Cat. No.: HY-15382

FTY720 (S)-Phosphate

(Synonyms: (S)-FTY720P; (S)-FTY720 phosphate)

3 Cited Publications

Customer Review

Based on 3 publication(s) in Google Scholar

FTY720 (S)-Phosphate is an agonist of S1P receptor 1 (S1PR1), used in the research of acute inflammatory diseases such as acute lung injury.

For research use only. We do not sell to patients.

Purity: 99.57%
CAS No.: 402616-26-6
Formula: C₁₉H₃₄NO₅P
Molecular Weight:387.45

Storage:

Powder -20°C, 3 years

* The compound is unstable in solutions, freshly prepared is recommended.

FTY720 (S)-Phosphate Chemical Structure

CAS No.: 402616-26-6

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Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biological Activity
- Description
- IC₅₀ & Target
- Cellular Effect
- In Vitro
- In Vivo
Chemical Information
Publications (3)
Solvent & Solubility
- In Vitro
- In Vivo
Protocol
Purity & Documentation
References
Help & FAQs
Classifications
Bioz

Biological Activity

Description

FTY720 (S)-Phosphate is an agonist of S1P receptor 1 (S1PR1), used in the research of acute inflammatory diseases such as acute lung injury.

IC₅₀ & Target

S1PR1^[1]

All LPL Receptor Isoforms :

