1. Stem Cell/Wnt Apoptosis
  2. β-catenin Apoptosis
  3. ICG-001

ICG-001 

Cat. No.: HY-14428 Purity: 99.86%
COA Handling Instructions

ICG-001 is an inhibitor of β-catenin/TCF mediated transcription. ICG-001 works by specifically binding to cyclic AMP response element-binding protein with an IC50 of 3 μM. ICG-001 selectively blocks the β-catenin/CBP interaction without interfering with the β-catenin/p300 interaction.

For research use only. We do not sell to patients.

ICG-001 Chemical Structure

ICG-001 Chemical Structure

CAS No. : 780757-88-2

Size Price Stock Quantity
Solid + Solvent
10 mM * 1 mL in DMSO
ready for reconstitution
USD 96 In-stock
Solution
10 mM * 1 mL in DMSO USD 96 In-stock
Solid
5 mg USD 79 In-stock
10 mg USD 132 In-stock
50 mg USD 462 In-stock
100 mg USD 726 In-stock
200 mg   Get quote  
500 mg   Get quote  

* Please select Quantity before adding items.

This product is a controlled substance and not for sale in your territory.

Customer Review

Based on 43 publication(s) in Google Scholar

Top Publications Citing Use of Products

37 Publications Citing Use of MCE ICG-001

WB
IHC

    ICG-001 purchased from MedChemExpress. Usage Cited in: Cell Death Dis. 2018 Jul 18;9(8):793.  [Abstract]

    BMDMs are cultured with the conditional medium (CM) that is collected from cultured Hepa1-6 cells for 24 h. Meanwhile BMDMs are treated with or without ICG001. The M2-related markers and downstream genes of Wnt signaling are detected by western blotting.

    ICG-001 purchased from MedChemExpress. Usage Cited in: Int J Mol Sci. 2018 Jul 6;19(7). pii: E1981.  [Abstract]

    Cells exposed to ICG-001 for 24 h under different concentrations of (0, 6, 8 μM) are analyzed by Western blot for full-length PARP and cleaved PARP, with β-actin being a loading control.

    ICG-001 purchased from MedChemExpress. Usage Cited in: J Exp Clin Cancer Res. 2017 Sep 11;36(1):125.  [Abstract]

    ICG-001 mediates the association of β-catenin with CBP, P300, E-cadherin and N-cadherin. a and b Western blotting analysis of Wnt related proteins from total proteins and extracted nuclear and cytoplasmic proteins.

    ICG-001 purchased from MedChemExpress. Usage Cited in: Oncotarget. 2017 Dec 1;8(70):115089-115101.  [Abstract]

    ICG001 normalizes the down expression of E-cadherin and reduces the over-expression of N-cadherin and snail in residual tumor tissue after insufficient microwave ablation (MWA).

    ICG-001 purchased from MedChemExpress. Usage Cited in: Oncotarget. 2017 Dec 1;8(70):115089-115101.  [Abstract]

    ICG001 depresses the heat-induced over-expression of EMT markers (N-cadherin, Snail), and partially recovers the expression of E-cadherin in heat-treated SUM-1315 and ZR-75-1 cells.

    ICG-001 purchased from MedChemExpress. Usage Cited in: Cancer Sci. 2016 Dec;107(12):1776-1784.  [Abstract]

    The amount of ABCC3 protein is increased by ICG-001 treatment in HT-29 cells.
    • Biological Activity

    • Protocol

    • Purity & Documentation

    • References

    • Customer Review

    Description

    ICG-001 is an inhibitor of β-catenin/TCF mediated transcription. ICG-001 works by specifically binding to cyclic AMP response element-binding protein with an IC50 of 3 μM. ICG-001 selectively blocks the β-catenin/CBP interaction without interfering with the β-catenin/p300 interaction.

