1. Immunology/Inflammation
  2. STING

ML RR-S2 CDA ammonium salt (Synonyms: STING-Inducer-1 ammonium salt)

Cat. No.: HY-12885B Purity: 99.23%
Handling Instructions

ML RR-S2 CDA ammonium salt is an inducer of STING (stimulator of interferon genes). ML RR-S2 CDA has enhanced binding affinity to STING and activate all known human STING alleles.

For research use only. We do not sell to patients.

ML RR-S2 CDA ammonium salt Chemical Structure

ML RR-S2 CDA ammonium salt Chemical Structure

CAS No. : 1638750-96-5

Size Price Stock Quantity
10 mM * 1 mL in DMSO USD 2296 In-stock
Estimated Time of Arrival: December 31
1 mg USD 540 In-stock
Estimated Time of Arrival: December 31
5 mg USD 1440 In-stock
Estimated Time of Arrival: December 31
10 mg USD 2400 In-stock
Estimated Time of Arrival: December 31
25 mg USD 4320 In-stock
Estimated Time of Arrival: December 31
50 mg USD 7560 In-stock
Estimated Time of Arrival: December 31
100 mg   Get quote  
200 mg   Get quote  

* Please select Quantity before adding items.

Customer Review

Other Forms of ML RR-S2 CDA ammonium salt:

Featured Recommendations

  • Biological Activity

  • Protocol

  • Technical Information

  • Purity & Documentation

  • References

Description

ML RR-S2 CDA ammonium salt is an inducer of STING (stimulator of interferon genes). ML RR-S2 CDA has enhanced binding affinity to STING and activate all known human STING alleles.

IC50 & Target

STING[1]

In Vitro

ML RR-S2 CDA shows enhanced type I IFN production over CDA in THP-1 human monocytes. In contrast, the dithio, mixed-linkage cyclic dinucleotide (CDN) derivatives (ML RR-CDA, ML RR-S2 CDG, and ML RR-S2 cGAMP) potently activate all five hSTING alleles, including the refractory hSTINGREF and hSTINGQ alleles. ML RR-S2 CDA induces the highest expression of IFN-β and the pro-inflammatory cytokines TNF-α, IL-6, and MCP-1 on a molar equivalent basis, as compared to endogenous ML cGAMP and the TLR3 agonist poly I:C. ML RR-S2 CDA is also found to induce aggregation of STING and induce phosphorylation of TBK1 and IRF3 in mouse bone marrow macrophage (BMM). ML RR-S2 CDA induces significantly higher levels of IFN-α when compared to ML cGAMP[1].

In Vivo

ML RR-S2 CDA shows higher anti-tumor control than the endogenous ML cGAMP. A dose response of the ML RR-S2 CDA compound is performed in B16 tumor-bearing mice, which identifies an optimal antitumor dose level that also elicites maximum tumor antigen-specific CD8+ T cell responses, and improves long-term survival to 50%[1].

Solvent & Solubility
In Vitro: 

DMSO : 15 mg/mL (20.70 mM; Need ultrasonic and warming)

H2O : 12.5 mg/mL (17.25 mM; Need ultrasonic and warming)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 1.3801 mL 6.9004 mL 13.8007 mL
5 mM 0.2760 mL 1.3801 mL 2.7601 mL
10 mM 0.1380 mL 0.6900 mL 1.3801 mL
*Please refer to the solubility information to select the appropriate solvent.
References
Cell Assay
[1]

Cryopreserved hPBMCs are thawed and 1×106 cells per well are plated in a 96 well plate in RPMI media supplemented with 10% FBS, 1% non-essential amino acids, 1% penicillin/streptomycin, L-glutamine, 10 mM HEPES buffer, 1 mM Sodium Pyruvate, 0.055 mM β-ME at 37°C with 5% CO2. Cells are stimulated with 10 μM ML RR-S2 CDA or ML cGAMP for 6 hours and supernatants are harvested. Supernatants are diluted 1:2 and assayed for IFN-α protein using Cytometric Bead Array (CBA) Human Flex Set. Data is collected using a FACSVerse cytometer and analyzed by FCAP Array Software[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Administration
[1]

Mice[1]
WT C57BL/6 mice are inoculated with 5×104 B16.F10 cells in the left flank (n=8). When tumor volumes are 100 mm3 mice receive three IT doses of either ML RR-S2 CDG (25 μg), ML RR-S2 CDA (50 μg), or HBSS as control. WT C57BL/6 mice are inoculated with 5×104 B16.F10 cells in the left flank (n=5). When tumor volumes are 100 mm3 they received three IT doses of ML RR-S2 CDA at 5, 25, 50 or 100 μg or HBSS as control. WT C57BL/6 mice are inoculated with 5×104 B16.F10 cells in the left flank (n=8). When tumor volumes are 100 mm3 they receive three IT doses of 100 μg ML RR-S2 CDA or HBSS as control. Treatments are administered on days 13, 17 and 20 and tumor measurements are taken twice weekly. Results are shown as percent survival by Log-rank (Mantel-Cox) test (A and C).

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

References
Molecular Weight

724.60

Formula

C₂₀H₃₀N₁₂O₁₀P₂S₂

CAS No.

1638750-96-5

SMILES

OC1([H])[[email protected]](O[[email protected]]([S-])(OC[[email protected]](O[[email protected]@H](N2C3=NC=NC(N)=C3N=C2)[[email protected]@H]4O)([H])[[email protected]@]4([H])O5)=O)([H])[[email protected]](N6C7=NC=NC(N)=C7N=C6)O[[email protected]]1([H])CO[[email protected]]5([S-])=O.[NH4+].[NH4+]

Storage
Powder -20°C 3 years
  4°C 2 years
In solvent -80°C 6 months
  -20°C 1 month
Shipping

Room temperature in continental US; may vary elsewhere

Purity: 99.23%

  • Molarity Calculator

  • Dilution Calculator

The molarity calculator equation

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass   Concentration   Volume   Molecular Weight *
= × ×

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

Inquiry Online

Your information is safe with us. * Required Fields.

Product name

 

Salutation

Applicant name *

 

Email address *

Phone number *

 

Organization name *

Country or Region *

 

Requested quantity *

Remarks

Bulk Inquiry

Inquiry Information

Product Name:
ML RR-S2 CDA ammonium salt
Cat. No.:
HY-12885B
Quantity:

ML RR-S2 CDA ammonium salt

Cat. No.: HY-12885B