Cellular Effect

Cell Line	Type	Value	Description	References
CHO	EC₅₀	0.3 nM Compound: (S)-5	Agonism of human S1P-1 receptor expressed in CHO cells, 90-120 min in pH 7.4 using [35S]GTP-gamma-S as radioligand Agonism of human S1P-1 receptor expressed in CHO cells, 90-120 min in pH 7.4 using [35S]GTP-gamma-S as radioligand	[PMID: 16078855]
CHO	EC₅₀	0.3 nM Compound: (S)-5	Agonism of human S1P-5 receptor expressed in CHO cells, 90-120 min in pH 7.4 using [35S]GTP-gamma-S as radioligand Agonism of human S1P-5 receptor expressed in CHO cells, 90-120 min in pH 7.4 using [35S]GTP-gamma-S as radioligand	[PMID: 16078855]
CHO	EC₅₀	0.6 nM Compound: (S)-5	Agonism of human S1P-4 receptor expressed in CHO cells, 90-120 min in pH 7.4 using [35S]GTP-gamma-S as radioligand Agonism of human S1P-4 receptor expressed in CHO cells, 90-120 min in pH 7.4 using [35S]GTP-gamma-S as radioligand	[PMID: 16078855]
CHO	EC₅₀	3.1 nM Compound: (S)-5	Agonism of human S1P-3 receptor expressed in CHO cells, 90-120 min in pH 7.4 using [35S]GTP-gamma-S as radioligand Agonism of human S1P-3 receptor expressed in CHO cells, 90-120 min in pH 7.4 using [35S]GTP-gamma-S as radioligand	[PMID: 16078855]
CHO	EC₅₀	>10000 nM Compound: (S)-5	Agonism of human S1P-2 receptor expressed in CHO cells, 90-120 min in pH 7.4 using [35S]GTP-gamma-S as radioligand Agonism of human S1P-2 receptor expressed in CHO cells, 90-120 min in pH 7.4 using [35S]GTP-gamma-S as radioligand	[PMID: 16078855]
CHO	IC₅₀	2.1 nM Compound: (S)-FTY720-P, (S)-FTY720-Phosphate	Agonist activity at human S1P1 expressed in CHO cells after 60 mins by [35S]GTPgammaS binding assay Agonist activity at human S1P1 expressed in CHO cells after 60 mins by [35S]GTPgammaS binding assay	[PMID: 22119341]
CHO	IC₅₀	2.2 nM Compound: (S)-FTY720-P, (S)-FTY720-Phosphate	Agonist activity at human S1P4 expressed in CHO cells after 60 mins by [35S]GTPgammaS binding assay Agonist activity at human S1P4 expressed in CHO cells after 60 mins by [35S]GTPgammaS binding assay	[PMID: 22119341]
CHO	IC₅₀	23 nM Compound: (S)-FTY720-P, (S)-FTY720-Phosphate	Agonist activity at human S1P3 expressed in CHO cells after 60 mins by [35S]GTPgammaS binding assay Agonist activity at human S1P3 expressed in CHO cells after 60 mins by [35S]GTPgammaS binding assay	[PMID: 22119341]
CHO	IC₅₀	5.9 nM Compound: (S)-FTY720-P, (S)-FTY720-Phosphate	Agonist activity at human S1P2 expressed in CHO cells after 60 mins by [35S]GTPgammaS binding assay Agonist activity at human S1P2 expressed in CHO cells after 60 mins by [35S]GTPgammaS binding assay	[PMID: 22119341]
CHO	IC₅₀	0.28 nM Compound: FTY-720-P	Displacement of [33P]S1P from human S1P1R expressed in CHO cell membranes Displacement of [33P]S1P from human S1P1R expressed in CHO cell membranes	[PMID: 24900286]
CHO	IC₅₀	6.3 nM Compound: FTY-720-P	Displacement of [33P]S1P from human S1P3R expressed in CHO cell membranes Displacement of [33P]S1P from human S1P3R expressed in CHO cell membranes	[PMID: 24900286]
CHO	EC₅₀	0.28 nM Compound: 2	Induction of internalization of C-terminal GFP-fused human S1P1 receptor expressed in CHO cell membranes after 50 mins Induction of internalization of C-terminal GFP-fused human S1P1 receptor expressed in CHO cell membranes after 50 mins	[PMID: 26924461]
CHO-K1	EC₅₀	0.027 μM Compound: 2a, FTY720-P	Agonist activity at human S1P3 receptor expressed in CHO-K1 cells co-expressing Gq/i5 G-protein assessed as calcium mobilization by FLIPR assay Agonist activity at human S1P3 receptor expressed in CHO-K1 cells co-expressing Gq/i5 G-protein assessed as calcium mobilization by FLIPR assay	[PMID: 22104144]
