2. GPCR/G Protein MAPK/ERK Pathway
  3. Ras
  4. EPAC 5376753

EPAC 5376753 is an allosterically inhibitor of Epac which inhibits Epac1 with an IC50 of 4 µM in Swiss 3T3 cells.

For research use only. We do not sell to patients.

EPAC 5376753 Chemical Structure

EPAC 5376753 Chemical Structure

CAS No. : 302826-61-5

Size Price Stock Quantity
Solid + Solvent
10 mM * 1 mL in DMSO
ready for reconstitution
 USD 140 In-stock
Solution
10 mM * 1 mL in DMSO USD 140 In-stock
Solid
5 mg USD 128 In-stock
10 mg USD 193 In-stock
25 mg USD 386 In-stock
50 mg USD 580 In-stock
100 mg USD 868 In-stock
200 mg   Get quote  
500 mg   Get quote  

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Based on 1 publication(s) in Google Scholar

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Description

EPAC 5376753 is an allosterically inhibitor of Epac which inhibits Epac1 with an IC50 of 4 µM in Swiss 3T3 cells.

IC50 & Target

IC50: 4 µM (Epac)[1]
In Vitro

Epac is a key mediator of cAMP signaling. EPAC 5376753, the thiobarbituric acid modification of 5225554, has a greater ability to decrease the conformational changes of Epac necessary for its activation and to inhibit Epac signaling in cells. 5376753 selectively inhibits Epac-mediated migration in fibroblasts[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

EPAC 5376753 Related Antibodies
Molecular Weight

367.21

Formula

C15H8Cl2N2O3S
CAS No.
Appearance

Solid

Color

Orange to red

SMILES

O=C(/C(C(N1)=O)=C/C2=CC=C(C3=CC=C(Cl)C=C3Cl)O2)NC1=S
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

4°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Solvent & Solubility
In Vitro: 

DMSO : 10 mg/mL (27.23 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.7232 mL 13.6162 mL 27.2324 mL
5 mM 0.5446 mL 2.7232 mL 5.4465 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

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In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
Purity & Documentation
References
Cell Assay
[1]

Swiss 3T3 cells are treated with EPAC 5376753 (1, 10, 25, 50, 75, 100 μM) or 1% dimethyl sulfoxide for 48 h. Cell viability is assessed using the CellTiter-Glo assay[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 2.7232 mL 13.6162 mL 27.2324 mL 68.0809 mL
5 mM 0.5446 mL 2.7232 mL 5.4465 mL 13.6162 mL
10 mM 0.2723 mL 1.3616 mL 2.7232 mL 6.8081 mL
15 mM 0.1815 mL 0.9077 mL 1.8155 mL 4.5387 mL
20 mM 0.1362 mL 0.6808 mL 1.3616 mL 3.4040 mL
25 mM 0.1089 mL 0.5446 mL 1.0893 mL 2.7232 mL
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EPAC 5376753 Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

EPAC 5376753302826-61-5EPAC5376753EPAC-5376753RasInhibitorinhibitorinhibit

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