Cat. No.: HY-14428

ICG-001

66 Cited Publications

Customer Review

Based on 66 publication(s) in Google Scholar

ICG-001 is an inhibitor of β-catenin/TCF mediated transcription. ICG-001 works by specifically binding to cyclic AMP response element-binding protein with an IC₅₀ of 3 μM. ICG-001 selectively blocks the β-catenin/CBP interaction without interfering with the β-catenin/p300 interaction.

For research use only. We do not sell to patients.

Purity: 99.86%
CAS No.: 780757-88-2
Formula: C₃₃H₃₂N₄O₄
Molecular Weight:548.63

Storage:

Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 2 years , -20°C, 1 year

ICG-001 Chemical Structure

CAS No.: 780757-88-2

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Use of MCE Products

Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biological Activity
- Description
- IC₅₀ & Target
- Cellular Effect
- In Vitro
- In Vivo
Chemical Information
Publications (66)
Solvent & Solubility
- In Vitro
- In Vivo
Protocol
Purity & Documentation
References
Help & FAQs
Bioz

Biological Activity

Description

IC₅₀ & Target

IC50: 3 μM (CBP)

Cellular Effect

Cell Line	Type	Value	Description	References
A549	GI₅₀	6.1 μM Compound: ICG001	Antiproliferative activity against human A549 cells after 72 hrs by MTT assay Antiproliferative activity against human A549 cells after 72 hrs by MTT assay	[PMID: 24950489]
A549	IC₅₀	54.1 μM Compound: ICG-001	Antiproliferative activity against Wnt-independent human A549 cells assessed as inhibition of cell proliferation incubated for 48 hrs by CCK-8 assay Antiproliferative activity against Wnt-independent human A549 cells assessed as inhibition of cell proliferation incubated for 48 hrs by CCK-8 assay	[PMID: 36630177]
CT26	IC₅₀	18.59 μM Compound: ICG-001	Antiproliferative activity against Wnt-dependent mouse CT26 cells assessed as inhibition of cell proliferation incubated for 48 hrs by CCK-8 assay Antiproliferative activity against Wnt-dependent mouse CT26 cells assessed as inhibition of cell proliferation incubated for 48 hrs by CCK-8 assay	[PMID: 36630177]
HCT-116	IC₅₀	32.71 μM Compound: ICG-001	Antiproliferative activity against Wnt-dependent human HCT-116 cells assessed as inhibition of cell proliferation incubated for 48 hrs by CCK-8 assay Antiproliferative activity against Wnt-dependent human HCT-116 cells assessed as inhibition of cell proliferation incubated for 48 hrs by CCK-8 assay	[PMID: 36630177]
HCT-116	IC₅₀	38 μM Compound: ICG-001	Antiproliferative activity against beta-catenin signalling overexpressing human HCT-116 cells assessed as reduction in cell growth incubated for 72 hrs by MTS assay Antiproliferative activity against beta-catenin signalling overexpressing human HCT-116 cells assessed as reduction in cell growth incubated for 72 hrs by MTS assay	[PMID: 34270257]
HCT-116	IC₅₀	38 μM Compound: ICG-001	Antiproliferative activity against human HCT-116 cells assessed as cell growth inhibition measured after 72 hrs by MTS assay Antiproliferative activity against human HCT-116 cells assessed as cell growth inhibition measured after 72 hrs by MTS assay	[PMID: 34382808]
HEK293	IC₅₀	75.39 μM Compound: ICG-001	Antiproliferative activity against Wnt-independent human HEK293 cells assessed as inhibition of cell proliferation incubated for 48 hrs by CCK-8 assay Antiproliferative activity against Wnt-independent human HEK293 cells assessed as inhibition of cell proliferation incubated for 48 hrs by CCK-8 assay	[PMID: 36630177]
HepG2	GI₅₀	12.7 μM Compound: ICG001	Antiproliferative activity against human HepG2 cells after 72 hrs by MTT assay Antiproliferative activity against human HepG2 cells after 72 hrs by MTT assay	[PMID: 24950489]
HT-29	GI₅₀	17.2 μM Compound: ICG001	Antiproliferative activity against human HT-29 cells after 72 hrs by MTT assay Antiproliferative activity against human HT-29 cells after 72 hrs by MTT assay	[PMID: 24950489]
HT-29	IC₅₀	10.8 μM Compound: ICG-001	Cytotoxicity against human HT-29 cells assessed as cell viability at 1.563 to 50 uM measured after 72 hrs by plate reader method Cytotoxicity against human HT-29 cells assessed as cell viability at 1.563 to 50 uM measured after 72 hrs by plate reader method	[PMID: 34062253]
