1. Apoptosis Autophagy
  2. c-Myc Autophagy
  3. KJ Pyr 9

KJ Pyr 9 

Cat. No.: HY-19735 Purity: 99.29%
COA Handling Instructions

KJ Pyr 9 is an inhibitor of MYC with a Kd of 6.5 nM in in vitro assay.

For research use only. We do not sell to patients.

KJ Pyr 9 Chemical Structure

KJ Pyr 9 Chemical Structure

CAS No. : 581073-80-5

Size Price Stock Quantity
Solid + Solvent
10 mM * 1 mL in DMSO
ready for reconstitution
USD 73 In-stock
10 mM * 1 mL in DMSO USD 73 In-stock
5 mg USD 66 In-stock
10 mg USD 92 In-stock
50 mg USD 356 In-stock
100 mg USD 647 In-stock
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Customer Review

Based on 4 publication(s) in Google Scholar

Top Publications Citing Use of Products
  • Biological Activity

  • Protocol

  • Purity & Documentation

  • References

  • Customer Review


KJ Pyr 9 is an inhibitor of MYC with a Kd of 6.5 nM in in vitro assay.

IC50 & Target

Kd: 6.5±1.0 nM (MYC)[1]

In Vitro

KJ Pyr 9 (KJ-Pyr-9) interferes with MYC-MAX complex formation in the cell in a protein fragment complementation assay[1].
KJ Pyr 9 specifically inhibits MYC-induced oncogenic transformation in cell culture and has no or only weak effects on the oncogenic activity of several unrelated oncoproteins[1].
KJ Pyr 9 preferentially interferes with the proliferation of MYC-overexpressing human and avian cells and specifically reduces the MYC-driven transcriptional signature[1].
KJ Pyr 9 against three cell lines (NCI-H460, MDA-MB-231, and SUM-159PT) is tested known to be dependent on increased MYC activity[1].
KJ Pyr 9 can inhibit the proliferation of all cell lines with IC50 values between 5 and 10 μM[1].
KJ Pyr 9 is more sensitive (IC50 values between 1 and 2.5 μM) for the proliferation of Burkitt lymphoma cell lines with constitutively high expression of c-MYC[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

In Vivo

KJ Pyr 9 (i.p.; 10 mg/kg; daily; for 31 d) has the ability to halt tumor growth[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight









Room temperature in continental US; may vary elsewhere.

Powder -20°C 3 years
4°C 2 years
In solvent -80°C 2 years
-20°C 1 year
Solvent & Solubility
In Vitro: 

DMSO : 125 mg/mL (324.36 mM; Need ultrasonic)

Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.5949 mL 12.9745 mL 25.9491 mL
5 mM 0.5190 mL 2.5949 mL 5.1898 mL
10 mM 0.2595 mL 1.2975 mL 2.5949 mL
*Please refer to the solubility information to select the appropriate solvent.
In Vivo:
  • 1.

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

    Solubility: 2.5 mg/mL (6.49 mM); Suspended solution; Need ultrasonic

  • 2.

    Add each solvent one by one:  10% DMSO    90% Corn Oil

    Solubility: ≥ 2.5 mg/mL (6.49 mM); Clear solution

  • 3.

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

    Solubility: 2.08 mg/mL (5.40 mM); Suspended solution; Need ultrasonic

*All of the co-solvents are available by MedChemExpress (MCE).
Purity & Documentation

Purity: 99.29%

Cell Assay

Assays used staining with the redox dye resazurin to measure cell viability. Cells are seeded at 103 per 100 μL well in 96-well plates and grown in the presence of 2.5% FBS. MDA-MB-231 cells are cultured in DMEM; SUM-159PT cells are cultured in HAM’s F12; and NCI-H460 cells are cultured in RPMI-1640. MDA-MB-231 cells are exposed to KJ Pyr 9 (KJ-Pyr-9) for 216 h with fresh compound-containing medium supplied at 120 and 192 h; SUM-159PT cells are exposed to the compound for 120 h and fresh medium with the appropriate compound concentrations is supplied at 48 h; and NCI-H460 cells are grown with compound for 72 h. Triplicate cultures of P493-6 cells are grown in six-well plates from a starting density of 1×105 cells per mL in 4 mL culture medium per well. Compounds are added immediately following cell seeding. Following compound addition, cells are distributed by vortexing the plate at 400 rpm for 10 s. One hundred-microliter samples are taken after vortexing and counted using a Beckman Coulter Z1 counter at 0, 12, 24, 36, 48, 60, 72, and 96 h of incubation[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Administration

Ten 8-wk-old female nude mice (HSD:athymic nude-Foxn1nu) are injected with 5×106 MDA-MB-231 cells s.c. into the left and right flanks. Cells are suspended in high-concentration Matrigel before injection. Xenograft tumors are allowed to grow until the average volume of the tumors reached 100 mm3, as measured by external calipers. At this point, the mice are divided into two groups. One receive 10 mg/kg KJ Pyr 9 and the other receive vehicle only, dosed daily by i.p. injection. Tumor volume and mouse weight are measured daily. Vehicle used in all cases is 10:10:80 Tween 80:DMSO:5% dextrose in water. The mice are treated for a period of 31 d. At the end of the experiment, the mice are euthanized and tumors are excised. Tumors are weighed. Samples of each tumor are fixed in formalin for histology and frozen for Western blotting.

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

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KJ Pyr 9 Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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