    IC50 & Target

    IC50: 3 μM (CBP)

    In Vitro

    ICG-001 (5μM) inhibits leptin-induced EMT, invasion and tumorsphere formation in MCF7 cells[1].?ICG-001 can phenotypically rescue normal nerve growth factor (NGF)-induced neuronal differentiation and neurite outgrowth in the presenilin-1 mutant cells, emphasizing the importance of the TCF/β-catenin signaling pathway on neurite outgrowth and neuronal differentiation[2]. ICG-001 (25μM) treatment reduces the steady-state levels of Survivin and Cyclin D1 RNA and protein in SW480 cells, both of which can be up-regulated by β-catenin. ICG-001 selectively induces apoptosis in transformed cells but not in normal colon cells, and reduces in vitro growth of colon carcinoma cells[3].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    In Vivo

    ICG-001 (5 mg/kg per day) significantly inhibits beta-catenin signaling in mice, while concurrently preserving the epithelium[2].?Administration of a water-soluble analog of ICG-001 for 9 weeks reduces the formation of colon and small intestinal polyps by 42% as effectively as the nonsteroidal antiinflammatory agent MK-231, which has consistently demonstrated efficacy in this model. ICG-001 (150 mg/kg, i.v.) demonstrates a dramatic reduction in tumor volume over the 19-day course of treatment, with no mortality or weight loss in the SW620 nude mouse xenograft model of tumor regression[3].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    Molecular Weight

    548.63

    Formula

    C33H32N4O4

    CAS No.
    Appearance

    Solid

    Color

    White to off-white

    SMILES

    O=C(NCC1=CC=CC=C1)N2CCC(N([C@@]2(CN(C3=O)CC4=C5C=CC=CC5=CC=C4)[H])[C@H]3CC6=CC=C(C=C6)O)=O

    Shipping

    Room temperature in continental US; may vary elsewhere.

    Storage
    Powder -20°C 3 years
    4°C 2 years
    In solvent -80°C 2 years
    -20°C 1 year
    Solvent & Solubility
    In Vitro: 

    DMSO : ≥ 50 mg/mL (91.14 mM)

    *"≥" means soluble, but saturation unknown.

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 1.8227 mL 9.1136 mL 18.2272 mL
    5 mM 0.3645 mL 1.8227 mL 3.6454 mL
    10 mM 0.1823 mL 0.9114 mL 1.8227 mL
    *Please refer to the solubility information to select the appropriate solvent.
    In Vivo:
    • 1.

      Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

      Solubility: ≥ 2.5 mg/mL (4.56 mM); Clear solution

    • 2.

      Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

      Solubility: 2.5 mg/mL (4.56 mM); Suspended solution; Need ultrasonic and warming

    • 3.

      Add each solvent one by one:  10% DMSO    90% Corn Oil

      Solubility: ≥ 2.5 mg/mL (4.56 mM); Clear solution

    • 4.

      Add each solvent one by one:  15% Cremophor EL    85% Saline

      Solubility: 1.67 mg/mL (3.04 mM); Suspended solution; Need ultrasonic

    • 5.

      Add each solvent one by one:  17% Polyethylene glycol 12-hydroxystearate in Saline

      Solubility: 1.67 mg/mL (3.04 mM); Suspended solution; Need ultrasonic

    *All of the co-solvents are available by MedChemExpress (MCE).
    Purity & Documentation

    Purity: 99.86%

    References
    Cell Assay
    [2]

    To evaluate effects of ICG-001 on α-SMA and collagen type 1 expression, RLE-6TN cells are treated with TGF-β1 (0.25 ng/mL) in the presence or absence of ICG-001 (5.0 μM). After 24 h, cells are harvested and mRNA isolated for analysis by qPCR. RNA is reverse-transcribed using SuperScript reverse transcriptase. Quantitative PCR is performed with SYBR-Green PCR using Real-Time PCR System HT7900. The amplification protocol is set as follows: 95°C denaturation for 10 min followed by 40 cycles of 15-s denaturation at 95°C, 1 min of annealing/extension, and data collection at 60°C.

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.

    Animal Administration
    [3]

    Seven-week-old male C57BL/6J-ApcMin/+and WT C57BL/6J mice are treated orally for 9 weeks with ICG-001a (300 mg/kg per day) or vehicle (1% carboxymethylcellulose), once daily, six times per week. MK-231 is administered in drinking water (160 ppm, dissolved in 8 mM Na2PO4 buffer, pH 7.6). At 16 weeks, the polyp number is counted manually by using a dissecting microscope.

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.

    References
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    ICG-001 Related Classifications

    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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    Product Name:
    ICG-001
    Cat. No.:
    HY-14428
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