HT-29	IC₅₀	18.7 μM Compound: ICG001	Inhibition of Wnt signaling in human HT29 cells assessed as inhibition of beta-catenin-mediated Tcf/Lef transcriptional activity after 24 hrs by dual luciferase reporter gene assay relative to control Inhibition of Wnt signaling in human HT29 cells assessed as inhibition of beta-catenin-mediated Tcf/Lef transcriptional activity after 24 hrs by dual luciferase reporter gene assay relative to control	[PMID: 24950489]
LoVo	GI₅₀	15.6 μM Compound: ICG001	Antiproliferative activity against human LoVo cells after 72 hrs by MTT assay Antiproliferative activity against human LoVo cells after 72 hrs by MTT assay	[PMID: 24950489]
MCF-10A	IC₅₀	21 μM Compound: ICG-001	Cytotoxicity against human MCF-10A cells assessed as reduction in cell growth incubated for 72 hrs by MTS assay Cytotoxicity against human MCF-10A cells assessed as reduction in cell growth incubated for 72 hrs by MTS assay	[PMID: 34270257]
MCF-10A	IC₅₀	21 μM Compound: ICG-001	Cytotoxicity against human MCF-10A cells assessed as cell growth inhibition measured after 72 hrs by MTS assay Cytotoxicity against human MCF-10A cells assessed as cell growth inhibition measured after 72 hrs by MTS assay	[PMID: 34382808]
MDA-MB-231	IC₅₀	7.2 μM Compound: ICG-001	Antiproliferative activity against beta-catenin signalling overexpressing human MDA-MB-231 cells assessed as reduction in cell growth incubated for 72 hrs by MTS assay Antiproliferative activity against beta-catenin signalling overexpressing human MDA-MB-231 cells assessed as reduction in cell growth incubated for 72 hrs by MTS assay	[PMID: 34270257]
MDA-MB-231	IC₅₀	7.2 μM Compound: ICG-001	Antiproliferative activity against human MDA-MB-231 cells assessed as cell growth inhibition measured after 72 hrs by MTS assay Antiproliferative activity against human MDA-MB-231 cells assessed as cell growth inhibition measured after 72 hrs by MTS assay	[PMID: 34382808]
MDA-MB-468	IC₅₀	31 μM Compound: ICG-001	Antiproliferative activity against beta-catenin signalling overexpressing human MDA-MB-468 cells assessed as reduction in cell growth incubated for 72 hrs by MTS assay Antiproliferative activity against beta-catenin signalling overexpressing human MDA-MB-468 cells assessed as reduction in cell growth incubated for 72 hrs by MTS assay	[PMID: 34270257]
MDA-MB-468	IC₅₀	31 μM Compound: ICG-001	Antiproliferative activity against human MDA-MB-468 cells assessed as cell growth inhibition measured after 72 hrs by MTS assay Antiproliferative activity against human MDA-MB-468 cells assessed as cell growth inhibition measured after 72 hrs by MTS assay	[PMID: 34382808]
RKO	IC₅₀	77.48 μM Compound: ICG-001	Antiproliferative activity against Wnt-independent human RKO cells assessed as inhibition of cell proliferation incubated for 48 hrs by CCK-8 assay Antiproliferative activity against Wnt-independent human RKO cells assessed as inhibition of cell proliferation incubated for 48 hrs by CCK-8 assay	[PMID: 36630177]
SW480	IC₅₀	1.3 μM Compound: ICG-001	Inhibition of CBP binding to beta-casein in human SW480 cells by immunoblot analysis Inhibition of CBP binding to beta-casein in human SW480 cells by immunoblot analysis	[PMID: 23232060]
SW480	IC₅₀	6.1 μM Compound: ICG-001	Antiproliferative activity against beta-catenin signalling overexpressing human SW480 cells assessed as reduction in cell growth incubated for 72 hrs by MTS assay Antiproliferative activity against beta-catenin signalling overexpressing human SW480 cells assessed as reduction in cell growth incubated for 72 hrs by MTS assay	[PMID: 34270257]
SW480	IC₅₀	6.1 μM Compound: ICG-001	Antiproliferative activity against human SW480 cells assessed as cell growth inhibition measured after 72 hrs by MTS assay Antiproliferative activity against human SW480 cells assessed as cell growth inhibition measured after 72 hrs by MTS assay	[PMID: 34382808]
SW480	IC₅₀	8.43 μM Compound: ICG-001	Antiproliferative activity against Wnt-dependent human SW480 cells assessed as inhibition of cell proliferation incubated for 48 hrs by CCK-8 assay Antiproliferative activity against Wnt-dependent human SW480 cells assessed as inhibition of cell proliferation incubated for 48 hrs by CCK-8 assay	[PMID: 36630177]

