-irradiated BRCA1 knockdown A2780 cells.
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Comput Struct Biotechnol J
Drug repurposing screens to identify potential drugs for chronic kidney disease by targeting prostaglandin E2 receptor. [Abstract]2023 Jul 7:21:3490-3502. PMID: 37484490 -
Environ Toxicol
Phenylacetyl glutamine (PAGln) enhances cardiomyocyte death after myocardial infarction through β1 adrenergic receptor. [Abstract]2024 Mar;39(3):1682-1699. PMID: 38041472
Solvent & Solubility
DMSO : ≥ 33 mg/mL (97.68 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
H2O : 20 mg/mL (59.20 mM; Need ultrasonic)
* "≥" means soluble, but saturation unknown.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year (sealed storage, away from moisture). When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year (sealed storage, away from moisture). When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (7.40 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (7.40 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
For the following dissolution methods, please prepare the working solution directly:
It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: PBS
Solubility: 16.67 mg/mL (49.34 mM); Clear solution; Need ultrasonic
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Working solution concentration: 0.22 mg/mL
This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
Purity & Documentation
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Data Sheet (273 KB)
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SDS (418 KB)
- English - EN (418 KB)
- Français - FR (418 KB)
- Deutsch - DE (418 KB)
- Norwegian - NO (418 KB)
- Español - ES (418 KB)
- Swedish - SV (418 KB)
- Italian - IT (418 KB)
- Korean - KR (418 KB)
- Portuguese - PT (418 KB)
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Handling Instructions (2659 KB)
References
[1]. Tuttle RR, et al. Dobutamine: development of a new catecholamine to selectively increase cardiac contractility. Circ Res. 1975 Jan;36(1):185-96. [Content Brief]
[2]. Vallet B, et al. Dobutamine: mechanisms of action and use in acute cardiovascular pathology. Ann Cardiol Angeiol (Paris). 1991 Jun;40(6):397-402. [Content Brief]
[3]. Tyrankiewicz U , et al. Characterization of the cardiac response to a low and high dose of dobutamine in the mouse model of dilated cardiomyopathy by MRI in vivo. J Magn Reson Imaging. 2013 Mar;37(3):669-77. [Content Brief]
[4]. Tibayan FA, et al. Dobutamine increases alveolar liquid clearance in ventilated rats by beta-2 receptor stimulation. Am J Respir Crit Care Med. 1997 Aug;156(2 Pt 1):438-44. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year (sealed storage, away from moisture). When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| H2O / DMSO | 1 mM | 2.9600 mL | 14.7999 mL | 29.5998 mL | 73.9995 mL |
| 5 mM | 0.5920 mL | 2.9600 mL | 5.9200 mL | 14.7999 mL | |
| 10 mM | 0.2960 mL | 1.4800 mL | 2.9600 mL | 7.4000 mL | |
| 15 mM | 0.1973 mL | 0.9867 mL | 1.9733 mL | 4.9333 mL | |
| 20 mM | 0.1480 mL | 0.7400 mL | 1.4800 mL | 3.7000 mL | |
| 25 mM | 0.1184 mL | 0.5920 mL | 1.1840 mL | 2.9600 mL | |
| 30 mM | 0.0987 mL | 0.4933 mL | 0.9867 mL | 2.4667 mL | |
| 40 mM | 0.0740 mL | 0.3700 mL | 0.7400 mL | 1.8500 mL | |
| 50 mM | 0.0592 mL | 0.2960 mL | 0.5920 mL | 1.4800 mL | |
| DMSO | 60 mM | 0.0493 mL | 0.2467 mL | 0.4933 mL | 1.2333 mL |
| 80 mM | 0.0370 mL | 0.1850 mL | 0.3700 mL | 0.9250 mL |
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Dobutamine hydrochloride Related Classifications
HY-15746 Related Classifications
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