CHO-K1	EC₅₀	0.29 nM Compound: 2, FTY720-P	Agonist activity at rat S1P1 receptor expressed in CHO-K1 cells assessed as induction of GTPgammaS binding Agonist activity at rat S1P1 receptor expressed in CHO-K1 cells assessed as induction of GTPgammaS binding	[PMID: 22405291]
CHO-K1	EC₅₀	0.37 nM Compound: 2, FTY720-P	Agonist activity at human S1P1 receptor expressed in CHO-K1 cells assessed as induction of GTPgammaS binding Agonist activity at human S1P1 receptor expressed in CHO-K1 cells assessed as induction of GTPgammaS binding	[PMID: 22405291]
CHO-K1	EC₅₀	1.3 nM Compound: 2, FTY720-P	Agonist activity at rat S1P3 receptor expressed in CHO-K1 cells assessed as induction of GTPgammaS binding Agonist activity at rat S1P3 receptor expressed in CHO-K1 cells assessed as induction of GTPgammaS binding	[PMID: 22405291]
CHO-K1	EC₅₀	3.3 nM Compound: 2, FTY720-P	Agonist activity at human S1P3 receptor expressed in CHO-K1 cells assessed as induction of GTPgammaS binding Agonist activity at human S1P3 receptor expressed in CHO-K1 cells assessed as induction of GTPgammaS binding	[PMID: 22405291]
CHO-K1	EC₅₀	0.29 nM Compound: 2, FTY720-P	Agonist activity at rat S1P1 receptor expressed in CHO-K1 cells by [35S]GTPgammaS binding assay Agonist activity at rat S1P1 receptor expressed in CHO-K1 cells by [35S]GTPgammaS binding assay	[PMID: 24900318]
CHO-K1	EC₅₀	0.37 nM Compound: 2, FTY720-P	Agonist activity at human S1P1 receptor expressed in CHO-K1 cells by [35S]GTPgammaS binding assay Agonist activity at human S1P1 receptor expressed in CHO-K1 cells by [35S]GTPgammaS binding assay	[PMID: 24900318]
CHO-K1	EC₅₀	1.3 nM Compound: 2, FTY720-P	Agonist activity at rat S1P3R expressed in CHO-K1 cells by [35S]GTPgammaS binding assay Agonist activity at rat S1P3R expressed in CHO-K1 cells by [35S]GTPgammaS binding assay	[PMID: 24900318]
CHO-K1	EC₅₀	3.3 nM Compound: 2, FTY720-P	Agonist activity at human S1P3R expressed in CHO-K1 cells by [35S]GTPgammaS binding assay Agonist activity at human S1P3R expressed in CHO-K1 cells by [35S]GTPgammaS binding assay	[PMID: 24900318]
CHO-K1	EC₅₀	2 nM Compound: 1-P	Agonist activity at human S1P3 receptor expressed in CHOK1 cell membranes by [35S]GTPgammaS binding assay Agonist activity at human S1P3 receptor expressed in CHOK1 cell membranes by [35S]GTPgammaS binding assay	[PMID: 24909680]
CHO-K1	EC₅₀	7 nM Compound: 1-P	Agonist activity at human S1P1 receptor expressed in CHOK1 cell membranes by [35S]GTPgammaS binding assay Agonist activity at human S1P1 receptor expressed in CHOK1 cell membranes by [35S]GTPgammaS binding assay	[PMID: 24909680]
U2OS	EC₅₀	0.4 nM Compound: FTY720P	Agonist activity at human S1P1 receptor expressed in EDG1-bla U2OS cells incubated for 18 hrs prior to GenBlazer substrate addition by beta-arrestin recruitment assay Agonist activity at human S1P1 receptor expressed in EDG1-bla U2OS cells incubated for 18 hrs prior to GenBlazer substrate addition by beta-arrestin recruitment assay	[PMID: 26687487]
U2OS	EC₅₀	94 nM Compound: FTY720P	Agonist activity at human S1P3 receptor expressed in EDG3-bla U2OS cells incubated for 18 hrs prior to GenBlazer substrate addition by beta-arrestin recruitment assay Agonist activity at human S1P3 receptor expressed in EDG3-bla U2OS cells incubated for 18 hrs prior to GenBlazer substrate addition by beta-arrestin recruitment assay	[PMID: 26687487]