In Vitro

ICG-001 (5μM) inhibits leptin-induced EMT, invasion and tumorsphere formation in MCF7 cells^[1]. ICG-001 can phenotypically rescue normal nerve growth factor (NGF)-induced neuronal differentiation and neurite outgrowth in the presenilin-1 mutant cells, emphasizing the importance of the TCF/β-catenin signaling pathway on neurite outgrowth and neuronal differentiation^[2]. ICG-001 (25μM) treatment reduces the steady-state levels of Survivin and Cyclin D1 RNA and protein in SW480 cells, both of which can be up-regulated by β-catenin. ICG-001 selectively induces apoptosis in transformed cells but not in normal colon cells, and reduces in vitro growth of colon carcinoma cells^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

In Vivo

ICG-001 (5 mg/kg per day) significantly inhibits beta-catenin signaling in mice, while concurrently preserving the epithelium^[2]. Administration of a water-soluble analog of ICG-001 for 9 weeks reduces the formation of colon and small intestinal polyps by 42% as effectively as the nonsteroidal antiinflammatory agent MK-231, which has consistently demonstrated efficacy in this model. ICG-001 (150 mg/kg, i.v.) demonstrates a dramatic reduction in tumor volume over the 19-day course of treatment, with no mortality or weight loss in the SW620 nude mouse xenograft model of tumor regression^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Chemical Information

CAS No. 780757-88-2
Appearance Solid
Molecular Weight 548.63
Formula C₃₃H₃₂N₄O₄
Color Off-white to light yellow
SMILES

O=C(NCC1=CC=CC=C1)N2CCC(N([C@@]2(CN(C3=O)CC4=C5C=CC=CC5=CC=C4)[H])[C@H]3CC6=CC=C(C=C6)O)=O
Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	2 years
	-20°C	1 year

Publications (66)

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Signal Transduct Target Ther

Oxidative stress-triggered Wnt signaling perturbation characterizes the tipping point of lung adeno-to-squamous transdifferentiation. [Abstract]

2023 Jan 11;8(1):16. PMID: 36627278
Cancer Commun (Lond)

N6-methyladenosine reader hnRNPA2B1 recognizes and stabilizes NEAT1 to confer chemoresistance in gastric cancer. [Abstract]

2024 Apr;44(4):469-490. PMID: 38512764
Mol Cell

mTOR inhibition reprograms cellular lipid homeostasis by inducing alternative lipid uptake and promoting cholesterol transport. [Abstract]

2025 Sep 18;85(18):3486-3504.e7. PMID: 40972529
Cancer Res

Ephrin A1 Stimulates CCL2 Secretion to Facilitate Premetastatic Niche Formation and Promote Gastric Cancer Liver Metastasis. [Abstract]

2025 Jan 15;85(2):263-276. PMID: 39412948
Cancer Res

NOTCH Signaling via WNT Regulates the Proliferation of Alternative, CCR2-Independent Tumor-Associated Macrophages in Hepatocellular Carcinoma. [Abstract]

2019 Aug 15;79(16):4160-4172. PMID: 31266773
Sci Immunol

Macrophages restrict tumor immune infiltration by controlling collagen topography. [Abstract]

