(S)-FTY720-phosphonate
Based on 1 Customer Validation
FTY720 (S)-Phosphate is an agonist of S1P receptor 1 (S1PR1), used in the research of acute inflammatory diseases such as acute lung injury.
For research use only. We do not sell to patients.
- Purity: 99.91%
- CAS No.: 1142015-10-8
- Formula: C20H36NO4P
- Molecular Weight:385.48
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Storage:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
S1PR1[1]
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Cell Line
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Type | Value | Description | References |
|---|---|---|---|---|
| IEC-6 | EC50 |
75 nM
Compound: Page no 3587, R14C2
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Inhibition of apoptosis in rat IEC-6 cells assessed as decrease in CPT-induced DNA fragmentation
Inhibition of apoptosis in rat IEC-6 cells assessed as decrease in CPT-induced DNA fragmentation
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[PMID: 30409535] |
FTY720 (S)-Phosphate is an agonist of S1PR1. FTY720 (S)-Phosphate (Tys, 1 μM) maintains S1PR1 protein expression, enhances the human pulmonary artery endothelial cells barrier via S1PR1, but shows no effect on inducing ubiquitination of S1PR1. FTY720 (S)-Phosphate (0.01-50 μM) also reduces β-arrestin recruitment to S1PR1[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 1142015-10-8
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Appearance Solid
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Molecular Weight 385.48
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Formula C20H36NO4P
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Color White to off-white
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SMILES
O=P(O)(O)CC[C@@](CO)(N)CCC1=CC=C(C=C1)CCCCCCCC
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Solvent & Solubility
DMSO : 25 mg/mL (64.85 mM; ultrasonic and adjust pH to 2 with 1M HCl; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (6.49 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
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Data Sheet (269 KB)
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SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Portuguese - PT (251 KB)
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Handling Instructions (2659 KB)
References
[1]. Wang L, et al. FTY720 (s)-phosphonate preserves sphingosine 1-phosphate receptor 1 expression and exhibits superior barrier protection to FTY720 in acute lung injury. Crit Care Med. 2014 Mar;42(3):e189-99. [Content Brief]
[2]. Lingling Jia, et al. Clostridium butyricum CGMCC0313.1 Protects against Autoimmune Diabetes by Modulating Intestinal Immune Homeostasis and Inducing Pancreatic Regulatory T Cells. Front Immunol. 2017 Oct 19;8:1345. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
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| DMSO | 1 mM | 2.5942 mL | 12.9708 mL | 25.9417 mL | 64.8542 mL |
| 5 mM | 0.5188 mL | 2.5942 mL | 5.1883 mL | 12.9708 mL | |
| 10 mM | 0.2594 mL | 1.2971 mL | 2.5942 mL | 6.4854 mL | |
| 15 mM | 0.1729 mL | 0.8647 mL | 1.7294 mL | 4.3236 mL | |
| 20 mM | 0.1297 mL | 0.6485 mL | 1.2971 mL | 3.2427 mL | |
| 25 mM | 0.1038 mL | 0.5188 mL | 1.0377 mL | 2.5942 mL | |
| 30 mM | 0.0865 mL | 0.4324 mL | 0.8647 mL | 2.1618 mL | |
| 40 mM | 0.0649 mL | 0.3243 mL | 0.6485 mL | 1.6214 mL | |
| 50 mM | 0.0519 mL | 0.2594 mL | 0.5188 mL | 1.2971 mL | |
| 60 mM | 0.0432 mL | 0.2162 mL | 0.4324 mL | 1.0809 mL |
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