In Vitro

FTY720 (S)-Phosphate is an agonist of S1PR1. FTY720 (S)-Phosphate (Tys, 1 μM) maintains S1PR1 protein expression, enhances the human pulmonary artery endothelial cells barrier via S1PR1, but shows no effect on inducing ubiquitination of S1PR1. FTY720 (S)-Phosphate (0.01-50 μM) also reduces β-arrestin recruitment to S1PR1^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

In Vivo

FTY720 (S)-Phosphate (0.5 mg/kg, i.p.) preserves S1PR1 expression in mouse lungs, is protective in bleomycin-induced acute lung injury (ALI) and attenuates lung tissue leukocyte infiltration in bleomycin-injured mice^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Chemical Information

CAS No. 402616-26-6
Appearance Solid
Molecular Weight 387.45
Formula C₁₉H₃₄NO₅P
Color White to off-white
SMILES

OC[C@](CCC1=CC=C(CCCCCCCC)C=C1)(N)COP(O)(O)=O
Synonyms

(S)-FTY720P; (S)-FTY720 phosphate
Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years

* The compound is unstable in solutions, freshly prepared is recommended.

Publications (3)

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Journal Impact Factor

Cell Commun Signal

Regulation of CXCR4 function by S1P1 through heteromerization. [Abstract]

2025 Feb 26;23(1):111. PMID: 40012038
Front Immunol

Clostridium butyricum CGMCC0313.1 Protects against Autoimmune Diabetes by Modulating Intestinal Immune Homeostasis and Inducing Pancreatic Regulatory T Cells. [Abstract]

2017 Oct 19:8:1345. PMID: 29097999
Commun Biol

Cell-based and isoform-selective G protein-coupled receptor kinase assays for comprehensive inhibitor evaluation. [Abstract]

2026 Jan 16;9(1):287. PMID: 41545717

Solvent & Solubility

In Vitro:

DMSO : 1 mg/mL (2.58 mM; ultrasonic and warming and heat to 80°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

H₂O : < 0.1 mg/mL (insoluble)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

2.5810 mL

12.9049 mL

25.8098 mL

5 mM

---

10 mM

---

Please refer to the solubility information to select the appropriate solvent. The compound is unstable in solutions, freshly prepared is recommended.

Molarity Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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Dilution Calculator

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In Vivo:

The following protocol is derived from the literature and is for reference only. It is recommended to first try a small sample.

Protocol 1

FTY720 (S)-Phosphate is dissolved in sterile water^[2].

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

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g
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Working solution concentration: 0.22 mg/mL

Protocol

Animal Administration^[1]

Mice^[1] Male C57BL/6 (20-25 g) mice 8-10 weeks old receive a single intratracheal dose of bleomycin at 0.6 U/kg (or sterile saline) on Day 0 followed immediately by intraperitoneal injection of FTY720 (S)-Phosphate (0.5 mg/kg), FTY720 (0.5 mg/kg), or saline. Additional doses of FTY720 (S)-Phosphate or FTY720 are injected on Days 3 and 6. Bronchoalveolar lavage (BAL) fluid and lungs are then collected on Day 7. BAL fluid is used to detect BAL protein levels, WBC count, and WBC differential count. Lungs are perfused with saline to remove blood for Western blot, tissue albumin, and histopathology evaluation. Peripheral blood is obtained on Day 7 for examination of total cell counts and lymphocytes. Experiments are repeated 3 times. 6-10 mice are used per experimental group^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Purity & Documentation

Select Batch:

Purity: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (271 KB)
SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

References

[1]. Wang L, et al. FTY720 (s)-phosphonate preserves sphingosine 1-phosphate receptor 1 expression and exhibits superior barrier protection to FTY720 in acute lung injury. Crit Care Med. 2014 Mar;42(3):e189-99. [Content Brief]

[2]. Lingling Jia, et al. Clostridium butyricum CGMCC0313.1 Protects against Autoimmune Diabetes by Modulating Intestinal Immune Homeostasis and Inducing Pancreatic Regulatory T Cells. Front Immunol. 2017 Oct 19;8:1345. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Please refer to the solubility information to select the appropriate solvent. The compound is unstable in solutions, freshly prepared is recommended.

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
DMSO	1 mM	2.5810 mL	12.9049 mL	25.8098 mL	64.5245 mL

Keywords

FTY720 (S)-Phosphate

3 Cited Publications

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FTY720 (S)-Phosphate

MCE Bioactive Compound Library

Publications Citing Use of MedChemExpress (MCE) FTY720 (S)-Phosphate

All LPL Receptor Isoforms

Biological Activity

Chemical Information

Publications (3)

Solvent & Solubility

Protocol

Purity & Documentation

References

Help & FAQs

Complete Stock Solution Preparation Table

FTY720 (S)-Phosphate

MCE Bioactive Compound Library

Publications Citing Use of MedChemExpress (MCE) FTY720 (S)-Phosphate

All LPL Receptor Isoforms

Biological Activity

Chemical Information

Publications (3)

Solvent & Solubility

Protocol

Purity & Documentation

References

Help & FAQs

Complete Stock Solution Preparation Table

FTY720 (S)-Phosphate Related Classifications

HY-15382 Related Classifications