2026 Mar 20;11(117):eadw8291. PMID: 41860994
Nat Commun

Stromal androgen signaling acts as tumor niches to drive prostatic basal epithelial progenitor-initiated oncogenesis. [Abstract]

2022 Nov 2;13(1):6552. PMID: 36323713
Nat Commun

Aberrant androgen action in prostatic progenitor cells induces oncogenesis and tumor development through IGF1 and Wnt axes. [Abstract]

2022 Jul 28;13(1):4364. PMID: 35902588
Acta Pharm Sin B

Transferrin receptor-targeted immunostimulant for photodynamic immunotherapy against metastatic tumors through β-catenin/CREB interruption. [Abstract]

2024 Sep;14(9):4118-4133. PMID: 39309507
Acta Pharm Sin B

WSB1 regulates c-Myc expression through β-catenin signaling and forms a feedforward circuit. [Abstract]

2022 Mar;12(3):1225-1239. PMID: 35530152
J Exp Clin Cancer Res

ICG-001 suppresses growth of gastric cancer cells and reduces chemoresistance of cancer stem cell-like population. [Abstract]

2017 Sep 11;36(1):125. PMID: 28893318

ICG-001 purchased from MedChemExpress. Usage Cited in: J Exp Clin Cancer Res. 2017 Sep 11;36(1):125. [Abstract]

ICG-001 mediates the association of β-catenin with CBP, P300, E-cadherin and N-cadherin. a and b Western blotting analysis of Wnt related proteins from total proteins and extracted nuclear and cytoplasmic proteins.
Nat Cardiovasc Res

2024 Apr 25:1-7.
MedComm

Attenuation of amyloid-β-induced mitochondrial dysfunction by active components of anthocyanins in HT22 neuronal cells. [Abstract]

2023 Jun 19;4(4):e301. PMID: 37346934
Cancer Lett

Exploring epigenetic dynamics unveils a super-enhancer-mediated NDRG1-β-catenin axis in modulating gemcitabine resistance in pancreatic cancer. [Abstract]

2024 Nov 28:605:217284. PMID: 39366545
Int J Biol Sci

m6A RNA methylation drives kidney fibrosis by upregulating β-catenin signaling. [Abstract]

2024 Jun 3;20(8):3185-3200. PMID: 38904026
Cell Death Dis

Tumor-derived apolipoprotein E confers resistance to temozolomide in pancreatic neuroendocrine tumors. [Abstract]

2025 Dec 13. PMID: 41390817
Cell Death Dis

Crosstalk between hepatic tumor cells and macrophages via Wnt/β-catenin signaling promotes M2-like macrophage polarization and reinforces tumor malignant behaviors. [Abstract]

2018 Jul 18;9(8):793. PMID: 30022048

ICG-001 purchased from MedChemExpress. Usage Cited in: Cell Death Dis. 2018 Jul 18;9(8):793. [Abstract]

BMDMs are cultured with the conditional medium (CM) that is collected from cultured Hepa1-6 cells for 24 h. Meanwhile BMDMs are treated with or without ICG001. The M2-related markers and downstream genes of Wnt signaling are detected by western blotting.
ACS Appl Mater Interfaces

Eu-Doped TiO2 Coatings via One-Step In Situ Preparation Enhance Macrophage Polarization and Osseointegration of Implants. [Abstract]

2025 Feb 12;17(6):8886-8900. PMID: 39885805
ACS Appl Mater Interfaces

Wnt3a-Modified Nanofiber Scaffolds Facilitate Tendon Healing by Driving Macrophage Polarization during Repair. [Abstract]

2023 Feb 22;15(7):9010-9023. PMID: 36758166
Chemosphere

PFDA promotes cancer metastasis through macrophage M2 polarization mediated by Wnt/β-catenin signaling. [Abstract]

2024 Jul 3:142758. PMID: 38969224
Neoplasia

Targeting Wnt/β-catenin signaling enhances the efficacy of anti-CD38 immunotherapy in multiple myeloma. [Abstract]

2025 Oct 23:70:101242. PMID: 41135362
Oncogene

CAPRIN1-mediated sequestration of NCOA4 mRNA into stress granules drives sorafenib resistance in hepatocellular carcinoma. [Abstract]

2026 May;45(16):1411-1424. PMID: 41896589
Cell Chem Biol

Aberrant human ClpP activation disturbs mitochondrial proteome homeostasis to suppress pancreatic ductal adenocarcinoma. [Abstract]

2022 Sep 15;29(9):1396-1408.e8. PMID: 35905743
Cell Death Discov

Matrix metalloproteinase-10 promotes kidney fibrosis by transactivating β-catenin signaling. [Abstract]

2025 May 17;11(1):241. PMID: 40382334
Elife

Renal interstitial cells promote nephron regeneration by secreting prostaglandin E2. [Abstract]

2023 Jan 16:12:e81438. PMID: 36645741
J Pineal Res

MTNR1B loss promotes chordoma recurrence by abrogating melatonin-mediated β-catenin signaling repression. [Abstract]

2019 Sep;67(2):e12588. PMID: 31140197
J Agric Food Chem

Proline Alleviates Low-Protein Diet-Induced Adverse Pregnancy Outcomes by Enriching Akkermansia and Activating Placental Wnt/Igf1 Signaling. [Abstract]

2026 Mar 25;74(11):9281-9293. PMID: 41812033
JCI Insight

Insulin-like growth factor 2 mRNA-binding protein 3 promotes kidney injury by regulating β-catenin signaling. [Abstract]

2023 Jan 24;8(2):e162060. PMID: 36520532
Int J Mol Med

Toxoplasma gondii excretory/secretory proteins promotes osteogenic differentiation of bone marrow mesenchymal stem cells via aerobic glycolysis mediated by Wnt/β‑catenin signaling pathway. [Abstract]

2023 Oct;52(4):91. PMID: 37594122
J Ethnopharmacol

YiQi GuBen formula targets EHMT2 and enhances NEDD4L transcription to combat epithelial-mesenchymal transition and airway remodeling in asthma. [Abstract]

2026 Mar 25:359:121125. PMID: 41475628
J Ethnopharmacol

Medicinal and edible plant Allium macrostemon Bunge for the treatment of testosterone-induced androgenetic alopecia in mice. [Abstract]

2023 Oct 28:315:116657. PMID: 37244409
Mol Ther Oncolytics

Oncolytic Herpes Simplex Virus and PI3K Inhibitor BKM120 Synergize to Promote Killing of Prostate Cancer Stem-like Cells. [Abstract]

2019 Mar 29:13:58-66. PMID: 31016228
Cells

Targeting Wnt/β-Catenin Signaling Exacerbates Ferroptosis and Increases the Efficacy of Melanoma Immunotherapy via the Regulation of MITF. [Abstract]

2022 Nov 12;11(22):3580. PMID: 36429010
Int J Mol Sci

Functionalized 3D-Printed ST2/Gelatin Methacryloyl/Polcaprolactone Scaffolds for Enhancing Bone Regeneration with Vascularization. [Abstract]

2022 Jul 28;23(15):8347. PMID: 35955478
Int J Mol Sci

Label-Free Quantitative Proteomics Combined with Biological Validation Reveals Activation of Wnt/β-Catenin Pathway Contributing to Trastuzumab Resistance in Gastric Cancer. [Abstract]

2018 Jul 6;19(7). pii: E1981. PMID: 29986466

ICG-001 purchased from MedChemExpress. Usage Cited in: Int J Mol Sci. 2018 Jul 6;19(7). pii: E1981. [Abstract]

Cells exposed to ICG-001 for 24 h under different concentrations of (0, 6, 8 μM) are analyzed by Western blot for full-length PARP and cleaved PARP, with β-actin being a loading control.
Cancer Sci

Wnt-β-catenin signaling regulates ABCC3 (MRP3) transporter expression in colorectal cancer. [Abstract]

2016 Dec;107(12):1776-1784. PMID: 27709738

ICG-001 purchased from MedChemExpress. Usage Cited in: Cancer Sci. 2016 Dec;107(12):1776-1784. [Abstract]

The amount of ABCC3 protein is increased by ICG-001 treatment in HT-29 cells.
J Ovarian Res

TRIB3 regulates Cx43 expression in human granulosa cells via the Wnt/β-catenin signaling pathway: an in vitro study. [Abstract]

2025 Jul 2;18(1):139. PMID: 40605006
Biochim Biophys Acta Mol Basis Dis

Renal tubular S100A7a impairs fatty acid oxidation and exacerbates renal fibrosis via both intracellular and extracellular pathway. [Abstract]

2025 Jan 6:167656. PMID: 39778778
FASEB J

Long non-coding RNA SNHG11 regulates the Wnt/β-catenin signaling pathway through rho/ROCK in trabecular meshwork cells. [Abstract]

2023 Apr;37(4):e22873. PMID: 36929360
J Cell Mol Med

ASPP2 suppresses tumour growth and stemness characteristics in HCC by inhibiting Warburg effect via WNT/β-catenin/HK2 axis. [Abstract]

2023 Mar;27(5):659-671. PMID: 36752127
Diabetol Metab Syndr

Trigonelline reverses high glucose-induced proliferation, fibrosis of mesangial cells via modulation of Wnt signaling pathway. [Abstract]

2022 Feb 9;14(1):28. PMID: 35139912
Sci Rep

PD-L1 mediates lung fibroblast to myofibroblast transition through Smad3 and β-catenin signaling pathways. [Abstract]

2022 Feb 23;12(1):3053. PMID: 35197539
Neurochem Res

PRMT5 Aggravates Parkinson's Disease Progression Through the Inhibition of Neuronal Autophagy Through DKK1. [Abstract]

2025 Dec 11;51(1):12. PMID: 41379372
J Virol

Transmissible Gastroenteritis Virus Infection Promotes the Self-Renewal of Porcine Intestinal Stem Cells via Wnt/β-Catenin Pathway. [Abstract]

2022 Sep 28;96(18):e0096222. PMID: 36073923
Cell Signal

GC-MSCs transcriptionally upregulate SALL4 in gastric cancer through miR-4669/TIMP3/β-catenin signaling. [Abstract]

2025 Jun:130:111668. PMID: 39965736
Am J Pathol

Growth Differentiation Factor 11 Promotes Corneal Endothelial Injury Repair by Activating the SRY-Box Transcription Factor 9-β-Catenin Signaling Pathway. [Abstract]

2026 Mar 26:S0002-9440(26)00073-8. PMID: 41903789
Am J Pathol

TGFβ1-Induced Fibrotic Responses of Conjunctival Fibroblasts through the Wnt/β-Catenin/CRYAB Signaling Pathway. [Abstract]

2024 Jun 13:S0002-9440(24)00201-3. PMID: 38879081
Food Chem Toxicol

Polystyrene microplastics facilitate renal fibrosis through accelerating tubular epithelial cell senescence. [Abstract]

2024 Jul 23:114888. PMID: 39053876
Am J Physiol Renal Physiol

Soluble (pro)renin receptor promotes the fibrotic response in renal proximal tubule epithelial cells in vitro via the Akt/β-catenin/Snail signaling pathway. [Abstract]

2020 Nov 1;319(5):F941-F953. PMID: 32865015
Am J Physiol Renal Physiol

Soluble (pro)renin receptor regulation of ENaC involved in aldosterone signaling in cultured collecting duct cells. [Abstract]

2020 Mar 1;318(3):F817-F825. PMID: 31841392
J Pharm Biomed Anal

Quantitative determination of ICG-001 in rat plasma using HPLC-MS/MS: A pharmacokinetic study. [Abstract]

2022 Sep 20:219:114949. PMID: 35863168
Cell Biol Int

Epithelial cell adhesion molecule promotes breast cancer resistance protein-mediated multidrug resistance in breast cancer by inducing partial epithelial-mesenchymal transition. [Abstract]

2021 Aug;45(8):1644-1653. PMID: 33760350
Exp Biol Med

The β-catenin/CBP signaling axis participates in sepsis-induced inflammatory lung injury. [Abstract]

2022 Sep;247(17):1548-1557. PMID: 35665630
Brain Res

Mesenchymal stem cell-derived exosomes rescue stress-dependent morphological deficits in primary hippocampal neurons via Wnt5a/β-catenin/WAVE2 pathway. [Abstract]

2026 May 15:1879:150207. PMID: 41698632
PLoS One

17β-Estradiol promotes metastasis in triple-negative breast cancer through the Calpain/YAP/β-catenin signaling axis. [Abstract]

2024 Mar 28;19(3):e0298184. PMID: 38547301
PLoS One

Inhibition of RIP1-RIP3-mediated necroptosis attenuates renal fibrosis via Wnt3α/β-catenin/GSK-3β signaling in unilateral ureteral obstruction. [Abstract]

2022 Oct 12;17(10):e0274116. PMID: 36223414
Biochem Biophys Res Commun

MPC1 deficiency promotes melanoma progression via Wnt/β-catenin signaling. [Abstract]

2026 Feb 19:801:153310. PMID: 41576477
bioRxiv

2026 Jun 9.
SSRN

2024 Apr 5.
SSRN

2024 Apr 5.
Research Square Preprint

2023 Aug 21.
bioRxiv

2023 Feb 24.
bioRxiv

2023 Feb 10.
Research Square Preprint

2021 Apr.
Small Ruminants Res

178 (2019) 30-36.
Oncotarget

Insufficient microwave ablation-induced promotion of distant metastasis is suppressed by β-catenin pathway inhibition in breast cancer. [Abstract]

2017 Dec 1;8(70):115089-115101. PMID: 29383144

ICG-001 purchased from MedChemExpress. Usage Cited in: Oncotarget. 2017 Dec 1;8(70):115089-115101. [Abstract]

ICG001 depresses the heat-induced over-expression of EMT markers (N-cadherin, Snail), and partially recovers the expression of E-cadherin in heat-treated SUM-1315 and ZR-75-1 cells.

ICG-001 purchased from MedChemExpress. Usage Cited in: Oncotarget. 2017 Dec 1;8(70):115089-115101. [Abstract]

ICG001 normalizes the down expression of E-cadherin and reduces the over-expression of N-cadherin and snail in residual tumor tissue after insufficient microwave ablation (MWA).

Solvent & Solubility

In Vitro:

DMSO : ≥ 50 mg/mL (91.14 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

* "≥" means soluble, but saturation unknown.

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

1.8227 mL

9.1136 mL

18.2272 mL

5 mM

0.3645 mL

1.8227 mL

3.6454 mL

10 mM

0.1823 mL

0.9114 mL

1.8227 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Molarity Calculator

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Dilution Calculator

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In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

Stock Solution Working Solution

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline

Solubility: ≥ 2.5 mg/mL (4.56 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)

Solubility: 2.5 mg/mL (4.56 mM); Suspended solution; Need ultrasonic and warming

This protocol yields a suspended solution of 2.5 mg/mL. Suspended solution can be used for oral and intraperitoneal injection.

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil

Solubility: ≥ 2.5 mg/mL (4.56 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

Working Solution

For the following dissolution methods, please prepare the working solution directly:

It is recommended to prepare fresh solutions and use them promptly within a short period of time.

The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 15% Cremophor EL 85% Saline

Solubility: 1.67 mg/mL (3.04 mM); Suspended solution; Need ultrasonic

Protocol 2

Add each solvent one by one: 17% Solutol HS-15 in Saline

Solubility: 1.67 mg/mL (3.04 mM); Suspended solution; Need ultrasonic

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

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mg/kg
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g
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

%
DMSO +

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
%
+
+
%
Tween-80 + +
%
Saline +

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Working solution concentration: 0.22 mg/mL

Protocol

Cell Assay^[2]

To evaluate effects of ICG-001 on α-SMA and collagen type 1 expression, RLE-6TN cells are treated with TGF-β1 (0.25 ng/mL) in the presence or absence of ICG-001 (5.0 μM). After 24 h, cells are harvested and mRNA isolated for analysis by qPCR. RNA is reverse-transcribed using SuperScript reverse transcriptase. Quantitative PCR is performed with SYBR-Green PCR using Real-Time PCR System HT7900. The amplification protocol is set as follows: 95°C denaturation for 10 min followed by 40 cycles of 15-s denaturation at 95°C, 1 min of annealing/extension, and data collection at 60°C.

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Administration^[3]

Seven-week-old male C57BL/6J-Apc^Min/+and WT C57BL/6J mice are treated orally for 9 weeks with ICG-001a (300 mg/kg per day) or vehicle (1% carboxymethylcellulose), once daily, six times per week. MK-231 is administered in drinking water (160 ppm, dissolved in 8 mM Na₂PO₄ buffer, pH 7.6). At 16 weeks, the polyp number is counted manually by using a dissecting microscope.

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Purity & Documentation

Select Batch:

Purity: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (286 KB)
SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Portuguese - PT (393 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

References

[1]. Yan D, et al, Leptin-induced epithelial-mesenchymal transition in breast cancer cells requires β-catenin activation via Akt/GSK3- and MTA1/Wnt1 protein-dependent pathways. J Biol Chem, 2012, 287(11), 8598-8612. [Content Brief]

[2]. Henderson WR Jr, et al, Inhibition of Wnt/beta-catenin/CREB binding protein (CBP) signaling reverses pulmonary fibrosis. Proc Natl Acad Sci USA, 2010, 107(32), 14309-14314. [Content Brief]

[3]. Emami KH, et al. A small molecule inhibitor of beta-catenin/CREB-binding protein transcription [corrected]. Proc Natl Acad Sci USA, 2004, 101(34), 12682-12687. [Content Brief]

[4]. Liu Y, et al. ICG-001 suppresses growth of gastric cancer cells and reduces chemoresistance of cancer stem cell-like population. J Exp Clin Cancer Res. 2017 Sep 11;36(1):125. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
DMSO	1 mM	1.8227 mL	9.1136 mL	18.2272 mL	45.5681 mL
	5 mM	0.3645 mL	1.8227 mL	3.6454 mL	9.1136 mL
	10 mM	0.1823 mL	0.9114 mL	1.8227 mL	4.5568 mL
	15 mM	0.1215 mL	0.6076 mL	1.2151 mL	3.0379 mL
	20 mM	0.0911 mL	0.4557 mL	0.9114 mL	2.2784 mL
	25 mM	0.0729 mL	0.3645 mL	0.7291 mL	1.8227 mL
	30 mM	0.0608 mL	0.3038 mL	0.6076 mL	1.5189 mL
	40 mM	0.0456 mL	0.2278 mL	0.4557 mL	1.1392 mL
	50 mM	0.0365 mL	0.1823 mL	0.3645 mL	0.9114 mL
	60 mM	0.0304 mL	0.1519 mL	0.3038 mL	0.7595 mL
	80 mM	0.0228 mL	0.1139 mL	0.2278 mL	0.5696 mL

Keywords

Product Name:
Cat. No.:
Quantity:
MCE Japan Authorized Agent:

Product Name:
CAS No.:
Species:
Purity:
Quantity:

ICG-001

Apoptosis Compound Library

Immunology/Inflammation Compound Library

Stem Cell Signaling Compound Library

Wnt/Hedgehog/Notch Compound Library

Anti-Cancer Compound Library

Anti-Aging Compound Library

Differentiation Inducing Compound Library

Anti-Cardiovascular Disease Compound Library

Cytoskeleton Compound Library

Neuroprotective Compound Library

Anti-Breast Cancer Compound Library

Transcription Factor-Targeted Library

Anti-Liver Cancer Compound Library

Anti-Colorectal Cancer Compound Library

Protein-protein Interaction Inhibitor Library

Anti-Prostate Cancer Compound Library

Anti-Pulmonary Fibrosis Compound Library

Osteogenesis Compound Library

Cancer Stem Cells Compound Library

Antihypertensive Compound Library

Highly Selective Inhibitors Library

Highly Selective Activators Library

Cell Death Library

Extracellular Vesicles (EVs) Compound Library

Anti-Ovarian Cancer Compound Library

Bioactive Compound Library Max

Anti-Aging Compound Library Mini

MCE Bioactive Compound Library

Bioactive Compound Library

Anti-Gastric Cancer Compound Library

Anti-Fibrosis Compound Library

Non-Alcoholic Fatty Liver Disease (NAFLD) Compound Library

Cell Proliferation Compound Library

High-Throughput Bioactive Compound Library

RNA Binding Bioactive Compound Library

Publications Citing Use of MedChemExpress (MCE) ICG-001

Biological Activity

Chemical Information

Publications (66)

Solvent & Solubility

Protocol

Purity & Documentation

References

Help & FAQs

Complete Stock Solution Preparation Table

ICG-001 Related Classifications

Cat. No.: HY-14428